Effect of antiplatelet agents on platelet antistaphylococcal capacity: An in vitro study

Hannachi, Nadji; Grac, Laettitia; Baudoin, Jean-Pierre; Fournier, Pierre‐Edouard; Habib, Gilbert; Camoin-Jau, Laurence

doi:10.1016/j.ijantimicag.2020.105890

Cited by 10 publications

(7 citation statements)

References 19 publications

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“…The washed platelets were used at a concentration of 2.5 × 10 9 /L, adjusting the concentration with PBS. They were incubated with bacteria in a 1:2.5 bacteria-platelet ratio for one hour at 37 °C and identified by expression of the CD41-FITC antibody (4 μL, IgG, Beckman Coulter, Villepinte, France), as previously described [ 31 ]. To determine possible platelet activation following incubation with the bacteria, the expression of the CD62-PC5 antibody (4 μL, IgG, monoclonal, BD sciences, San Jose, CA, USA) on their surface was measured using flow cytometry (Beckman Coulter, FC500, Fullerton, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

The Antibacterial Effect of Platelets on Escherichia coli Strains

et al. 2022

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Platelets play an important role in defense against pathogens; however, the interaction between Escherichia coli and platelets has not been well described and detailed. Our goal was to study the interaction between platelets and selected strains of E. coli in order to evaluate the antibacterial effect of platelets and to assess bacterial effects on platelet activation. Washed platelets and supernatants of pre-activated platelets were incubated with five clinical colistin-resistant and five laboratory colistin-sensitive strains of E. coli in order to study bacterial growth. Platelet activation was measured with flow cytometry by evaluating CD62P expression. To identify the difference in strain behavior toward platelets, a pangenome analysis using Roary and O-antigen serotyping was carried out. Both whole platelets and the supernatant of activated platelets inhibited growth of three laboratory colistin-sensitive strains. In contrast, platelets promoted growth of the other strains. There was a negative correlation between platelet activation and bacterial growth. The Roary results showed no logical clustering to explain the mechanism of platelet resistance. The diversity of the responses might be due to strains of different types of O-antigen. Our results show a bidirectional interaction between platelets and E. coli whose expression is dependent on the bacterial strain involved.

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Section: Methodsmentioning

confidence: 99%

The Antibacterial Effect of Platelets on Escherichia coli Strains

et al. 2022

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“…Indeed, after platelet activation, the bacterium membrane releases molecules into the environment that inhibit bacterial growth. This effect has been mainly demonstrated on S. aureus, but also on other bacterial species, such as Escherichia coli [66][67][68].…”

Section: Involvement Of Platelets In Antibacterial Immunitymentioning

confidence: 99%

The Role of Platelets in Infective Endocarditis

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Hannachi

Gueddari

et al. 2023

IJMS

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Over the last decade, the incidence of infective endocarditis (IE) has increased, with a change in the frequency of causative bacteria. Early evidence has substantially demonstrated the crucial role of bacterial interaction with human platelets, with no clear mechanistic characterization in the pathogenesis of IE. The pathogenesis of endocarditis is so complex and atypical that it is still unclear how and why certain bacterial species will induce the formation of vegetation. In this review, we will analyze the key role of platelets in the physiopathology of endocarditis and in the formation of vegetation, depending on the bacterial species. We provide a comprehensive outline of the involvement of platelets in the host immune response, investigate the latest developments in platelet therapy, and discuss prospective research avenues for solving the mechanistic enigma of bacteria–platelet interaction for preventive and curative medicine.

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“…While studies have shown that platelets have a bactericidal effect against Staphylococcus aureus due to the antimicrobial molecules secreted as a result of platelet activation [ 67 ], this inhibitory effect on Gram-negative bacteria, in particular E. coli , is poorly described. The few available data ( Table 2 ) show that the bactericidal effect on E. coli could depend on many factors, such as the strain tested and the platelet–bacteria ratio [ 54 , 68 ].…”

Section: Mechanism Of Interaction Between Platelets and E Colimentioning

confidence: 99%

Platelets and Escherichia coli: A Complex Interaction

et al. 2022

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Apart from their involvement in hemostasis, platelets have been recognized for their contribution to inflammation and defense against microbial agents. The interaction between platelets and bacteria has been well studied in the model of Staphylococcus and Streptococcus but little described in Gram-negative bacteria, especially Escherichia coli. Being involved in the hemolytic uremic syndrome as well as sepsis, it is important to study the mechanisms of interaction between platelets and E. coli. Results of the published studies are heterogeneous. It appears that some strains interact with platelets through the toll-like receptor-4 (TLR-4) and others through the Fc gamma glycoprotein. E. coli mainly uses lipopolysaccharide (LPS) to activate platelets and cause the release of antibacterial molecules, but this is not the case for all strains. In this review, we describe the different mechanisms developed in previous studies, focusing on this heterogeneity of responses that may depend on several factors; mainly, the strain studied, the structure of the LPS and the platelet form used in the studies. We can hypothesize that the structure of O-antigen and an eventual resistance to antibiotics might explain this difference.

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Effect of antiplatelet agents on platelet antistaphylococcal capacity: An in vitro study

Cited by 10 publications

References 19 publications

The Antibacterial Effect of Platelets on Escherichia coli Strains

The Antibacterial Effect of Platelets on Escherichia coli Strains

The Role of Platelets in Infective Endocarditis

Platelets and Escherichia coli: A Complex Interaction

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