Effect of Aqueous Extract of Phaseolus Vulgaris on the Regulation of Glucose and Lipid Metabolism in Diabetic Rats

Atchibri, Louise Anin Anin; Soro, Lêniféré Chantal; Diagou, Jean Jacques; Gnakri, D.

doi:10.9790/264x-0301014349

Cited by 1 publication

(2 citation statements)

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“…Researches have also shown that a combination of the freeze-dried seeds (300 mg/ kg) and glibenclamide (0.20 g/kg) resulted in an enhanced antihyperglycemic activities (Ocho-Anin Atchibri et al, 2010). Another study concluded that the aqueous extract of P. vulgaris seeds reduced blood glucose and restored normal insulin levels in diabetic rats (Atchibri et al, 2017). Additionally, red kidney beans of P. vulgaris showed in vitro anti-diabetic activity by inhibiting α-glucosidase enzyme (Lindawati et al, 2021).…”

Section: Introductionmentioning

confidence: 97%

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Non‐polar metabolites of green beans ( Phaseolus vulgaris L.) potentiate the antidiabetic activity of mesenchymal stem cells in streptozotocin‐induced diabetes in rats

Ezzat

Rahman

Salama

et al. 2022

Journal of Food Biochemistry

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Green beans (Phaseolus vulgaris L.) are consumed as pods or mature seeds (common beans). The pods were extracted with 95% ethanol and processed to prepare nonpolar and polar fractions. Comparing the antihyperglycemic activity of both fractions, non-polar fraction (NPF, 200 mg kg −1 day −1 ) lowered blood glucose in streptozotocin diabetic rats by 65% compared to 57% for the polar fraction at the same dose. When NPF treatment was combined with injection of mesenchymal stem cells (MSC) a 4.4fold increase in serum insulin and a 73.6% reduction in blood glucose were observed compared to untreated control. Additionally, a significant decrease in malondialdehyde (76.2%), nitric oxide (68.2%), cholesterol (76.1%), and triglycerides (69.5%) and a 1.75-fold increase in HDL concentrations were observed in the group treated with this combination compared to diabetic animals. Interestingly, NPF increased homing of MSC in pancreas potentiating their antidiabetic activity. Finally, 26 compounds were identified in NPF using LC/MS analysis and four were isolated in pure form. The isolated compounds namely calotroproceryl acetate, fridelin, calotroproceryl A, and stigmasterol showed good inhibitory activity against pancreatic lipase with IC 50 at 1.93, 1.07, 1.34 and 1.44-1 μg/ml, respectively. Additionally, these compounds inhibited α-amylase, albeit at higher concentration, with IC 50 at 248, 212, 254, and 155 μg/ ml for calotroproceryl acetate, fridelin, calotroproceryl A, and stigmasterol, respectively. Our results suggest that green beans extract can potentiate effect of MSC in diabetes directly due to its own antidiabetic effect and indirectly by increasing MSC homing in pancreatic tissues. Practical applicationsIt has been suggested in this study that green beans can improve hyperglycemia, oxidative balance in diabetes, so green beans can be promoted as a healthy nutrient for diabetic patients. Green beans also can enhance homing and differentiation of mesnchymal stem cells in the pancreas for future stem cell therapy of type I diabetes.

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Section: Introductionmentioning

confidence: 97%

“…Fabaceae (common beans, green beans, or French beans) is rich in bioactive constituents with beneficial impact on health (Atchibri et al, 2017;Ocho-Anin Atchibri et al, 2010). Aqueous extract of the pericarps of P. vulgaris (green beans) was found to improve metabolic regulation of diabetes in rats (Halenova et al, 2019).…”

Section: Introductionmentioning

confidence: 99%