Effect of applied and endogenous indol-3-yl-acetic acid on maize root growth

Pilet, Paul‐Emile; Saugy, Martial

doi:10.1007/bf00396089

Cited by 55 publications

(37 citation statements)

References 19 publications

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“…After chilling on ice, the reaction mixture was diluted with 400 pL of water and the IAA methyl-HFB/[13C6]lAA methyl-HFB was extracted twice with 400 pL of hexane. The hexane phase was washed with 400 pL of water and transferred into a small glass tube, dried under a nitrogen stream, and redissolved in 30 pL of hexane (Pilet and Saugy, 1985).…”

Section: Quantification Of Endogenous Hormone Levelsmentioning

confidence: 99%

Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction.

et al. 1995

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We have isolated seven allelic recessive Arabidopsis mutants, designated superroot (surl-7 to surl-7), displaying several abnormalities reminiscent of auxin effects. These characteristics include small and epinastic cotyledons, an elongated hypocotyl in which the connection between the stele and cortical and epidermal cells disintegrates, the development of excess adventitious and lateral roots, a reduced number of leaves, and the absence of an inflorescence. When germinated in the dark, surl mutants did not develop the apical hook characteristic of etiolated seedlings. We were able to phenocopy the Surl-phenotype by supplying auxin to wild-type seedlings, to propagate surl explants on phytohormonedeficient medium, and to regenerate shoots from these explants by the addition of cytokinins alone to the culture medium. Analysis by gas chromatography coupled to mass spectrometry indicated increased levels of both free and conjugated indole-3-acetic acid. surl was crossed to the mutant axr2 and the altered-auxin response mutant ctrl. The phenotype of both double mutants was additive. The surl gene was mapped on chromosome 2 at 0.5 centimorgans from the gene encoding phytochrome B.

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Section: Quantification Of Endogenous Hormone Levelsmentioning

confidence: 99%

Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction.

et al. 1995

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“…The diagnostic ions were at rrdz 278 and 280 corresponding to methyl-ABA and 180-methyl-ABA, monitored in single-ion monitoring (SIM) mode. After methylation, the IAA fractions were derivatized with heptafluorobuturyl imidazole (HFBI) [25]. GC-MS analysis was performed in the electron impact (EI+) mode with the following temperature gradient: 40-95"C (25°C/ min), 95-115°C (5°C/min), 115-300°C (35°C/min).…”

Section: Analysis Of Aba and Laa By Gc-msmentioning

confidence: 99%

Levels of endogenous cytokinins, indole‐3‐acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY‐2 cells

Redig¹,

et al. 1996

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Correlation between cell cycle progression and endogenous levels of plant hormones was studied in synchronized tobacco BY-2 cell suspension cultures. Sixteen different cytokinins, indole-3-acetic acid (IAA) and abscisic acid (ABA) were extracted using solid-phase anion exchange chromatography in combination with immunoaffinity purification, and quantified by mass spectrometry. No significant correlation could be identified for IAA and ABA. In contrast, there were sharp peaks in the levels of specific cytokinins (zeatin-and dihydrozeatin-type) at the end of the S phase and during mitosis. The levels of other cytokinins analyzed, including zeatins N-and O-glucosides, remained low, suggesting that the increased amounts of their corresponding non-glucosylated form resulted from de novo synthesis. These f'mdings suggest that zeatin-and dihydrozeatintype cytokinins might play a specific regulatory role in the progression of the plant cell cycle. One hypothesis to explain cytokinin action is based on a specific interaction with kinases that regulate cell cycle progression, as has been recently shown for the cytokinin analogue olomoucine.

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“…Elucidating mechanisms modulating hormone concentrations is therefore critical to our understanding of certain developmental processes. The phytohormone auxin has been associated with deetiolation (OBrien et al, 1985), root initiation and elongation (Pilet and Saugy, 1985;Norcini and Heuser, 1988;Blakesley et al, 1991a), vascular differentiation (Jacobs, 1976), gravitropism and phototropism (Li et al, 1991), endosperm development (Lur and Setter, 19931, somatic embryogenesis (Michalczuk et al, 1992), and fruit ripening (Slovin and Cohen, 1993). Changes in auxin concentration are often associated with these processes, although the coincident or regulatory nature of auxin concentration is not known.…”

Section: Introductionmentioning

confidence: 99%

A Mutation Altering Auxin Homeostasis and Plant Morphology in Arabidopsis.

et al. 1995

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Many aspects of plant development are associated with changing concentrations of the phytohormone auxin. Severa1 stages of root formation exhibit extreme sensitivities to exogenous auxin and are correlated with shifts in endogenous auxin concentration. In an effort to elucidate mechanisms regulating development of adventitious roots, an ethyl methanesulfonate-mutagenized M2 population of Arabidopsis was screened for mutants altered in this process. A recessive nuclear mutant, rooty (rty), displayed extreme proliferation of roots, inhibition of shoot growth, and other alterations suggesting elevated responses to auxin or ethylene. Wild-type Arabidopsis seedlings grown on auxin-containing media phenocopied rty, whereas rty seedlings were partially rescued on cytokinin-containing media. Analysis by gas chromatography-selected ion monitoring-mass spectrometry showed endogenous indole-3-acetic acid concentrations to be two to 17 times higher in rty than in the wild type. Dose-response assays with exogenous indole-3-acetic acid indicated equal sensitivities to auxin in tissues of the wild type and rty. Combining rty with mutations conferring resistance to auxin (axrl-3) or ethylene (etr7-I) suggested that root proliferation and restricted shoot growth are auxin effects, whereas other phenotypic alterations are due to ethylene. Four mutant alleles fmm independently mutagenized populations were identified, and the locus was mapped using morphological and restriction fragment length polymorphism markers to 3.9 centimorgans dista1 to marker m605 on chromosome 2. The wild-type RTY gene product may serve a critical role in regulating auxin concentrations and thereby facilitating normal plant growth and development.

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Effect of applied and endogenous indol-3-yl-acetic acid on maize root growth

Cited by 55 publications

References 19 publications

Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction.

Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction.

Levels of endogenous cytokinins, indole‐3‐acetic acid and abscisic acid during the cell cycle of synchronized tobacco BY‐2 cells

A Mutation Altering Auxin Homeostasis and Plant Morphology in Arabidopsis.

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