Effect of ascorbic acid and citric acid on the quality of salted Chinese cabbage during storage

Chung, Young Bae; Song, Hye-Yeon; Jo, Kyungae; Suh, Hyung Joo

doi:10.1007/s10068-020-00857-w

Cited by 7 publications

(1 citation statement)

References 26 publications

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“…Citric acid is also used as a crosslinking agent, correcting pH and chelating metal ions in antioxidant systems [29]. Its application can serve as an inhibitor of kimchi browning [30]. It is worth noting that citric acid is also used as a crosslinking compound in the production of biodegradable plastics such as polyvinyl alcohol/starch [31], polyvinyl alcohol/xylan [32], starch films [33] and tragacanth gums [34].…”

Section: Introductionmentioning

confidence: 99%

Application of New Yarrowia lipolytica Transformants in Production of Citrates and Erythritol from Glycerol

Rywińska,

Tomaszewska-Hetman,

Lazar

et al. 2024

IJMS

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Citric acid and erythritol are obtained on an industrial scale using biotechnological methods. Due to the growing market demand for these products, research is underway to improve the process economics by introducing new microorganisms, in particular of the species Yarrowia lipolytica. The aim of this study was to evaluate transformants of Y. lipolytica for growth and ability to overproduce citric acids and erythritol from glycerol. The transformants were constructed by overexpressing glycerol kinase, methylcitrate synthase and mitochondrial succinate-fumarate transporter in the mutant Wratislavia 1.31. Next, strains were assessed for biosynthesis of citrate (pH 5.5; nitrogen limitation) and erythritol (pH 3.0; high osmotic pressure) from glycerol. Regardless of culture conditions strains, 1.31.GUT1/6 and 1.31.GUT1/6.CIT1/3 exhibited high rates of substrate utilization. Under conditions favoring citrate biosynthesis, both strains produced several percent more citrates, accompanied by higher erythritol production compared to the parental strain. During erythritol biosynthesis, the strain 1.31.GUT1/6.CIT1/3.E34672g obtained as a result of co-expression of all three genes stood out, producing 84.0 g/L of erythritol with yield and productivity of 0.54 g/g and 0.72 g/Lh, respectively, which places it in the group of the highest-ranked producers of erythritol among Y. lipolytica species.

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