Effect of atrial natriuretic peptide on α-methyl-d-glucoside intestinal active uptake in rats

Bosc, Laura V. González; Vidal, N. A.; Prieto, Rafel M.; Tur, Josep A.

doi:10.1016/s0196-9781(98)00072-2

Cited by 15 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are various glucose transporters in the intestinal mucosa, and previous studies using membrane vesicles24,25 and isolated cells26 confirmed the predicted polarized distribution of SGLT 1, Na + ‐dependent and GLUT, Na + ‐independent transporters. SGLT 1 was present in the rat colonic mucosa, although its level of expression was lower than those in the jejunum and ileum 27. Moreover, when a glucose analogue was taken up into the mucosal membrane, the Isc value was increased due to the influx of Na + by interaction to SGLT 1 18.…”

Section: Resultsmentioning

confidence: 93%

Regional Intestinal Absorption of FITC–Dextran 4,400 with Nanoparticles Based on β-Sitosterol β-D-Glucoside in Rats

Nakamura

Takayama

Nagai

et al. 2003

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 93%

Regional Intestinal Absorption of FITC–Dextran 4,400 with Nanoparticles Based on β-Sitosterol β-D-Glucoside in Rats

Nakamura

Takayama

Nagai

et al. 2003

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

“… 41 , 42 SGLT1 can actively uptake glucose in rat, pig, and human colons, albeit at a lower rate compared to the small intestine. 43 , 44 This result is intriguing since C. difficile represses toxin production in the presence of glucose in vitro through a mechanism of carbon catabolite repression. 45 , 46 Moreover, a high-carbohydrate diet rich in corn starch, casein, maltodextrin, and sucrose was protective against severe CDI.…”

Section: Discussionmentioning

confidence: 99%

Increased intestinal permeability and downregulation of absorptive ion transporters Nhe3 , Dra , and Sglt1 contribute to diarrhea during Clostridioides difficile infection

et al. 2023

View full text Add to dashboard Cite

Background & Aim Clostridioides difficile infection (CDI) is the leading cause of hospital-acquired diarrhea and pseudomembranous colitis. Two protein toxins, TcdA and TcdB, produced by C. difficile are the major determinants of disease. However, the pathophysiological causes of diarrhea during CDI are not well understood. Here, we investigated the effects of C. difficile toxins on paracellular permeability and apical ion transporters in the context of an acute physiological infection. Methods We studied intestinal permeability and apical membrane transporters in female C57BL/6J mice. Üssing chambers were used to measure paracellular permeability and ion transporter function across the intestinal tract. Infected intestinal tissues were analyzed by immunofluorescence microscopy and RNA-sequencing to uncover mechanisms of transporter dysregulation. Results Intestinal permeability was increased through the size-selective leak pathway in vivo during acute CDI in a 2-day-post infection model. Chloride secretory activity was reduced in the cecum and distal colon during infection by decreased CaCC and CFTR function, respectively. SGLT1 activity was significantly reduced in the cecum and colon, accompanied by ablated SGLT1 expression in colonocytes and increased luminal glucose concentrations. SGLT1 and DRA expression was ablated by either TcdA or TcdB during acute infection, but NHE3 was decreased in a TcdB-dependent manner. The localization of key proteins that link filamentous actin to the ion transporters in the apical plasma membrane was unchanged. However, Sglt1, Nhe3 , and Dra were drastically reduced at the transcript level, implicating downregulation of ion transporters in the mechanism of diarrhea during CDI. Conclusions CDI increases intestinal permeability and decreases apical abundance of NHE3, SGLT1, and DRA. This combination likely leads to dysfunctional water and solute absorption in the large bowel, causing osmotic diarrhea. These findings provide insights into the pathophysiological mechanisms underlying diarrhea and may open novel avenues for attenuating CDI-associated diarrhea.

show abstract

“…On the other hand, α-MG was not hydrolyzed by the intestinal crude enzyme preparation (data not shown). At present, α-MG is considered as a nonmetabolic glucoside because it was not hydrolyzed by mammalian enzymes ( − ). Therefore, α-EG hydrolyzing enzymes in rat small intestine might recognize differences in carbon length between ethyl and methyl in aglycon.…”

Section: Discussionmentioning

confidence: 99%

“…Vitamin glucosides ( , ) and flavonoid glucosides () were completely or incompletely hydrolyzed in the animal body, and then, they provide glucose and aglycons. On the other hand, methyl α- d -glucoside (α-MG), nonmetabolic glucoside, is usable for analyzing the transport activity of sodium-dependent glucose transporters (SGLTs) ( − ).…”

Section: Introductionmentioning

confidence: 99%

Studies on Absorption and Hydrolysis of Ethyl α-d-Glucoside in Rat Intestine

Mishima

Tanaka

Tsuge

et al. 2005

J. Agric. Food Chem.

View full text Add to dashboard Cite

Ethyl alpha-D-glucoside (alpha-EG) is normally contained in Sake, which has been taken by Japanese people since ancient times. In this study, the intestinal absorption of alpha-EG was investigated using rat everted intestinal sac. Furthermore, the alpha-EG hydrolytic activity in rat intestine was compared with disaccharides hydrolytic activities, and the effects of alpha-EG on disaccharides hydrolysis were examined using crude enzyme preparation from rat intestinal acetone powder. Glucose liberated from alpha-EG was detected in a serosal solution of everted rat intestinal sac, but it was only less than 4% of absorbed intact alpha-EG. alpha-EG absorption into small intestinal tissue was reduced by elimination of sodium ion from the mucosal solution or under the presence of phlorizin. The hydrolytic activity for alpha-EG was detected in crude enzyme preparation from rat intestinal acetone powder, but it showed a low value as compared to those for disaccharides. alpha-EG showed mixed type inhibition for maltose and sucrose hydrolysis, but inhibitory concentrations of alpha-EG required for 50% inhibition for the maltose and sucrose hydrolysis were higher than those of arabinose and acarbose. In conclusion, a small amount of alpha-EG was hydrolyzed and most of it was absorbed via SGLT1 as an intact form in the rat small intestine, and the inhibitory effect of alpha-EG on disaccharides hydrolysis was weak.

show abstract

Effect of atrial natriuretic peptide on α-methyl-d-glucoside intestinal active uptake in rats

Cited by 15 publications

References 24 publications

Regional Intestinal Absorption of FITC–Dextran 4,400 with Nanoparticles Based on β-Sitosterol β-D-Glucoside in Rats

Regional Intestinal Absorption of FITC–Dextran 4,400 with Nanoparticles Based on β-Sitosterol β-D-Glucoside in Rats

Increased intestinal permeability and downregulation of absorptive ion transporters Nhe3 , Dra , and Sglt1 contribute to diarrhea during Clostridioides difficile infection

Studies on Absorption and Hydrolysis of Ethyl α-d-Glucoside in Rat Intestine

Contact Info

Product

Resources

About