Effect of Aromatic Compounds on Cellular Fatty Acid Composition of
            <i>Rhodococcus opacus</i>

Tsitko, Irina; Zaitsev, Gennadi; Lobanok, A G; Salkinoja‐Salonen, Mirja

doi:10.1128/aem.65.2.853-855.1999

Cited by 74 publications

(34 citation statements)

References 22 publications

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“…Aromatic solvents are known to be cytotoxic by partitioning into and disturbing the integrity of the cytoplasmic membrane [1,2]. This toxicity can be estimated by determining the partition coe¤cient (P OW ) of a solvent, which indicates how the compound will partition over a two phase octanol/water system.…”

Section: Introductionmentioning

confidence: 99%

The modification of the membrane of Oceanomonas baumanniiT when subjected to both osmotic and organic solvent stress

Brown

2000

FEMS Microbiology Letters

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Oceanomonas baumanniiT is a novel halotolerant bacterium which was isolated from the estuary of the river Wear (Sunderland, UK). When grown in tryptone soya broth it can tolerate high levels of phenol, which is not utilised as a carbon source in this medium. However, the level of tolerance was reduced from 35 mM to 3 mM phenol as salinity increased from 1% to 12% NaCl (w/v). Increasing salinity up to 12% NaCl also decreased the growth rate 8-fold and caused modifications to the cytoplasmic membrane particularly anionic phosphatidylglycerol levels, which doubled at the expense of zwitterionic phosphatidylethanolamine. In addition, changes in the phospholipid fatty acid composition were noted, cis-vaccenic acid decreased significantly at higher salinities. Intracellular solute levels also increased with increasing salinity and there was an accumulation of the compatible solutes ectoine, glycine betaine and glutamate. The addition of phenol to osmotically compromised cultures led to a further modification of the cytoplasmic membrane phospholipid composition, in particular, that the decrease in zwitterionic phosphatidylethanolamine and the increase of anionic phospholipid species was much less pronounced. A further decrease in unsaturation, particularly in the proportion of cis-vaccenic acid, and the mean chain length of the fatty acids suggested that this response was important in maintaining membrane integrity in the presence of phenol. ß

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Section: Introductionmentioning

confidence: 99%

The modification of the membrane of Oceanomonas baumanniiT when subjected to both osmotic and organic solvent stress

Brown

2000

FEMS Microbiology Letters

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“…Gonzalez et al (2001) have reported higher cell membrane rigidity due to the decrease in the ratio of cis/trans fatty acids following growth of P. putida DOT-T1E on higher concentrations of PHB. In Rhodococcus opacus GM-14 there is an increase in the content of 10-methyl branched fatty acids during its growth on aromatic compounds (Tsitko et al, 1999). The results obtained in our study show that A. protophormiae RKJ100 has only straight chain saturated fatty acids and alteration of the ratio of anteiso/iso fatty acids might affect the fluidity of membrane in response to lipophilic aromatic compounds.…”

Section: Resultsmentioning

confidence: 54%

“…Among Gram-positive bacteria, Rhodococcus opacus GM-14 is capable of growth at high concentrations of benzene, 4-chlorophenol and phenol (Zaitsev et al, 1995). Strain GM-14 exhibits altered cellular fatty acid composition when subjected to growth on the above aromatic compounds (Tsitko et al, 1999). The fatty acid analysis of A. protophormiae RKJ100 was carried out using cell mass obtained following its growth on 20 mM sodium succinate or separately on different concentrations of ONB and PHB.…”

Section: Resultsmentioning

confidence: 99%

Growth and physiological response of Arthrobacter protophormiae RKJ100 toward higher concentrations of o-nitrobenzoate and p-hydroxybenzoate

Sharma

Pandey

Gupta

et al. 2007

FEMS Microbiology Letters

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Bioremediation of sites that are heavily contaminated with pollutant chemicals is a challenge as most of the microorganisms cannot tolerate higher concentrations of toxic compounds. Only a few strains of the genus Pseudomonas have been studied for their tolerance toward the higher concentrations of aromatic pollutant compounds, a phenomenon that is accompanied by various physiological changes. In the present study we have characterized the growth response and physiological changes (adaptations) of a Gram-positive bacterium, Arthrobacter protophormiae RKJ100, toward the higher concentrations of two aromatic compounds, viz. o-nitrobenzoate (ONB) and p-hydroxybenzoate (PHB). Arthrobacter protophormiae RKJ100 could utilize 30 mM ONB and 50 mM PHB as sole sources of carbon and energy. It was capable of growth on higher concentrations of ONB (up to 200 mM) and PHB (up to 150 mM) when the cells were pre-exposed to lower concentrations of these compounds. The adaptive responses shown by the organism during growth on higher concentrations of these compounds were evident from significant changes in cellular fatty acid profiles. In addition, Bacterial Adhesion To Hydrocarbon (BATH) assay and scanning electron microscopy showed substantial increase in cell surface hydrophobicity and decrease in cell size of A. protophormiae RKJ100 when grown on ONB and PHB as compared to succinate-grown cells.

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“…A novel starvation biomarker: 10Me16:0 to 16:1v7c ratio Starvation C is a new biomarker proposed in the present work. It was recently reported [36] that Rhodococcus opacus markedly increased the proportion of midchain branched fatty acids when exposed to aromatic compounds, such as benzene or toluene. Cyclopropyl fatty acids are produced by Proteobacteria when challenged with solvent or when starved [31], which suggests that midchain branched fatty acids also may be used as a starvation biomarker.…”

Section: Interaction Of Metals With Dieselmentioning

confidence: 97%

EFfects of Diesel and Interactions with Copper and Other Metals in an Estuarine Sediment Microbial Community

Hedrick

Peacock²,

Tita

et al. 2009

Enviro Toxic and Chemistry

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Estuarine sediment microcosms were treated with combinations of diesel, copper (at two levels), and a mixture of heavy metals (mercury, cadmium, lead, and chromium; at two levels) mimicking the contaminant loadings found in harbor sediments. The effects on the microbial community were monitored by polar lipid fatty acid analysis. Diesel addition increased microbial biomass, caused shifts in some fatty acid structural groups, and decreased starvation biomarkers. Incorporation of diesel hydrocarbons into lipids was expressed as an increase in the proportion of odd-carbon-number fatty acids. No treatment with the metals mixture (mercury, cadmium, lead, and chromium) alone significantly changed any parameter derived from the polar lipid fatty acids, but the increase in microbial biomass from diesel addition was higher with the metals mixture, possibly because of indirect effects caused by reductions in grazing resulting from metal-induced toxicity to bacteriovorous nematodes. Copper also modified the effects of diesel addition, preventing biomass increase but not diesel degradation, suggesting that some of the energy gained from diesel oxidation was expended combating copper toxicity. In the present study, observations indicate that metals in general, and copper in particular, can modify the response of sedimentary microorganisms to petroleum-hydrocarbon contaminants.

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Effect of Aromatic Compounds on Cellular Fatty Acid Composition of Rhodococcus opacus

Cited by 74 publications

References 22 publications

The modification of the membrane of Oceanomonas baumanniiT when subjected to both osmotic and organic solvent stress

The modification of the membrane of Oceanomonas baumanniiT when subjected to both osmotic and organic solvent stress

Growth and physiological response of Arthrobacter protophormiae RKJ100 toward higher concentrations of o-nitrobenzoate and p-hydroxybenzoate

EFfects of Diesel and Interactions with Copper and Other Metals in an Estuarine Sediment Microbial Community

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