Effect of Aqueous Extract and Fractions of Fagonia arabica on In Vitro Anticoagulant Activity

Abstract

Fagonia arabica (FA) is a deobstruent and blood purifier, which possesses thrombolytic and antioxidant activities. In this study, the anticoagulant effects of FA and its derived fractions were evaluated. Plasma recalcification was performed with multisolvent extracts of FA and then with extracts prepared successively with increasing polarity of the solvents. Aqueous extract was the most potent anticoagulant extract, which was fractionated by thin-layer chromatography and column chromatography. Five fractions collected were checked for their anticoagulation effect. The most potent fraction was screened for phytoconstituents. Aqueous extract of FA is the most active anticoagulant (31 minutes). Results were statistically significant when compared to heparin (38 minutes) and saline (4.04 minutes; P > .001). The Fifth fraction (FA5), the most potent fraction (27 minutes), was found positive for flavonoids, saponin, tannin, triterpenoids, carbohydrates, reducing sugar, and monosaccharides. Aqueous FA and fraction FA5 were most active in in vitro anticoagulation, and any of the phytochemicals identified could be considered the active component.

In-Vitro Anticoagulant Effect of Aerial Parts Extracts of Yemeni Fagonia Schweinfurthii Hadidi

“…These results agreed with Ismail et al,14 and Chourasia et al,15 that Fagonia cretica leaves extract and aqueous extract of Fagonia arabica and its fractions exhibited a concentrationdependent anticoagulant activity. A comparison of the effect of the three extracts on the PT and PTT showed that the ethyl acetate and methanol extract exhibited the highest anticoagulant action.…”

“…Phytochemical screening revealed that F. schweinfurthii extracts contain alkaloids, cardiac glycolsides, flavonoids, carbohydrates, tannins, saponins, steroids, and amino acids 5 . The anticoagulant activity of Fagonia arabica extracts (water, ethanol, methanol, isopropyl alcohol (IPA), butanol, ethyl acetate, acetone, chloroform, petroleum ether, and hexane) was studied in vitro and revealed that the aqueous extract and its fifth fraction had the greatest anticoagulant effect (31 minutes and 27 minutes, respectively) 15 . Furthermore, Ismail et al, 14 demonstrated that Fagonia cretica had marked anticoagulant activity with a coagulation time of 86.9 seconds.…”

Determination of Phytochemicals, Antimicrobial, Antioxidant and Allelopathic Effects of Fagonia cretica L., collected from Jamshoro, Pakistan

“…Mostly used as an astringent, and febrifuge in different regions of the world and use as a prophylactic drug for various diseases like smallpox, liver trouble, stomach ache, dysentery, fever, typhoid, vomiting, piles, skin diseases, toothache and cancer (Rawal et al, 2004;Hussain et al, 2007;Khan Marwat et al, 2008;Akhtar and Begum, 2009;Ali, 2017;Charan et al, 2021). It worked as a blood purifier and a laxative to release constipation (Chourasia et al, 2014). Its extracts from roots and bark are applied for scabies (Baquar, 1989).…”

Purification, characterization and anticoagulant activity <i>in vitro</i> of polysaccharides from the seeds of <i>Toona sinensi</i>s (A.Juss) Roem

“…Heparins (Wu et al, 2012), are a family of highly suiphated acidic mucopolysaccharides that play an important role as anticoagulants in the treatment of thrombotic disease (Fachina and Verli, 2008;Chourasia et al, 2014). However, they also accompanied with some side effects of causing thrombocytopenia and bleeding (Wu et al, 2012;Ekanayake et al, 2008).…”