Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat

Saha, Beauty

doi:10.3329/bjp.v1i2.491

Cited by 9 publications

(6 citation statements)

References 5 publications

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“…All the tubes were incubated at 37 C and reaction was stopped at different intervals (1.0 hour, 1.5 hours and 2.0 hours) by snap cooling (0-4 C). 16 After cooling the tissues were homogenized and used for the estimation of superoxide dismutase and catalase activity, lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxide (NO) production.…”

Section: Preparation Of Isolated Liver Tissues and Experimental Designmentioning

confidence: 99%

Supplementation of ascorbic acid prevents oxidative damages in arsenic-loaded hepatic tissue of rat: An ex vivo study

et al. 2010

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Oxidative stress due to arsenic toxicity and ameliorative potentiality of L-ascorbic acid was evaluated in an ex vivo system of rat hepatic tissue. The study revealed that arsenic increased the activity of superoxide dismutase (SOD) and catalase (CAT) and the level of lipid peroxidation (LPO), protein carbonyl (PC) and nitric oxide (NO) at 1 hour, 1.5 hours and 2 hours of incubation. Co-treatment with L-ascorbic acid was found effective to normalize the activity of SOD and CAT and the production of LPO, PC and NO in hepatic tissue. This ex vivo study suggested that ascorbic acid is helpful to ameliorate arsenic-induced oxidative stress. This may be one of the alternative screening systems to study the efficacy of antioxidant and hepatoprotective agent.

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Section: Preparation Of Isolated Liver Tissues and Experimental Designmentioning

confidence: 99%

Supplementation of ascorbic acid prevents oxidative damages in arsenic-loaded hepatic tissue of rat: An ex vivo study

et al. 2010

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“…Until now, about 38,000 cases of arsenicosis patients have been identified, though unfortunately still there is no specific treatment. The supportive treatments for arsenicosis are retinol, β-carotene 2 , ascorbic acid 3 , α-tocopherol 4 , zinc 5 , selenium 6 and spirulina 7 . All of these agents are antioxidants and require long duration (4-12 months) of treatment.…”

Section: Introductionmentioning

confidence: 99%

Corn extracts lower tissue arsenic level in rat

Nj¹,

Misbahuddin²,

Ms³

2009

Bangladesh Med Res Counc Bull

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This study was carried out to see whether corn extracts could reduce the accumulation of arsenic in different tissues of rat. Exposure to arsenic (700µg/rat/day) orally for 15 days led to significant accumulation of arsenic and significant reduction in the concentration of reduced glutathione (GSH) in different tissues. While water, salt, ethanol and alkali extracts of corn were co-administered at a dose of 0.5 mL/rat/day orally by stomach tube during last 8 days, arsenic concentration decreased significantly in all tissues and reduced glutathione (GSH) concentration increased significantly in tissues except heart and skin. Among the extracts, water extract produced maximum reduction of arsenic (69.07% in liver, 64.98% in kidney, 63.47% in lung, 57.55% in heart and 69.30% in skin) and elevation of reduced glutathione level in all tissues (17.03% in liver, 46.73% in lung, 32.67% in heart and 55.38% in skin) except kidney, in which maximum elevation of reduced glutathione was attained by ethanol extract (23.14%). This study suggests that corn extracts might protect rats from accumulation of arsenic in different tissues and oxidative stress, which is reflected by the increasing reduced glutathione concentration in those tissues.

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“…Significant reduction in hepatic GSH has been reported in animals receiving sodium meta-arsenite (25 ppm) in drinking water (24 weeks) [67]. Reduced GSH in the liver tissue of rats treated with arsenic trioxide was reversed by the administration of ascorbic acid, suggesting the role of antioxidants in ameliorating arsenic induced oxidative stress [68]. …”

Section: Discussionmentioning

confidence: 99%

Preliminary morphological and biochemical changes in rat liver following postnatal exposure to sodium arsenite

2012

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The effects of sodium arsenite exposure on the hepatic maturation period of cellular and functional reorganization in developing rat livers were evaluated. Animals received intraperitoneal injections of sodium arsenite (1.5 mg/kg body weight) or distilled water on days 9 to 28 after birth. On day 29, the animals were sacrificed either by cervical dislocation or by perfusion fixation. The perfusion fixed liver tissue was processed for paraffin embedding, sectioning and hematoxylin and eosin staining. The fresh liver tissue was processed for cryo-sectioning followed by Sudan Black B staining and for biochemical estimation of reduced glutathione. Microscopic observation revealed comparable preserved hepatic lobular patterns and distributions of uninucleate and binucleate hepatocytes in the control and the experimental groups. The mean nuclear area and diameter of the hepatocytes was increased in the experimental group. Lipid droplet distribution pattern in Sudan Black B stained sections revealed higher staining intensity towards the centrilobular area in both groups. Semiquantitative estimation of staining intensity showed lower mean gray values in zone 3 than in zones 2 and 1 (suggestive of the setting in of the adult pattern) in both groups. The reduced glutathione levels in the liver tissue and the altered nuclear size of the hepatocytes in the experimental group suggested the impairment of morphological and biochemical processes induced by arsenic exposure during the postnatal period.

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Effect of ascorbic acid on reduced glutathione level in arsenic-loaded isolated liver tissues of rat

Cited by 9 publications

References 5 publications

Supplementation of ascorbic acid prevents oxidative damages in arsenic-loaded hepatic tissue of rat: An ex vivo study

Supplementation of ascorbic acid prevents oxidative damages in arsenic-loaded hepatic tissue of rat: An ex vivo study

Corn extracts lower tissue arsenic level in rat

Preliminary morphological and biochemical changes in rat liver following postnatal exposure to sodium arsenite

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