Effect of ascorbic acid-rich diet onin vivo-induced oxidative stress

Abstract

Using hyperbaric oxygen (HBO) therapy as an in vivo oxidation model, we investigated the effect of a diet enriched in ascorbic acid (AA) on HBO-induced oxidative stress. Volunteers (n 46) were allocated to the AA-rich diet group or the control group. Blood samples were collected at the basal time, after the 1-week diet before and immediately after the HBO treatment, and 1 week after the HBO treatment. AA level, total antioxidant status (TAS), hydroperoxides (HP), lymphocyte DNA oxidation and DNA repair capacity were assessed. The expression of genes involved in oxidative stress was evaluated in lymphocytes and the protein activity of the modulated genes was determined in the plasma. The AA level and the antioxidant status of plasma were increased by AA-rich food consumption. HBO exposure did not affect the AA levels or TAS, but induced HP formation in the control group. The lymphocytes isolated from dietary-supplemented subjects were resistant to ex vivo DNA oxidation, showing an increased DNA repair capacity compared with controls. A difference in gene expression pattern was observed between the groups. AA-rich foods provide dual protection against oxidative stress, enhancing plasma antioxidant levels and stimulating genes involved in cell detoxification.

Dietary Phytochemicals Promote Health by Enhancing Antioxidant Defence in a Pig Model

“…In comparison to plasma TEAC, a delayed increase in plasma GPx activity was observed at 1.5 h after consumption of red cabbage (2.22 mg GAE/kg BW) and at 2.5, 3.5, and 4 h after consumption of grape skin (4.44 mg GAE/kg BW). The observed time delay of plasma GPx activity after plasma TEAC is consistent with a previous study [ 68 ]. This delay may be explained by the induction of GPx activity occurring in response to the presence of phytochemicals in the plasma, as indicated by increased plasma TEAC [ 58 , 59 ].…”

Organic honey supplementation reverses pesticide‐induced genotoxicity by modulating DNA damage response

“…In the last decade, a number of studies have shown that nutrients can affect metabolic traits by altering the structure of chromatin and directly regulate both transcription and translational processes . In this context, dietary polyphenol‐targeted epigenetics becomes an attractive approach for disease prevention and intervention . Here, we found that honey‐polyphenols modulate the activity of DNA repair involved in the DNA base excision repair (BER) system.…”

Genistein and Ascorbic Acid Reduce Oxidative Stress-Derived DNA Damage Induced by the Antileishmanial Meglumine Antimoniate

“…These authors demonstrated that ascorbic acid is able to increase expression of SOD3 (encoding superoxide dismutase 3) and OGG1 (encoding 8-oxoguanine DNA glycosylase) by activation of NRF2 (factor 2 nuclear transcription factor related to erythroid 2). Moreover, Alevva et al (56) have verified that dietetic ingestion of ascorbic acid (600 mg/day) in cells from volunteers treated with hyperbaric oxygen (HBO) induced the expression of important genes for cell detoxification, such as GSTZ1 (encoding glutathione S-transferase zeta 1), genes encoding multifunctional enzymes important to detoxification of electrophilic molecules, and NUDT1 (encoding nucleoside diphosphate linked moiety X motif 1), a gene encoding 7,8-di-hydro-8oxoguanine triphosphatase, which hydrolyzes oxidized purines. Additionally, Cooke et al (57) observed a significant decrease of 8-oxo-G in DNA of cells from volunteers supplemented with ascorbic acid (500 mg/day), thus highlighting that ascorbic acid probably stimulates repairs to oxidized bases in DNA due to its redox properties.…”