Effect of argon‐plasma treatment on proliferation of human‐skin–derived fibroblast on chitosan membrane <i>in vitro</i>

Zhu, Xin; Chian, Kerm Sin; Chan‐Park, Mary B.; Lee, Seng Teik

doi:10.1002/jbm.a.30211

Cited by 90 publications

(54 citation statements)

References 56 publications

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“…The few viable cells adhered on the CTS sample surface after 10 days of culture did not present the typical fibroblast-like morphology, being quite round and sparsely distributed. The poor cell adhesion and proliferation on chitosan membranes have been previously reported (Lopez-Perez et al, 2007;Zhu et al, 2005). The iPrOH50 membranes presented very similar results (not shown).…”

Section: Cell Sheet Detachment and Assessment Of The Cell Viabilitysupporting

confidence: 90%

Poly(N‐isopropylacrylamide) surface‐grafted chitosan membranes as a new substrate for cell sheet engineering and manipulation

Silva

López-Pérez

Elvira

et al. 2008

Biotech & Bioengineering

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The immobilization of poly(N-isopropylacrylamide) (PNIPAAm) on chitosan membranes was performed in order to render membranes with thermo-responsive surface properties. The aim was to create membranes suitable for cell culture and in which confluent cell sheets can be recovered by simply lowering the temperature. The chitosan membranes were immersed in a solution of the monomer that was polymerized via radical initiation. The composition of the polymerization reaction solvent, which was a mixture of a chitosan non-solvent (isopropanol) and a solvent (water), provided a tight control over the chitosan membranes swelling capability. The different swelling ratio, obtained at different solvent composition of the reaction mixture, drives simultaneously the monomer solubility and diffusion into the polymeric matrix, the polymerization reaction rate, as well as the eventual chain transfer to the side substituents of the pyranosyl groups of chitosan. A combined analysis of the modified membranes chemistry by proton nuclear magnetic resonance ((1)H-NMR), Fourier transform spectroscopy with attenuated total reflection (FTIR-ATR) and X-ray photoelectron spectroscopy (XPS) showed that it was possible to control the chitosan modification yield and depth in the solvent composition range between 75% and 100% of isopropanol. Plasma treatment was also applied to the original chitosan membranes in order to improve cell adhesion and proliferation. Chitosan membranes, which had been previously subjected to oxygen plasma treatment, were then modified by means of the previously described methodology. A human fetal lung fibroblast cell line was cultured until confluence on the plasma-treated thermo-responsive chitosan membranes and cell sheets were harvested lowering the temperature.

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Section: Cell Sheet Detachment and Assessment Of The Cell Viabilitysupporting

confidence: 90%

Poly(N‐isopropylacrylamide) surface‐grafted chitosan membranes as a new substrate for cell sheet engineering and manipulation

Silva

López-Pérez

Elvira

et al. 2008

Biotech & Bioengineering

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“…Solid freeform fabrication process is a computerized technique involving the design of a scaffold model through a CAD system which elaborates it as a series of cross sections [84,[101][102][103]. These sections are built by a rapid prototyping machine that lays down layers of material starting from the bottom and moving up a layer at a time to create the scaffold.…”

Section: Solid Freeform Fabricationmentioning

confidence: 99%

“…(i) plasma modification: a proper selection of the plasma source enables to modify wettability and functional groups at the biomaterial surface in order to improve cell adhesion [103][104][105];…”

Section: Surface Functionalizationmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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“…After gas plasma treatment, bone marrow stromal cells do attach to PEGT/PBT copolymers, enabling in vitro bone marrow stromal cell culturing and making bone tissue engineering applications of these materials possible [6] . Further studies demonstrated that the gas plasma treatment improved the surface hydrophilicity of chitosan membranes and promoted attachment and proliferation of human-skin-derived fibroblasts [7] and the growth of human endothelial cells on plasma-treated polyethylene terephthalate and polytetrafluoroethylene (PTFE) surfaces [8] . An enhanced cell affinity of poly( D , L -lactide) could be demonstrated by combining plasma treatment with collagen coating [9] .…”

mentioning

confidence: 99%

Improved Neovascularization of PEGT/PBT Copolymer Matrices in Response to Surface Modification by Biomimetic Coating

Ring¹,

Steinstraesser

Muhr³

et al. 2007

Eur Surg Res

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PEGT/PBT (polyethylene glycol terephthalate/polybutylene terephthalate) copolymer matrices with three different surface coatings [calcium-phosphate (Ca-P), collagen, and gas plasma] were placed into dorsal skinfold chambers of 24 balb/c mice. Untreated PEGT/PBT matrices served as the controls. The basal surfaces of the implants directly contacted the striated skin muscle. Neovascularization of the implants was analyzed by intravital fluorescence microscopy. Microcirculatory observations were performed in the surrounding skin muscle, at the border zone of the implant, and in the center of the implant. The functional vessel density (FVD; mm/mm²), as the length of perfused microvessels per observation area, was measured by computer-assisted analysis. The FVD served as the parameter of neovascularization. At the end of the protocol, histological observation of hematoxylin/eosin-standard-stained sections was performed by light microscopy. The FVD in the center of the implant on day 8 was only observed in gas-plasma-coated (8.8 ± 10.2 mm/mm²) and Ca-P-coated implants (0.8 ± 2.0 mm/mm²). None of the other groups showed perfused microvessels in the center of the implant on day 8 (p < 0.05). The FVD values in the center of the gas-plasma-coated and the Ca-P-coated implants were 20.7 ± 8.2 and 19.2 ± 15.5 mm/mm² as compared with 7.1 ± 17.4 and 7.7 ± 5.9 mm/mm² for collagen-coated and untreated implants on day 16. The histological examination confirmed the profound microvascular ingrowth into the matrix pores of the gas-plasma-treated and the Ca-P-coated copolymer matrices in the center of the implants. The study showed that the ingrowth of microvessels into PEGT/PBT matrices can be accelerated by Ca-P coating and gas plasma treatment in the dorsal skinfold chamber in mice.

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Effect of argon‐plasma treatment on proliferation of human‐skin–derived fibroblast on chitosan membrane in vitro

Cited by 90 publications

References 56 publications

Poly(N‐isopropylacrylamide) surface‐grafted chitosan membranes as a new substrate for cell sheet engineering and manipulation

Poly(N‐isopropylacrylamide) surface‐grafted chitosan membranes as a new substrate for cell sheet engineering and manipulation

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Improved Neovascularization of PEGT/PBT Copolymer Matrices in Response to Surface Modification by Biomimetic Coating

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