Effect of Ascorbic Acid on Differentiation, Secretome and Stemness of Stem Cells from Human Exfoliated Deciduous Tooth (SHEDs)

Bhandi, Shilpa; Alkahtani, Ahmed; Mashyakhy, Mohammed; Abu-Melha, Abdulaziz Saad; Albar, Nassreen Hassan Mohammad; Renugalakshmi, Apathsakayan; Alkahtany, Mazen F.; Robaian, Ali; Almeslet, Asma; Patil, Vikrant R; Varadarajan, Saranya; Balaji, Thodur Madapusi; Reda, Rodolfo; Testarelli, Luca; Patil, Shankargouda

doi:10.3390/jpm11070589

Cited by 29 publications

(31 citation statements)

References 62 publications

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“…In the present study, significant amounts of SDF‐1 and VEGF released by the scaffold and both factors were detected during all experimental times until 28 days. However, VEGF continued with increasing release rates (similar to Luminex assay), while SDF‐1 stabilized after 48 h. Studies that evaluated the composition of SHED‐secretome showed that VEGF is in greater quantity than SDF‐1, which justifies its increasing release pattern for a longer time, and was what we observed in the Luminex assay as well, with VEGF being one of the most abundant factors released (Bhandi et al., 2021).…”

Section: Discussionsupporting

confidence: 79%

Pro‐angiogenic potential of a functionalized hydrogel scaffold as a secretome delivery platform: An innovative strategy for cell homing‐based dental pulp tissue engineering

Carvalho

Sarra

Schröter

et al. 2022

J Tissue Eng Regen Med

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Angiogenesis is a key process that provides a suitable environment for successful tissue engineering and is even more crucial in regenerative endodontic procedures, since the root canal anatomy limits the development of a vascular network supply.Thus, sustainable and accelerated vascularization of tissue-engineered dental pulp constructs remains a major challenge in cell homing approaches. This study aimed to functionalize a chitosan hydrogel scaffold (CS) as a platform loaded with secretomes of stem cells from human exfoliated deciduous teeth (SHEDs) and evaluate its bioactive function and pro-angiogenic properties. Initially, the CS was loaded with SHED secretomes (CS-S), and the release kinetics of several trophic factors were assessed. Proliferation and chemotaxis assays were performed to analyze the effect of functionalized scaffold on stem cells from apical papilla (SCAPs) and the angiogenic potential was analyzed through the Matrigel tube formation assay with cocultured of human umbilical vein endothelial cells and SCAPs. SHEDs and SCAPs expressed typical levels of mesenchymal stem cell surface markers. CS-S was able to release the trophic factors in a sustained manner, but each factor has its own release kinetics. The CS-S group showed a significantly higher proliferation rate, accelerated the chemotaxis, and higher capacity to form vascular-like structures. CS-S provided a sustained and controlled release of trophic factors, which, in turn, improved proliferation, chemotaxis and all angiogenesis parameters in the co-culture. Thus, the functionalization of chitosan scaffolds loaded with secretomes is a promising platform for cell homing-based tissue engineering.

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Section: Discussionsupporting

confidence: 79%

Pro‐angiogenic potential of a functionalized hydrogel scaffold as a secretome delivery platform: An innovative strategy for cell homing‐based dental pulp tissue engineering

Carvalho

Sarra

Schröter

et al. 2022

J Tissue Eng Regen Med

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show abstract

“…However, a later study revealed that the molecules secreted by hDPSCs and SHEDs into the conditioned medium (CM) showed a bioactive function and regenerative ability [ 12 , 13 , 35 ]. As with BMMSCs, different proteins can be released by hDPSCs and SHEDs in the CM or inside the EVs [ 12 , 35 , 53 , 72 ].…”

Section: Secretome Of Dental Pulp Mscs and Their Biological Effectmentioning

confidence: 99%

“…Secreted neuroprotective factors may bind to specific receptors and trigger the activation of certain signalling pathways that coordinate cell function and survival [ 111 ]. In an in vitro model of PD, the SHED-CM demonstrated neuroprotective effects enhancing neurite outgrowth and repressed 6-hydroxydopamine-induced cell death [ 72 ]. Similarly, the SHED-CM showed a positive outcome in a PD rat model [ 108 , 112 , 113 ].…”

Section: Dscs Secretome In Central Nerve System Diseases Therapymentioning

confidence: 99%

Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Bär

Lis-Nawara

Grelewski

2021

IJMS

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The therapeutic potential of the dental pulp stem (DSC) cell-derived secretome, consisting of various biomolecules, is undergoing intense research. Despite promising in vitro and in vivo studies, most DSC secretome-based therapies have not been implemented in human medicine because the paracrine effect of the bioactive factors secreted by human dental pulp stem cells (hDPSCs) and human exfoliated deciduous teeth (SHEDs) is not completely understood. In this review, we outline the current data on the hDPSC- and SHED-derived secretome as a potential candidate in the regeneration of bone, cartilage, and nerve tissue. Published reports demonstrate that the dental MSC-derived secretome/conditional medium may be effective in treating neurodegenerative diseases, neural injuries, cartilage defects, and repairing bone by regulating neuroprotective, anti-inflammatory, antiapoptotic, and angiogenic processes through secretome paracrine mechanisms. Dental MSC-secretomes, similarly to the bone marrow MSC-secretome activate molecular and cellular mechanisms, which determine the effectiveness of cell-free therapy. Many reports emphasize that dental MSC-derived secretomes have potential application in tissue-regenerating therapy due to their multidirectional paracrine effect observed in the therapy of many different injured tissues.

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“…Ascorbic acid treatment of SHEDs increased the release of growth factors necessary for the tissue regeneration and homeostasis, including VEGF, SCF, IGF-1, HGF, bFGF, Ang-1, and EGF, and anti-inflammatory cytokines, such as NO, indoleamine 2,3-dioxygenase (IDO), PGE-2, IL-10, and IL-6. On the contrary, inflammatory cytokines CCL2 and TGF-β1 were reduced [ 51 ].…”

Section: Mscs Secretomementioning

confidence: 99%

Dental Mesenchymal Stem Cell Secretome: An Intriguing Approach for Neuroprotection and Neuroregeneration

Mazzon

2021

IJMS

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Mesenchymal stem cells (MSCs) are known for their beneficial effects and regenerative potential. In particular, dental-derived MSCs have the advantage of easier accessibility and a non-invasive isolation method. Moreover, thanks to their neural crest origin, dental MSCs seem to have a more prominent neuroregenerative potential. Indeed, in basal conditions they also express neuronal markers. However, it is now well known that the beneficial actions of MSCs depend, at least in part, on their secretome, referring to all the bioactive molecules released in the conditioned medium (CM) or in extracellular vesicles (EVs). In this review we focus on the applications of the secretome derived from dental MSCs for neuroregeneration and neuroprotection. The secretomes of different dental MSCs have been tested for their effects for neuroregenerative purposes, and the secretomes of dental pulp stem cells and stem cells from human exfoliated deciduous teeth are the most studied. Both the CM and EVs obtained from dental MSCs showed that they are able to promote neurite outgrowth and neuroprotective effects. Interestingly, dental-derived MSC secretome showed stronger neuroregenerative and neuroprotective effects compared to that obtained from other MSC sources. For these reasons, the secretome obtained from dental MSCs may represent a promising approach for neuroprotective treatments.

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Effect of Ascorbic Acid on Differentiation, Secretome and Stemness of Stem Cells from Human Exfoliated Deciduous Tooth (SHEDs)

Cited by 29 publications

References 62 publications

Pro‐angiogenic potential of a functionalized hydrogel scaffold as a secretome delivery platform: An innovative strategy for cell homing‐based dental pulp tissue engineering

Pro‐angiogenic potential of a functionalized hydrogel scaffold as a secretome delivery platform: An innovative strategy for cell homing‐based dental pulp tissue engineering

Dental Pulp Stem Cell-Derived Secretome and Its Regenerative Potential

Dental Mesenchymal Stem Cell Secretome: An Intriguing Approach for Neuroprotection and Neuroregeneration

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