This study aims to determine the effect of combinations of fountain clerodendrum and avocado seed flour enhanced with nanotechnology on growth performance, intestinal profile, and microbial population of Lohman chickens. A total 256 Lohmann chicken were used on the experimental diets. The Fountain clerodendrum and avocado seed flour were enhanced with nanotechnology 500 mm. The two-way nested Anova with eight treatments and four replication was employed, with each experimental unit consisting of eight Lohmann broiler chickens. The treatments consisted of B 1 T 0 = basal diet, B 1 T 1 = basal diet + 0.4% avocado seed flour, B 1 T 2 = basal diet + 0.8% avocado seed flour, B 1 T 3 = basal diet + 1.2% avocado seed flour, B 2 T 0 = basal diet, B 2 T 1 = basal diet + 0.4% avocado seed flour enhanced with nanotechnology, B 2 T 2 = basal diet + 0.8% avocado seed flour enhanced with nanotechnology, B 2 T 3 = basal diet + 1.2% avocado seed flour enhanced with nanotechnology. If there is significant effect (p < 0.05), the analysis continued with least significant differences (LSD). The result showed significantly difference (p < 0.01) on villus height and significant difference (p < 0.05) on microbial population. In contrast, the result showed no significantly difference (p > 0.05). In conclusion, combinations of nano-fountain clerodendrum and avocado seed flour help to increasing villus height and adverse microbial on Lohmann broiler chickens.