Effect of Antiserum on Maltase from Intestinal Microvillous Membranes of Ascaris suum

Gentner, Harry W.; Castro, Gilbert A.

doi:10.2307/3278687

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…Twenty seven proteins were identified, most individual proteins of which were confined to one or two unique PAGE gel slices ( S2 Table ). Eighteen of the 27 proteins fell into the two major functional categories of peptidases (10) and O-glycosyl hydrolases (eight), of which the glycosyl hydrolases may be related to saccharidase activity previously detected in A. suum intestinal brush border preparations [25] , [26] . Only three proteins (GS_16354, GS_12574, GS_21785) from the entire ConA set were not detected in either of the perfusate fractions, and only one (GS_21785) was absent from both the perfusate and P2 pellet fractions ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…The peptidase activity detected at more alkaline pH notwithstanding, an acidic pH supported peptidase activities from intestinal fractions inclusive of apparent aspartic, serine and metallo peptidases, which is consistent with A. suum proteins identified in intestinal perfusates. Similarly, A. suum intestinal saccharidases associated with AIM brush border preparations (microvilli) had acidic pH preference [25] , [26] . This association with the brush border was suggested to reflect compartmentalization of carbohydrate digestion at the AIM surface that facilitates localized coupling of nutrient transport across the AIM [26] .…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, A. suum intestinal saccharidases associated with AIM brush border preparations (microvilli) had acidic pH preference [25] , [26] . This association with the brush border was suggested to reflect compartmentalization of carbohydrate digestion at the AIM surface that facilitates localized coupling of nutrient transport across the AIM [26] . Our results extend this possibility to include coupling of protein digestion and peptide transport that is facilitated by close association on the AIM.…”

Section: Discussionmentioning

confidence: 99%

“…AIM and IL peptidases have proven valuable as targets in control strategies against parasitic nematodes of humans, animals and plants [26] , [31] . Approaches have included i) vaccination, and ii) delivery of peptide inhibitors and double stranded RNA to disrupt intestinal peptidase functions in multiple parasitic nematodes [31] - [33] .…”

Section: Discussionmentioning

confidence: 99%

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Peptidases Compartmentalized to the Ascaris suum Intestinal Lumen and Apical Intestinal Membrane

2015

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The nematode intestine is a tissue of interest for developing new methods of therapy and control of parasitic nematodes. However, biological details of intestinal cell functions remain obscure, as do the proteins and molecular functions located on the apical intestinal membrane (AIM), and within the intestinal lumen (IL) of nematodes. Accordingly, methods were developed to gain a comprehensive identification of peptidases that function in the intestinal tract of adult female Ascaris suum. Peptidase activity was detected in multiple fractions of the A. suum intestine under pH conditions ranging from 5.0 to 8.0. Peptidase class inhibitors were used to characterize these activities. The fractions included whole lysates, membrane enriched fractions, and physiological- and 4 molar urea-perfusates of the intestinal lumen. Concanavalin A (ConA) was confirmed to bind to the AIM, and intestinal proteins affinity isolated on ConA-beads were compared to proteins from membrane and perfusate fractions by mass spectrometry. Twenty-nine predicted peptidases were identified including aspartic, cysteine, and serine peptidases, and an unexpectedly high number (16) of metallopeptidases. Many of these proteins co-localized to multiple fractions, providing independent support for localization to specific intestinal compartments, including the IL and AIM. This unique perfusion model produced the most comprehensive view of likely digestive peptidases that function in these intestinal compartments of A. suum, or any nematode. This model offers a means to directly determine functions of these proteins in the A. suum intestine and, more generally, deduce the wide array functions that exist in these cellular compartments of the nematode intestine.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Peptidases Compartmentalized to the Ascaris suum Intestinal Lumen and Apical Intestinal Membrane

2015

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“…15,16 The peptidases and glycoside hydrolases are expected to perform basic digestive functions in the intestinal lumen, and proteins from each general category have been recognized as potential vaccine candidates in A. suum and other parasitic nematodes. 27,28 An additional 22 of the 67 proteins were detected in perfusates of the intestinal lumen, many of which likely function in the lumen or in the apical intestinal membrane. Regardless of detection in LC-MS/MS data, it is anticipated that many proteins represented by the FKM cluster 1 have functions that support specialization in the anterior region (e.g.…”

Section: Individual Gene Comparisonsmentioning

confidence: 99%

Compartmentalization of functions and predicted miRNA regulation among contiguous regions of the nematode intestine

et al. 2017

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The intestine of parasitic nematodes has proven an important target for therapies aimed at prevention and treatment of diseases caused by these pathogens in humans, animals and plants. We have developed a unique research model with the intestine of Ascaris suum, the large round worm of swine and humans, that will enhance biological research on this tissue. To expand utility of this model, we quantitatively compared expression of 15,382 coding RNAs and 277 noncoding, micro RNAs (miRNAs) among 3 contiguous regions of the adult A. suum intestine. Differentially expressed transcripts were identified among regions, with the largest number expressed at significantly higher levels in the anterior region, identifying this region as the most functionally unique compared to middle and posterior regions. We further identified 64 exon splice variants (from 47 genes) that are differentially expressed among these regions. A total of 2,063 intestinal mRNA transcripts were predicted to be targeted by intestinal miRNA, and negative correlation coefficients for miRNA:mRNA abundances predicted 22 likely influential miRNAs and 503 likely associated miRNA:mRNA pairs. A. suum intestinal miRNAs were identified that are conserved with intestinal miRNAs from C. elegans (10 mature sequences and 13 seed sequences conserved), and prospective intestinal miRNAs from the murine gastrointestinal nematode, Heligmosomoides polygyrus (5 mature and 11 seeds). Most of the conserved intestinal miRNAs were also high abundance miRNAs. The data provide the most comprehensive compilation of constitutively and differentially expressed genes along the length of the intestine for any nematode species. The information will guide prospective development of many hypotheses on nematode intestinal functions encoded by mRNAs, miRNAs and interactions between these RNA populations.

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Boophilus microplus: Characterization of larval phosphomonoesterases and isolation of subcellular fractions with high phosphatase activity

Zorzopulos¹,

Reich²,

Galassi³

1978

Experimental Parasitology

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Effect of Antiserum on Maltase from Intestinal Microvillous Membranes of Ascaris suum

Cited by 4 publications

References 19 publications

Peptidases Compartmentalized to the Ascaris suum Intestinal Lumen and Apical Intestinal Membrane

Peptidases Compartmentalized to the Ascaris suum Intestinal Lumen and Apical Intestinal Membrane

Compartmentalization of functions and predicted miRNA regulation among contiguous regions of the nematode intestine

Boophilus microplus: Characterization of larval phosphomonoesterases and isolation of subcellular fractions with high phosphatase activity

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