Effect of assembly pH and ionic strength of chitosan/casein multilayers on benzydamine hydrochloride release

Marudova, Maria; Exner, Ginka; Pilicheva, Bissera; Marinova, A.; Viraneva, A.; Bodurov, I; Sotirov, Sotir; Vlaeva, I.; Uzunova, Yordanka; Yovcheva, T.

doi:10.1080/00914037.2018.1525727

Cited by 6 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The thickness of the coating and the total drug loading in the coating depend largely on the ionic strength of the polyelectrolyte solutions, their concentration, and the pH. We have selected the well-studied polycations CS and PLL, which are most often used in combination with γ-PGA. ,, The ionic strength of the polyelectrolyte solutions was set at 100 mM, which is optimal to provide good adsorption of the polyelectrolytes, their electrostatic interactions, and sufficient coating thickness. , The pH of the CS solution was adjusted to 5.0, which ensures good ionization of the chitosan chain since its p K a is 6.3 (calculated by potentiometric titration, Figure S4A). For the deposition of chitosan-based coating, the pH of γ-PGA and conjugate DOX/γ-PGA solutions were adjusted to 6.3, providing a sufficiently ionized molecule and a comfortable pH for the subsequent chitosan layer (Figure S4B).…”

Section: Resultsmentioning

confidence: 99%

“…This method consists of multiple layer-by-layer adsorptions of polyelectrolytes and the formation of an electrostatic complex between the polyelectrolytes due to ionic and/or hydrogen bonding. Varying the adsorption parameters (pH, ionic strength, and polyelectrolyte concentration of the solutions) results in different platform architectures and the ability to control structural properties such as thickness, permeability, and pore size. , In turn, the incorporation of drugs into such platforms allows the kinetics and duration of drug release to be controlled, as drug release is largely due to the hydrolysis of polyelectrolytes and the electrostatic complex between them. , The field of controlled delivery of antineoplastic agents still has significant potential for improvement. The most crucial areas for improvement identified are reducing explosive release and increasing the level of prolonged release, which were the primary objectives of our study.…”

Section: Introductionmentioning

confidence: 99%

“…Varying the adsorption parameters (pH, ionic strength, and polyelectrolyte concentration of the solutions) results in different platform architectures and the ability to control structural properties such as thickness, permeability, and pore size. 15,16 In turn, the incorporation of drugs into such platforms allows the kinetics and duration of drug release to be controlled, as drug release is largely due to the hydrolysis of polyelectrolytes and the electrostatic complex between them. 12,17 The field of controlled delivery of antineoplastic agents still has significant potential for improvement.…”

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradable Local Chemotherapy Platform with Prolonged and Controlled Release of Doxorubicin for the Prevention of Local Tumor Recurrence

Voznyuk,

Makarets,

Advakhova

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Local recurrence after surgical and therapeutic treatment remains a significant clinical problem in oncology. Recurrence may be due to imperfections in existing therapies, particularly chemotherapy. To improve antitumor activity and prevent local cancer recurrence while keeping toxicity at acceptable levels, we have developed and demonstrated a biodegradable local chemotherapy platform that provides controlled and prolonged drug release. The platform consists of a polycaprolactone (PCL) substrate, which provides the structural integrity of the platform and the predominant unidirectional drug release, and a thin multilayer coating (∼200 nm) containing doxorubicin (DOX). The coating is an electrostatic complex obtained by the layer-bylayer (LbL) assembly and consists of natural polyelectrolytes [poly-γ-glutamic acid (γ-PGA) and chitosan (CS) or poly-L-lysine (PLL)]. To improve the release stability, an ionic conjugate of DOX and γ-PGA was prepared and incorporated into the multilayer coating. By varying the structure of the coating by adding empty (without DOX) bilayers, we were able to control the kinetics of drug release. The resulting platforms contained equal numbers of empty bilayers and DOX-loaded bilayers (15 + 15 or 30 + 30 bilayers) with a maximum loading of 566 ng/cm 2 . The platforms demonstrated prolonged and fairly uniform drug release for more than 5 months while retaining antitumor activity in vitro on ovarian cancer cells (SKOV-3). The empty platforms (without DOX) showed good cytocompatibility and no cytotoxicity to human fibroblasts and SKOV-3 cells. This study presents the development of a local chemotherapy platform consisting of a PCL-based substrate which provides structural stability and a biodegradable polyelectrolyte layered coating which combines layers containing a polyanion ionic complex with DOX with empty bilayers to ensure prolonged and controlled drug release. Our results may provide a basis for improving the efficacy of chemotherapy using drug delivery systems.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biodegradable Local Chemotherapy Platform with Prolonged and Controlled Release of Doxorubicin for the Prevention of Local Tumor Recurrence

Voznyuk,

Makarets,

Advakhova

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…As the pH is increased above the IEP, the chain–chain spacing diminishes, leading to the restoration of the matrix stiffness and reduction in the average pore size. Similar structural alterations can occur depending on the ionic strength of the medium in which chitosan molecules are dispersed, in such a way that higher charge densities in the solution promote the charge screening effect and impel chitosan to adopt a stiffer form with a hampered release behavior [ 25 ]. Another major positive feature of chitosan molecules is that they contain a high density of reactive hydroxyl and amine groups that can chemically attach to ligands [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Tunable Release of Calcium from Chitosan-Coated Bioglass

Uskoković,

Abuna,

Hampton

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

Bioglass presents a standard biomaterial for regeneration of hard tissues in orthopedics and dentistry. The notable osteo-inductive properties of bioglass are largely due to the release of calcium ions from it. However, this release is not easily controllable and can often be excessive, especially during the initial interaction of the biomaterial with the surrounding tissues. Consequently, this excessive release can deplete the calcium content of the bioglass, ultimately reducing its overall bioactivity. In this study, we have tested if applying biopolymer chitosan coatings of different thicknesses would be able to mitigate and regulate the calcium ion release from monodisperse bioglass nanoparticles. Calcium release was assessed for four different chitosan coating thicknesses at different time points over the period of 28 days using a fluorescence quencher. Expectedly, chitosan-coated particles released less calcium as the concentration of chitosan in the coating solution increased, presumably due to the increased thickness of the chitosan coating around the bioglass particles. The mechanism of release remained constant for each coating thickness, corresponding to anomalous, non-Fickian diffusion, but the degree of anomalousness increased with the deposition of chitosan. Zeta potential testing showed an expected increase in the positive double layer charge following the deposition of the chitosan coating due to the surface exposure of the amine groups of chitosan. Less intuitively, the zeta potential became less positive as thickness of the chitosan coating increased, attesting to the lower density of the surface charges within thicker coatings than within the thinner ones. Overall, the findings of this study demonstrate that chitosan coating efficiently prevents the early release of calcium from bioglass. This coating procedure also allows for the tuning of the calcium release kinetics by controlling the chitosan concentration in the parent solution.

show abstract

“…When deployed for wound treatment, the architecture of the PEM film can be designed to give a burst release [15][16][17] or slow diffusion of the peptides from the film [18]. Delivery of target molecules can be partially controlled through changing the conditions of multilayer assembly including ionic strength [19], pH [16,20,21], temperature [22] and polyelectrolyte species [8]. In addition, PEMs can be applied to many surfaces including flat planes [23], nanoparticles [24] and nanocapsules [25,26], and three-dimensional (3D) porous scaffolds [27].…”

Section: Introductionmentioning

confidence: 99%

Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers

Benbow

Karpiniec²,

Krasowska

et al. 2020

Marine Drugs

View full text Add to dashboard Cite

Biopolymer polyelectrolyte multilayers are a commonly studied soft matter system for wound healing applications due to the biocompatibility and beneficial properties of naturally occurring polyelectrolytes. In this work, a popular biopolymer, chitosan, was combined with the lesser known polysaccharide, fucoidan, to create a multilayer film capable of sequestering growth factor for later release. Fucoidan has been shown to act as a heparin-mimic due to similarities in the structure of the two molecules, however, the binding of fibroblast growth factor-2 to fucoidan has not been demonstrated in a multilayer system. This study assesses the ability of fucoidan to bind fibroblast growth factor-2 within a fucoidan/chitosan polyelectrolyte multilayer structure using attenuated total internal reflectance infrared spectroscopy and quartz crystal microbalance with dissipation monitoring. The fibroblast growth factor-2 was sequestered into the polyelectrolyte multilayer as a cationic layer in the uppermost layers of the film structure. In addition, the diffusion of fibroblast growth factor-2 into the multilayer has been assessed.

show abstract

Effect of assembly pH and ionic strength of chitosan/casein multilayers on benzydamine hydrochloride release

Cited by 6 publications

References 34 publications

Biodegradable Local Chemotherapy Platform with Prolonged and Controlled Release of Doxorubicin for the Prevention of Local Tumor Recurrence

Biodegradable Local Chemotherapy Platform with Prolonged and Controlled Release of Doxorubicin for the Prevention of Local Tumor Recurrence

Tunable Release of Calcium from Chitosan-Coated Bioglass

Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers

Contact Info

Product

Resources

About