Effect of Ashwagandha Withanolides on Muscle Cell Differentiation

Wang, Jia; Zhang, Huayue; Kaul, A.; Li, Kejuan; Priyandoko, Didik; Kaul, Sunil C.; Wadhwa, Renu

doi:10.3390/biom11101454

Cited by 6 publications

(3 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Withanone, a specific withanolide present in ashwagandha extract, induced a more robust differentiation of myoblasts into myotubes. It also facilitated the deaggregation of proteins that had formed aggregates due to heat and metal stress, concurrently activating hypoxia and autophagy pathways [ 57 ]. The differentiation of myoblasts necessitates functional degradative systems, including autophagy, which play a role in the formation of multinucleated terminally differentiated myotubes.…”

Section: Discussionmentioning

confidence: 99%

Ashwagandha Ethanol Extract Attenuates Sarcopenia-Related Muscle Atrophy in Aged Mice

Ko,

Chang,

Choi

et al. 2024

Nutrients

View full text Add to dashboard Cite

The investigation focused on the impact of Withania somnifera (ashwagandha) extract (WSE) on age-related mechanisms affecting skeletal muscle sarcopenia-related muscle atrophy in aged mice. Beyond evaluating muscular aspects, the study explored chronic low-grade inflammation, muscle regeneration, and mitochondrial biogenesis. WSE administration, in comparison to the control group, demonstrated no significant differences in body weight, diet, or water intake, affirming its safety profile. Notably, WSE exhibited a propensity to reduce epidermal and abdominal fat while significantly increasing muscle mass at a dosage of 200 mg/kg. The muscle-to-fat ratio, adjusted for body weight, increased across all treatment groups. WSE administration led to a reduction in the pro-inflammatory cytokines TNF-α and IL-1β, mitigating inflammation-associated muscle atrophy. In a 12-month-old mouse model equivalent to a 50-year-old human, WSE effectively preserved muscle strength, stabilized grip strength, and increased muscle tissue weight. Positive effects were observed in running performance and endurance. Mechanistically, WSE balanced muscle protein synthesis/degradation, promoted fiber differentiation, and enhanced mitochondrial biogenesis through the IGF-1/Akt/mTOR pathway. This study provides compelling evidence for the anti-sarcopenic effects of WSE, positioning it as a promising candidate for preventing sarcopenia pending further clinical validation.

show abstract

Section: Discussionmentioning

confidence: 99%

Ashwagandha Ethanol Extract Attenuates Sarcopenia-Related Muscle Atrophy in Aged Mice

Ko,

Chang,

Choi

et al. 2024

Nutrients

View full text Add to dashboard Cite

show abstract

“…In addition, various parts of the plant, including the roots, leaves, stems, and flowers, have therapeutic value, and more than twenty-nine types of bioactive compounds have been extracted from the roots and leaves alone [ 12 , 13 , 14 ]. Withaferin A, in both in vitro and in vivo studies, has shown unique therapeutic properties, including antioxidant, anti-inflammatory, antibacterial, antistress, antidiabetic, antipyretic, cardioprotective, neuroprotective, and anticancer activities [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. The production of withaferin A from natural sources has stimulated research in various areas of chemical sciences, chemical biology, biotechnology, biochemistry, microbiology, and organic chemistry.…”

Section: Introductionmentioning

confidence: 99%

Withaferin A: A Pleiotropic Anticancer Agent from the Indian Medicinal Plant Withania somnifera (L.) Dunal

Kumar

Mathew

Aharwal³

et al. 2023

Pharmaceuticals

View full text Add to dashboard Cite

Cancer represents the second most deadly disease and one of the most important public health concerns worldwide. Surgery, chemotherapy, radiation therapy, and immune therapy are the major types of treatment strategies that have been implemented in cancer treatment. Unfortunately, these treatment options suffer from major limitations, such as drug-resistance and adverse effects, which may eventually result in disease recurrence. Many phytochemicals have been investigated for their antitumor efficacy in preclinical models and clinical studies to discover newer therapeutic agents with fewer adverse effects. Withaferin A, a natural bioactive molecule isolated from the Indian medicinal plant Withania somnifera (L.) Dunal, has been reported to impart anticancer activities against various cancer cell lines and preclinical cancer models by modulating the expression and activity of different oncogenic proteins. In this article, we have comprehensively discussed the biosynthesis of withaferin A as well as its antineoplastic activities and mode-of-action in in vitro and in vivo settings. We have also reviewed the effect of withaferin A on the expression of miRNAs, its combinational effect with other cytotoxic agents, withaferin A-based formulations, safety and toxicity profiles, and its clinical potential.

show abstract

“…WA has been reported to possess a variety of therapeutic properties, including antioxidant, anti-inflammatory, antibacterial, antistress, antidiabetic, antipyretic, cardioprotective, neuroprotective, and anticancer activities [ 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. The possibility of using WA as a broadly active cancer therapeutic has attracted particular attention and over the years has resulted in a multitude of studies employing various in vitro and in vivo cancer models (reviewed in, e.g., in refs.…”

Section: Introductionmentioning

confidence: 99%

Withaferin A and Celastrol Overwhelm Proteostasis

Vilaboa,

Voellmy

2023

IJMS

View full text Add to dashboard Cite

Withaferin A (WA) and celastrol (CEL) are major bioactive components of plants that have been widely employed in traditional medicine. The pleiotropic activities of plant preparations and the isolated compounds in vitro and in vivo have been documented in hundreds of studies. Both WA and CEL were shown to have anticancer activity. Although WA and CEL belong to different chemical classes, our synthesis of the available information suggests that the compounds share basic mechanisms of action. Both WA and CEL bind covalently to numerous proteins, causing the partial unfolding of some of these proteins and of many bystander proteins. The resulting proteotoxic stress, when excessive, leads to cell death. Both WA and CEL trigger the activation of the unfolded protein response (UPR) which, if the proteotoxic stress persists, results in apoptosis mediated by the PERK/eIF-2/ATF4/CHOP pathway or another UPR-dependent pathway. Other mechanisms of cell death may play contributory or even dominant roles depending on cell type. As shown in a proteomic study with WA, the compounds appear to function largely as electrophilic reactants, indiscriminately modifying reachable nucleophilic amino acid side chains of proteins. However, a remarkable degree of target specificity is imparted by the cellular context.

show abstract

Effect of Ashwagandha Withanolides on Muscle Cell Differentiation

Cited by 6 publications

References 87 publications

Ashwagandha Ethanol Extract Attenuates Sarcopenia-Related Muscle Atrophy in Aged Mice

Ashwagandha Ethanol Extract Attenuates Sarcopenia-Related Muscle Atrophy in Aged Mice

Withaferin A: A Pleiotropic Anticancer Agent from the Indian Medicinal Plant Withania somnifera (L.) Dunal

Withaferin A and Celastrol Overwhelm Proteostasis

Contact Info

Product

Resources

About