Effect of Atropine on Adrenergic Responsiveness of Erythrocyte and Heart Rhythm Variability in Outbred Rats with Stimulation of the Central Neurotransmitter Systems

Abstract

Single injection of muscarinic cholinoceptor blocker atropine (1 mg/kg) to outbred male rats reduced β-adrenergic responsiveness of erythrocytes (by 2.2 times) and the content of epinephrine granules on erythrocytes (by 1.5 times), significantly increased HR and rigidity of the heart rhythm, and manifold decreased the power of all spectral components of heart rhythm variability. Stimulation of the central neurotransmitter systems increased β-adrenergic responsiveness of erythrocytes (by 15-26%), decreased the number of epinephrine granules on erythrocytes (by 25-40%), and increased HR and cardiac rhythm intensity. These changes were most pronounced after stimulation of the serotoninergic system. Administration of atropine against the background of activation of central neurotransmitter systems did not decrease β-adrenergic responsiveness of erythrocytes (this parameter remained at a stably high level and even increased during stimulation of the dopaminergic system), but decreased the number of epinephrine granules on erythrocytes, increased HR, and dramatically decreased the power of all components of heart rhythm variability spectrum. The response to atropine was maximum against the background of noradrenergic system activation and less pronounced during stimulation of the serotoninergic system. Thus, substances that are complementary to cholinergic receptors modulated adrenergic effect on the properties of red blood cells, which, in turn, can modulate the adrenergic influences on the heart rhythm via the humoral channel of regulation. Stimulation of central neurotransmitter systems that potentiates the growth of visceral adrenergic responsiveness weakens the cholinergic modulation of the adrenergic influences, especially with respect to erythrocyte responsiveness. Hence, changes in the neurotransmitter metabolism in the body can lead to coupled modulation of reception and reactivity to adrenergic- and choline-like regulatory factors at the level of erythrocyte membranes, which can be important for regulation of heart rhythm.

Features of Heart Rate Variability in Male and Female Wistar Rats

“…Курьяновой с соавт. при анализе ЧСС, Мо, ИН, HF (%), LF (%) и VLF (%) было указано, что регуляция кардиоритма самок нелинейных крыс осуществляется при более высоких, чем у самцов, адренергических влияниях через гуморальный и нервный каналы регуляции [20]. В связи с высокой долей в спектре ВРС самок волн VLF наряду с волнами HF-диапазона волновая структура сердечного ритма представляется более сложной, чем у самцов [6,15].…”

Heart rate variability in female Wistar rats under conditions of different physical activity

“…В ряде случаев записи кардиосигнала проводятся на иммобилизированных и наркотизированных крысах, что однозначно не позволяет получить данные о состоянии организма и функционировании регуляторных механизмов [4]. В некоторых работах записи проводились на свободно движущихся животных при помощи электродов-зажимов, фиксированных после обезболивания, что также не позволяет рассматривать полученные результаты в качестве критериев физиологической нормы у крыс [5]. Кроме того, на исходный уровень показателей ВСР значительное влияние оказывают фенотипические особенности линии или породы лабораторных животных [6].…”