Effect of Aqueous Ozone Solution Irrigation on Healing after Treatment with Dental Implants: A Cross-over Randomized Controlled Clinical Trial

Abstract

The aim of this study was to determine the effect of topical irrigation with a solution of ozone (O 3 ) water on soft tissue healing after dental implantation. Recruited patients underwent similar oral surgeries (dental implantation) on both sides of their upper jaws. One randomly selected site was irrigated with 10 ml of O 3 water solution immediately following surgery and after 3 days. The other side was irrigated with normal saline. Wound healing was assessed 24 hours and 5 days following the surgery. Early healing scores (EHSs) were registered. Post-operative instructions were provided to all patients. Of them, 12 were male and 16 were female. Tissues at the sites of surgery were thin in 13 patients and thick in 15 patients. The proportions of sites irrigated with ozonated water that had merged incision margins, no fibrin at the incision margins, and no signs of inflammation were higher than those of sites irrigated with saline on days 1 and 5 following the surgery. On day 1, the median total EHSs for re-epithelization, hemostasis, and inflammation were significantly higher for intervention sites than those for control sites. However, no significant differences were observed in the studied parameters between the intervention and control sites on day 5. O 3 treatment enhances soft tissue healing in the immediate postoperative period. The assessment of long-term clinical outcomes of O 3 treatment should be investigated further.

Liquid ozone therapies for the treatment of epithelial wounds: A systematic review and meta‐analysis

“…Two studies on human subjects reported clinical signs of wound healing by treatment type. 34 , 40 (Table 4 ) Evaluating the efficacy of ozonated olive oil to treat diabetic foot ulcers, significantly fewer abnormal findings of ulcer wound healing (ie, absence of healing epithelial edges, unchanged areas of wound, moist granulation tissue, clinical signs of wound infection/changes of colour or amount of wound exudates) were found in the ozonated oil group than the control group at week 1 ( P < 0.001), week 3 ( P < 0.006) and week 5 ( P < 0.001). 34 In a study of oral wounds post dental implant surgery, compared to control wounds, wounds irrigated with ozonated water evidenced greater scores on clinical signs measuring re‐epithelization, decreased haemostasis, reduced inflammation, and overall early wound healing at days 1 and 5 ( P < 0.05).…”

“…34 In a study of oral wounds post dental implant surgery, compared to control wounds, wounds irrigated with ozonated water evidenced greater scores on clinical signs measuring re‐epithelization, decreased haemostasis, reduced inflammation, and overall early wound healing at days 1 and 5 ( P < 0.05). 40 …”

“…The remaining 13 studies had a mixture of low risk and unclear risk ratings. 19 , 21 , 24 , 25 , 26 , 27 , 29 , 30 , 33 , 35 , 36 , 37 , 40 …”

“…Two studies on human subjects reported clinical signs of wound healing by treatment type 34,40 . (Table 4) Evaluating the efficacy of ozonated olive oil to treat diabetic foot ulcers, significantly fewer abnormal findings of ulcer wound healing (ie, absence of healing epithelial edges, unchanged areas of wound, moist granulation tissue, clinical signs of wound infection/changes of colour or amount of wound exudates) were found in the ozonated oil group than the control group at week 1 ( P < 0.001), week 3 ( P < 0.006) and week 5 ( P < 0.001) 34 .…”

“…Three studies had high bias in blinding of participants and personnel, three had high risk in the blinding of outcome assessors, and four had other types of risk for high bias. The remaining 13 studies had a mixture of low risk and unclear risk ratings 19,21,24‐27,29,30,33,35‐37,40 …”