Effect of aspiration vacuum on the developmental competence of immature human oocytes retrieved using a 20-gauge needle

Hashimoto, Shu; Fukuda, Aisaku; Murata, Yasutaka; Kikkawa, M.; Oku, Hirotsugu; Kanaya, Hiroyuki; Sonoda, M.; Sugihara, K.; Murata, Toshiaki; Nagata, Fumie; Nakaoaka, Yoshiharu; Morimoto, Yoshiharu

doi:10.1016/s1472-6483(10)60891-7

Cited by 28 publications

(18 citation statements)

References 29 publications

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“…The percentage of COCs of retrieved oocytes in 180 mmHg (70%) was significantly higher (P<0.05) than that of oocytes in 300 mmHg (46%, Fig. 5) [54]. Moreover, the developmental rate of oocytes obtained by lower aspiration vacuum to transferable embryos (24%, Fig.…”

Section: Application To Human Artmentioning

confidence: 84%

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Application of In Vitro Maturation to Assisted Reproductive Technology

Hashimoto¹

2009

J. Reprod. Dev.

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Abstract. In vitro maturation (IVM) of immature oocytes is an important reproductive technology that generates mature oocytes that are capable of supporting preimplantation embryo development and full development to turm. The developmental competence of oocytes after in vitro fertilization (IVF) was supported in cumulus-cell density dependent manner. The developmental competence of oocytes matured under low oxygen (5% O2) tension was higher than that of oocytes matured under high oxygen tensinon (20% O2) due to the decrease of reactive oxygen content in the oocyte. To expand the availability of female gamete, an attempt to culture the growing oocytes have been made. We have focused attention on the mitochondrial activity of in vitro grown (IVG) oocytes and shown that L-Carnitine, which acts as a carrier of fatty acids across the inner mitochondrial membrane for subsequent β-oxidation, supported the viability of growing oocytes. Nowadays, IVM is gaining acceptance in assisted reproductive technology in cattle and human. Key words: Cumulus cell density, Meiotic arrest, Mitochondrial activity of growing oocytes, Oocyte transportation, Transvaginal oocyte pick up (J. Reprod. Dev. 55: [1][2][3][4][5][6][7][8][9][10] 2009) nimal breeders, especially cattle breeders have been trying to get as many offspring from their genetically superior or important animals as possible. Thus, hormonal induction of multiple ovulations, followed by non-surgical collection of the embryos and embryo transfer to recipients is widely used. This multiple ovulation and embryo transfer procedure can be repeated in the same animal about 4 times per year in cattle. However, the yield of transferable embryos is highly variable. No more than an average of 20 embryos per year per animal can be obtained in cattle [1]. Feedback on the endocrinological regulation of follicle growth and maturation also limits the number of oocytes that can mature and develop in vivo [2]. Thus embryo production in vivo by means of super-ovulation may lead to less than 100 embryos over the lifespan of an individual cow, whereas approximately 100,000 gametes are present in the ovaries. If more gametes could be matured, fertilized and developed in vitro, production would increase correspondingly. Oocytes from slaughterhouse ovaries [3][4][5] have been used for the production of bovine embryos in vitro. Moreover, in vitro embryo production from live cows has been established [6]. However, the ability of bovine oocytes matured in vitro to develop to the morula/blastocyst stage is less than that of in vivo-matured oocytes [7,8]. The decrease in the developmental competence of in vitro-matured oocytes may be due to insufficient cytoplasmic maturity.In this paper, 1) bovine oocyte-pick-up (OPU) from live cows, 2) transportation of bovine immature oocytes, 3) improvement of in vitro maturation (IVM) of bovine immature oocytes, 4) application of IVM culture to human assisted reproductive technology (ART), 5) IVM following meiotic inhibition with butyrolactone I under low oxyg...

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Section: Application To Human Artmentioning

confidence: 84%

“…Fig. 5) [54]. Moreover, the developmental rate of oocytes obtained by lower aspiration vacuum to transferable embryos (24%, Fig.…”

mentioning

confidence: 93%

Application of In Vitro Maturation to Assisted Reproductive Technology

Hashimoto¹

2009

J. Reprod. Dev.

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“…Good quality embryos (GQE) were evaluated on day 3 taking into account the number, symmetry and granularity of blastomeres; type and the percentage of fragmentation; and presence of multinucleated blastomeres as reported previously [18]. Blastocysts were scored on day 5 and 6 according to expansion of the blastocoele cavity, degree of hatching and number and integrity of both the inner cell mass (ICM) and trophoectoderm cells, as described previously [19].…”

Section: Embryo Morphology and Quality Assessmentmentioning

confidence: 99%

“…Embryo transfer (ET), cryopreservation, endometrial preparation in hormone replacement cycles (HRC) and pregnancy diagnosis were performed as previously reported [18]. Briefly, ET was carried out using an ET catheter (Kitazato Supply, Shizuoka, Japan).…”

Section: Embryo Transfer Cryopreservation Endometrial Preparation Amentioning

confidence: 99%

Medium without Ammonium Accumulation Supports the Developmental Competence of Human Embryos

Hashimoto¹,

Nishihara²,

Murata³

et al. 2008

J. Reprod. Dev.

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Abstract. L-Glutamine has been shown to play an important role during in vitro culture of mammalian embryos. However, it is easily decomposed into ammonium, which is believed to have deleterious effects on preimplantation embryos. In this study, we assessed prospectively the developmental competence of human embryos cultured in medium containing L-glutamine or a novel stable glutamine derivative and vitamins. The subjects of this study were 41 women who underwent IVF/ET treatment from September to November 2006 and from whom 6 or more oocytes were retrieved. Sibling oocytes were randomly divided into EA/BA (EmbryoAssyst ® and BlastAssyst ® containing a novel stable glutamine derivative and vitamins), and BAS groups (BlastAssyst ® system containing L-glutamine). There was no difference in pronuclear formation rate between EA/BA and BAS (74 vs. 69%). The blastulation rates of embryos based on the number of zygotes cultured in EA/BA on Days 5 (Day 0=insemination, 54%) and 6 (63%) were significantly higher (P<0.05) than those cultured in BAS (Day 5: 33% and Day 6: 45%, respectively). The present data indicate that a medium containing a novel stable glutamine derivative and vitamins supports the developmental competence of human embryos.

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“…In one of the very few studies available in humans, Hashimoto et al (2007) evaluated the effects of various aspiration vacuums during oocyte retrieval with a 20-gauge single lumen needle on the developmental capability of immature oocytes after in vitro maturation (IVM), IVF and embryo transfer (Hashimoto et al, 2007). Immature oocytes were aspirated by 20-gauge single lumen needle with a pressure of 180 or 300 mmHg.…”

Section: Aspiration Of Oocytesmentioning

confidence: 99%

Charting new frontiers in In Vitro Fertilization (IVF): The Role of Bioengineering

Carneiro

Lamaita

Barbosa

et al. 2015

JBRA Assisted Reproduction

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Since the beginning of in vitro fertilization (IVF) 36 years ago, scientists have studied and critically analyzed the techniques in order to find ways to improve outcomes. However, success rates vary significantly among clinics due to poor reproducibility and inconsistency across operators. Much research has been conducted on the chemical environment, or culture medium, surrounding the oocyte/ embryo, but little attention has been given to the actual equipment and physical culture environment, which has changed very little over the years. The aim of this paper was to evaluate how the physical factors are important regulators of oocyte and embryo function and to improve understanding of the physical forces involved in the processes in human reproduction. A review the available literature was conducted using PubMed from 1966 through July 2014 in an attempt to help integrate mechanics into our understanding of the molecular basis of IVF. Keywords included in vitro fertilization, biomechanics, bioengineering, oocyte and embryo. The mechanical characterization of oocytes and embryos represents an opportunity to detect cellular defects, assess quality and bio-viability of processes such as cryopreservation as well as select the best embryo for transfer. Defining the mechanical forces at play during embryo transfer is also an important step towards improving results in in vitro fertilization. The further analysis of these phenomena needs a detailed monitoring of the mechanical conditions and more extensive studies of events on the cellular and molecular levels.

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Effect of aspiration vacuum on the developmental competence of immature human oocytes retrieved using a 20-gauge needle

Cited by 28 publications

References 29 publications

Application of In Vitro Maturation to Assisted Reproductive Technology

Application of In Vitro Maturation to Assisted Reproductive Technology

Medium without Ammonium Accumulation Supports the Developmental Competence of Human Embryos

Charting new frontiers in In Vitro Fertilization (IVF): The Role of Bioengineering

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