Effect of Aqueous Extract of Phenolic Compounds Obtained from Red Wine in Experimental Model of Colitis in Mice

Abstract

Background: Inflammatory bowel disease (IBD) is a chronic relapsing inflammatory disorder represented by Crohn’s disease and ulcerative colitis. Currently, there is no cure and pharmacological treatment aims to induce and maintain remission on patients. Because the therapy reveals a relatively high toxicity, during a long-term utilization, it is essential to investigate new pharmacological approaches. Polyphenols, commonly present on red wine, have shown health-beneficial effects related to their antioxidant and anti-inflammatory effects through the inhibition of NF-kB activation, COX-2 and iNOS induction. In this sense, it would be interesting to study their effects in an IBD context. Therefore, this study aims to evaluate the effects of an aqueous extract of phenolic compounds in a 2,4,6-Trinitrobenzenesulfonic acid (TNBS)-induced model of colitis. Method: Experimental colitis was induced in mice through an intrarectal administration of TNBS and then the mice were treated with an aqueous extract of phenolic compounds intraperitoneally for four days. Results and Discussion: The extract demonstrated an anti-inflammatory effect, reducing TNF-α levels in the colon, and had a beneficial effect on the extraintestinal manifestations related to IBD, without any significant side effects. The extract of phenolic compounds demonstrated to be a valuable object of study for the management of IBD in the future.

Hemin Ameliorates the Inflammatory Activity in the Inflammatory Bowel Disease: A Non-Clinical Study in Rodents

“…First, on day 0 (induction day), the mice were anesthetized with 40 μL of a mixture of ketamine 100 mg/kg + xylazine 10 mg/kg by an intraperitoneal (IP) injection. TNBS was instilled through a single intrarectal (IR) administration [ 16 , 20 , 21 , 22 , 23 ]. The technique consisted of the insertion of a cannula into the colon, such that the tip was 4 cm proximal to the anus, and then 100 μL of TNBS 1.0% was administrated over five weeks.…”

The Role of Anthocyanins in Alleviating Intestinal Diseases: A Mini Review

“…IBD is an immune-mediated chronic recurrent inflammatory disease characterized by the alternation of acute and remission phases, ,, with complex etiology and pathophysiology. According to forecasts, around seven million people are currently living with IBD .…”

“…10 Presently, millions of people all over the world suffer from intestinal diseases, including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and colorectal cancer (CRC). 7,8,11 However, the objective of managing IBD is to minimize distressing symptoms in order to achieve sustained remission, instead of curing it completedly. 12,13 CRC patients have experienced significant adverse effects from chemotherapy or radiotherapy.…”

“…The intestinal tract, known as “the second brain”, is the largest digestive and absorption organ in the body, colonized with many microorganisms that come into direct contact with nutrients, therapeutic drugs, and toxic food contaminants. , Thus, the intestine is not only vital for absorbing nutrition but also a major barrier against pathogenic microorganisms, and pathologies of the intestinal tract are closely related to the collapse of the defense system . Presently, millions of people all over the world suffer from intestinal diseases, including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS), and colorectal cancer (CRC). ,, However, the objective of managing IBD is to minimize distressing symptoms in order to achieve sustained remission, instead of curing it completedly. , CRC patients have experienced significant adverse effects from chemotherapy or radiotherapy . In addition, the pathogenesis of IBS is still unclear, and the treatment for IBS is currently limited to symptomatic management .…”

“…Colorful fruits, vegetables, flowers, and grains contain water-soluble pigments known as anthocyanins, which have shown bioactive properties in a number of ways. − As dietary supplements, anthocyanins provide a wide range of health benefits, for example, antioxidant, antimicrobial, anti-inflammatory, and neuroprotective. − The retrieval has already found that with the increasing number of publications, the renewed public interest of anthocyanins research is continuing, particularly in intestinal diseases. ,, …”