Effect of arginine-vasopressin on the rat thyroid gland in vitro

Dityateva, G. V.; Krasnovskaya, I. A.; Skopicheva, V. I.

doi:10.1007/bf00842306

Cited by 2 publications

(2 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The existence of such connections is confirmed by the thyreostimulating effect of vasopressin and oxytocin [2][3][4] and by the effect of TRH on the functional state of PVN and SON cells in vivo [1,9]. However, the data obtained in vivo provide little information on the mechanism of TRH action on nonapeptidergic cells.…”

Section: Abstract: Thyroliberin; Vasopressin; Oxytocin; Hypothalamusmentioning

confidence: 62%

Effect of thyroliberin on nonapeptidergic cells in cultured rat hypothalamic slices

Глазова

Krasnovskaya

1999

Bull Exp Biol Med

Self Cite

View full text Add to dashboard Cite

The direct effect of thyroliberin on nonapeptidergic cells of the supraoptic and paraventricular nuclei was studied on cultured rat hypothalamic slices. Incorporation of labeled thyroliberin into vasopressinergic and oxytocinergic cells and a decrease in functional activity of these cells were observed in both hypothalamic nuclei. Key Words: thyroliberin; vasopressin; oxytocin; hypothalamus; paraventricular nucleus; supraoptic nucleusMost peptide hypothalamic hormones act as neurohormones affecting the target cells via blood and liquor and as neurotransmitters, i.e. affect other nerve cells (including those producing neurohormones) via the corresponding synapses [7]. There is evidence on the presence of corticoliberinergic synapses of cells of the paraventricular hypothalamic nucleus (PVN) that produce thyroliberin (TRH) [12]. Somatostatin-and somatoliberin-producing cells of the arcuate nucleus also interact via synaptic connections [11 ].The interaction between the cells producing releasing-hormones, in particular between TRH-cells and nonapeptidergic cells in the hypothalamic PVN and supraoptic nucleus (SON) remains unclear. The existence of such connections is confirmed by the thyreostimulating effect of vasopressin and oxytocin [2][3][4] and by the effect of TRH on the functional state of PVN and SON cells in vivo [1,9]. However, the data obtained in vivo provide little information on the mechanism of TRH action on nonapeptidergic cells. Our aim was to carry out a morphofunctional study of vasopressin-and oxytocinergic cells in isolated hypothalamic slices exposed to TRH. MATERIALS AND METHODSThe study was carried out on mature male Wistar rats weighing 120-140 g kept under standard vivarium Laboratory-of Neuroendocrinology, I. M. Sechenov Institute of Evolution, Physiology, and Biochemistry, Russian Academy of Sciences, St. Petersbur 9 conditions. After decapitation, the brain was rapidly removed and 400-p slices contaimng SON and/or PVN were prepared from the hypothalamic area. The slices were preincubated in Earle's medium aerated with carbogen (95% 02+5% CO2) at 37~ Preincubation was performed in 3 portions of the growth medium (30 min in each portion).In the first experimental series we studied incorporation of 3H-TRH into neurosecretory cells of PVN and SON. After preincubation, the hypothalamic slices were transferred to the growth medium with 3H-TRH (1.75• 1.85x106 Bq/mol, NEN Product) and incubated for 15 and 30 min (the medium was changed every 15 min).The slices were fixed in picric acid and formalin (3:5) and processed by standard histological methods. Serial sections (6 p) were used in immunohistochemical PAP-reaction pertormed with nonlabeled antibodies to oxytocin. The sections were covered with an M-type photoemulsion (KhimFotoProekt, Moscow), exposed for 6 weeks, and developed in 2,4-diaminophenol dihydrochloride. Binding of 3H-TRH in PVN and SON cells was accesses by the number of silver grains concentrated above one cell (30 vasopressin-and 30 oxytocinergic cells for each rat). The number ...

show abstract

Section: Abstract: Thyroliberin; Vasopressin; Oxytocin; Hypothalamusmentioning

confidence: 62%

Effect of thyroliberin on nonapeptidergic cells in cultured rat hypothalamic slices

Глазова

Krasnovskaya

1999

Bull Exp Biol Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…The direct effect of the hypothalamic hormones vasopressin (VP) and oxytocin (OT) on functional activity of the thyroid gland is well established [1,2]. However, the possibility of feed-back regulation of VP-and OT-cells of the hypothalamus by thyroid hormones (TH) remains unsolved.…”

Section: Abstract: Thyroid Hormones; Vasopressin; Oxytocin; Paraventmentioning

confidence: 99%

Effect of thyroid hormones on neurosecretory cells of supraoptic and paraventricular nuclei of rat hypothalamusin vitro

et al. 1997

Self Cite

View full text Add to dashboard Cite

The direct effect of thyroid hormones on oxytocin-and vasopressinergic cells is studied in vitro on cultured slices of rat hypothalamus containing the paraventricular and/or supraoptic nuclei. It is shown that 30-and 60-min exposure to thyroxine leads to considerable shrinkage of the nucleoli of oxytocin-and vasopressin-secreting cells in the supraoptic and paraventricular nuclei, which reflects the decrease in their functional activity. The addition of triiodothyronine leads to stimulation of neurosecretory cells (30-min incubation) followed by a drop in their functional activity. Key Words: thyroid hormones; vasopressin; oxytocin; paraventricular nucleus; supraoptic nucleusThe direct effect of the hypothalamic hormones vasopressin (VP) and oxytocin (OT) on functional activity of the thyroid gland is well established [1,2]. However, the possibility of feed-back regulation of VP-and OT-cells of the hypothalamus by thyroid hormones (TH) remains unsolved. TH receptors were identified in many areas of the brain, including the hypothalamus [7]. Conceivable TH influence the VP cells since different thyroid statuses are accompanied by shifts in the blood VP level [5,6]. However, under conditions of long-term hormone imbalance it is very difficult to determine which factor plays a key role in the regulation of endocrine organs. It has been shown that TH participate in the regulation of the OT gene in vitro [3,4].In the present study we examined the effect of short-term direct exposure of cells of paraventricular and supraoptic nuclei to TH under conditions of in vitro incubation of hypothalamic sections in the presence of TH. MATERIALS AND METHODSSections of the hypothalamus were obtained from male Wistar rats (120-140 g). After decapitation, the brain was promptly removed and 400-g sections containing supraoptic and/or paraventricular nuclei were prepared from the hypothalamic area. The sections were preincubated in Eafle's medium saturated with carbogen (95% 02+5% CO2) at 37~ for 90 min. The medium was replaced every 30 min.Two experimental series were carried out. In both series binding of triiodothyronine (T3) and thyroxine (T4) was measured using radiolabeled hormones: 125I-T3 and I-%I-T4.After preincubation, the sections were transferred to a medium containing 2.7x 10 .9 M ~zSI-T3 (74x 10 js Bq/mol, series I) or 4.5x 10 .9 M ~25I-T 4 (140x 10 ~5 Bq/mol, series II). Control sections were incubated without TH. After 30-and 60-min incubation, the sections were fixed in picric acid and formalin (3:5) at 37~ for 7 days and processed by standard histological methods. Histological sections (6 ~) were stained with paraldehyde-fuchsin by the Gomori--Gabu method and poststained with azan by the Heidenhain method.

show abstract

Effect of arginine-vasopressin on the rat thyroid gland in vitro

Cited by 2 publications

References 10 publications

Effect of thyroliberin on nonapeptidergic cells in cultured rat hypothalamic slices

Effect of thyroliberin on nonapeptidergic cells in cultured rat hypothalamic slices

Effect of thyroid hormones on neurosecretory cells of supraoptic and paraventricular nuclei of rat hypothalamusin vitro

Contact Info

Product

Resources

About