Effect of atrazine and zearalenone on the numer of receptor binding sites for 3H-estradiol in the rat uterus cytosol

Abstract

The effect of a daily dose of 14 mg atrazine and/or 2.5 mg zearalenone that were administered to rats during 5 days of estrous cycle on the number of receptor binding sites for 3H-estradiol in uterine cytosol was investigated. Comparison with a control group of animals showed no effect of atrazine influence on the number of receptor binding sites for 3H-estradiol in the rat uterine cytosol until day 6 of the experiment, when a significantly lower value was recorded (P < 0.05). In the group of animals administered zearalenone, a decline in the number of receptor binding sites for 3H-estradiol in uterine cytosol was observed. On day 4 of the experiment, the number of binding sites was significantly greater (P < 0.05) in this group than in the control group of animals, whereafter it gradually decreased to a significantly lower value (P < 0.05) as compared to the control group on day 6. In the group of animals administered a mixture of atrazine and zearalenone, the number of receptor binding sites for 3H-estradiol in uterine cytosol did not decrease but showed a similar pattern as in the control group of animals, however, at a statistically significantly (P < 0.05) higher level.

Zearalenone (ZEN) and Its Influence on Regulation of Gene Expression in Carp (Cyprinus carpio L.) Liver Tissue

“…However, the expression of ERα and ERβ2, which is usually high in carp livers [ 50 ], was found to be reduced after exposing the fish to ZEN. It is known that ZEN competes with natural estrogen for the ER binding sites in the uterus of rats [ 51 ]. However, and in contrast to higher vertebrates, detailed data on the effects of phyto- and mycoestrogens on the expression of the various ER subtypes in fish is lacking and knowledge of endogenous estrogens is very limited.…”

Impact of Fusarium-Derived Mycoestrogens on Female Reproduction: A Systematic Review

“…Receptor-binding studies. Molecular studies in pigs, rats, and mice examining mycoestrogen exposure and receptor binding showed binding to ERα and ERβ in the uterus [ 15 , 16 , 17 , 18 ]. Some evidence in rats suggests impacts of ZEN on estrogen receptor density in the uterus as well [ 16 ].…”

“…The estrogenic potency of mycoestrogens has also been demonstrated in vivo. For example, in mice, rats, and pigs, ZEN and metabolites readily bind uterine ERα and ERβ receptors [ 15 , 16 , 17 , 18 ]. Pig studies further suggest that zeranol has a higher affinity to ERα than ZEN or the metabolites α- and β-ZOL [ 17 ].…”