Effect of Aroclor 1254 on Sertoli cellular antioxidant system, androgen binding protein and lactate in adult rat in vitro

Giribabu, K.; Parthasarathy, M.; Muthuvel, R.; Gunadharini, Dharmalingam Nandagopal; Vijayababu, M.R.; Arumugam, Arunkumar; Venkataraman, P.; Aruldhas, M. M.; Arunakaran, J.

doi:10.1016/j.tox.2005.04.014

Cited by 43 publications

(23 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This effect may be accomplished through their production of various factors such as plasminogen activator, transferrin, and sertoli cell growth factor Feig et al, 1980;Skinner and Griswold, 1980). Besides, sertoli cells can maintain high concentrations of androgens in seminiferous tubules and epididymis , they can also transport testosterone from the testis into epididymis (Krishnamoorthy et al, 2005). Our research showed supplementing VE in diet increased the density of sertoli cell, which in Group 2, Group 3 and Group 4 was significantly higher than in Control respectively (P<0.05) and Group 2 was the highest.…”

Section: Discussionmentioning

confidence: 99%

Effect of Vitamin E on the Development of Testis in Sheep

Luo¹,

Ge²,

Yue³

et al. 2011

Artificial Insemination in Farm Animals

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Effect of Vitamin E on the Development of Testis in Sheep

Luo¹,

Ge²,

Yue³

et al. 2011

Artificial Insemination in Farm Animals

View full text Add to dashboard Cite

“…A continuous exposure of lactating mothers to Aroclor 1242 causes significant effects on Leydig cell structure and function, hypotrophy, and reduced capacity to produce testosterone in vitro in response to LH stimulation (Kim et al 2001). Recent studies from our laboratory have demonstrated that adult rats exposed to PCBs disrupt Sertoli and Leydig cellular functions in adult rats (Senthil kumar et al 2004, Krishnamoorthy et al 2005, Murugesan et al 2005b. The cultured Leydig cells from adult rats that were exposed to PCBs resulted in lowered synthesis of testosterone following human chorionic gonadotropin stimulation (Kovacevic et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Effects of polychlorinated biphenyl (Aroclor 1254) on steroidogenesis and antioxidant system in cultured adult rat Leydig cells

Palaniappan¹,

Balaganesh²,

Balasubramanian³

et al. 2007

Journal of Endocrinology

View full text Add to dashboard Cite

Polychlorinated biphenyls (PCBs) are ubiquitous and persistent environmental contaminants that disturb normal endocrine functions, including gonadal functions in humans and mammals. In the present study, we examined the direct effects of PCB on rat Leydig cells in vitro. Adult Leydig cells were purified by Percoll gradient centrifugation method and the purity of Leydig cells was also determined by 3b-hydroxysteroid dehydrogenase (3b-HSD) staining method. Purified Leydig cells were exposed to different concentrations (10 K10-10 K7 M) of PCB (Aroclor 1254) for 24 h under basal and LH-stimulated conditions. After the experimental period, cultured media were collected and used for the assay of testosterone and estradiol. The treated cells were used for the quantification of cell-surface LH receptors and activities of steroidogenic enzymes, such as cytochrome P 450 side-chain cleavage enzyme (P 450 scc), 3b-HSD, and 17b-hydroxysteroid dehydrogenase (17b-HSD). Leydig cellular enzymatic antioxidants, such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, g-glutamyl transpeptidase, glutathione-S-transferase, and nonenzymatic antioxidants, such as vitamins C and E, were assayed. Lipid peroxidation (LPO) and reactive oxygen species (ROS) were also estimated in Leydig cells. In addition, total RNA was isolated from control and Aroclor 1254-exposed Leydig cells to monitor the steady-state mRNA levels by reverse transcription(RT)-PCR for steroidogenic acuteregulatory (StAR) protein, cytochrome P 450 scc, 3b-HSD, and 17b-HSD. Our results indicated that Aroclor 1254 (10 K9 , 10 K8 , and 10 K7 M) treatments significantly inhibit basal and LH-stimulated testosterone and estradiol production. In addition, the activities of steroidogenic enzymes, enzymatic and nonenzymatic antioxidants were significantly diminished in a dose-dependent manner. However, LPO and ROS were elevated in a dose-dependent manner under basal and LH-stimulated conditions. RT-PCR analysis of StAR mRNA level showed a decrease only in 10 K7 M dose of Aroclor 1254 treatment, while cytochrome P 450 scc, 3b-HSD, and 17b-HSD mRNAs were drastically decreased in both 10 K8 and 10 K7 M Aroclor 1254 treatment. These findings suggest that PCBs can act directly on Leydig cells to diminish testosterone production by inhibiting gene expression of steroidogenic enzymes and antioxidant system.

show abstract

“…SOD is considered as the first line of defense against deleterious effects of oxyradicals in the cell by catalyzing the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. [29] The decreased activity of CAT in the testes of aluminum chloride treated animals observed in the present study may reflect the inability of these organs to eliminate hydrogen peroxide produced by the influence of aluminum chloride.…”

Section: Discussionmentioning

confidence: 50%

Antioxidant Effect of Ethanolic Leaf Extract of Piper Guineense in Aluminium Chloride Induced Reproductive Toxicity and Oxidative Stress in Male Albino Rats.

Arhoghro¹

2017

WJPPS

View full text Add to dashboard Cite

Aluminium is one of the highly abundant element in the environment and the and the third most common metal in the earth crust. Piper guinense is herbal medicine used for the treatment of many diseases like infertility. This study shows the antioxidant effect of Piper guineense on infertility caused by aluminium chloride in male wistar rats. Male wistar albino rats weighing between 150-200g was randomly separated into five different groups. The first group served as negative control and received distilled water and standard feed. Group two served as positive control and received AlCl 3 (4.3mg/kg bw) and Group three received AlCl 3 (4.3mg/kg bw) and Piper guineense (100mg/kg bw) for 21 days.There was a significant (P<0.05) decrease in the activities of SOD, CAT, GPX, GST, GSH and there was a significant (P<0.05) increase in LPO from, in rats treated with AlCl 3 (4.3mg/kg bw) compared to the negative control.Also there was a significant (P<0.05) increase in the activities of SOD , CAT, GPX, GST and GSH in rat testis treated with Piper guineense (100mg/kg bw) plus AlCl 3 (4.3mg/kg bw) compared to the positive control while there was a significant (P<0.05) decrease in LPO from12.75 ± 0.521to 9.02 ± 0.439 in rat's testis treated with Piper guineense (100mg/kg bw) plus AlCl 3 (4.3mg/kg bw) compared to the positive control. There was a significant (P<0.05) increase in activities of ALT and AST, in rat serum treated with AlCl 3 (4.3mg/kg bw) compared to the negative control and there was a

show abstract

Effect of Aroclor 1254 on Sertoli cellular antioxidant system, androgen binding protein and lactate in adult rat in vitro

Cited by 43 publications

References 59 publications

Effect of Vitamin E on the Development of Testis in Sheep

Effect of Vitamin E on the Development of Testis in Sheep

Effects of polychlorinated biphenyl (Aroclor 1254) on steroidogenesis and antioxidant system in cultured adult rat Leydig cells

Antioxidant Effect of Ethanolic Leaf Extract of Piper Guineense in Aluminium Chloride Induced Reproductive Toxicity and Oxidative Stress in Male Albino Rats.

Contact Info

Product

Resources

About