Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study

Salehi, Peyman; Shahrokhi, Seyedeh Zahra; Kamran, T.; Ajami, Ali; Taghiyar, Sana; Deemeh, Mohammad Reza

doi:10.18502/ijrm.v17i2.3987

Cited by 15 publications

(21 citation statements)

References 17 publications

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“…A total of 21 articles published between January 2019 and July 2020 investigated the effects of antioxidant treatment on semen quality ( Table 4 ) [ 25 42 43 44 45 46 56 57 92 93 94 95 96 97 98 99 100 101 104 115 121 ]. Based on our analysis, 13 and 8 studies were ranked as low and high-quality, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…(MedCalc Software bv, Ostend, Belgium). Chi-square test was used to evaluate the association between the quality of the study and the outcomes (positive or no/negative effect) due to antioxidant treatment on semen parameters and sperm function such as oxidative stress and SDF are presented in Table 2 [ 25 26 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 …”

Section: Methodsmentioning

confidence: 99%

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Utility of Antioxidants in the Treatment of Male Infertility: Clinical Guidelines Based on a Systematic Review and Analysis of Evidence

Agarwal

Leisegang

Majzoub

et al. 2021

World J Mens Health

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It is widely accepted that oxidative stress plays an important role in the pathophysiology of male infertility and that antioxidants could have a significant role in the treatment of male infertility. The main objectives of this study are: 1) to systematically review the current evidence for the utility of antioxidants in the treatment of male infertility; and 2) propose evidence-based clinical guidelines for the use of antioxidants in the treatment of male infertility. A systematic review of the available clinical evidence was performed, with articles published on Scopus being manually screened. Data extracted included the type of antioxidant used, the clinical conditions under investigation, the evaluation of semen parameters and reproductive outcomes. The adherence to the Cambridge Quality Checklist, Cochrane Risk of Bias for randomized controlled trials (RCTs), CONSORT guidelines and JADAD score were analyzed for each included study. Further, we provided a Strength Weakness Opportunity Threat (SWOT) analysis to analyze the current and future value of antioxidants in male infertility. Of the 1,978 articles identified, 97 articles were included in the study. Of these, 52 (53.6%) were uncontrolled (open label), 12 (12.4%) unblinded RCTs, and 33 (34.0%) blinded RCTs, whereas 44 (45.4%) articles tested individual antioxidants, 31 (32.0%) a combination of several products in variable dosages, and 22 (22.6%) registered antioxidant products. Based on the published evidence, we 1) critically examined the necessity of additional double-blind, randomized, placebo-controlled trials, and 2) proposed updated evidence-based clinical guidelines for antioxidant therapy in male infertility. The current systematic review on antioxidants and male infertility clearly shows that antioxidant supplementation improves semen parameters. In addition, it provides the indications for antioxidant treatment in specific clinical conditions, including varicocele, unexplained and idiopathic male infertility, as well as in cases of altered semen quality.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Utility of Antioxidants in the Treatment of Male Infertility: Clinical Guidelines Based on a Systematic Review and Analysis of Evidence

Agarwal

Leisegang

Majzoub

et al. 2021

World J Mens Health

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“…While there are no studies to which we can directly compare our results, we can look to the many other studies employing antioxidant-based treatments for comparison. In most studies, employing an antioxidant cocktail (typically containing vitamins C and E, as well as zinc, selenium, l -carnitine and coQ10), or even individual antioxidants such as N -acetyl-cysteine, treatment for 2–3 months significantly reduced sperm DNA fragmentation measured by sperm chromatin structure assay (SCSA), sperm chromatin dispersion (SCD) or TUNEL [ 18 , 19 , 20 , 21 , 22 , 24 , 27 , 52 , 53 ]. In most cases, the average decrease following treatment was modest (<10%) [ 18 , 22 , 24 , 27 , 52 ], despite being statistically significant.…”

Section: Discussionmentioning

confidence: 99%

Mitigating the Effects of Oxidative Sperm DNA Damage

et al. 2020

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Sperm DNA damage is correlated with reduced embryo development and increased miscarriage risk, reducing successful conception. Given its links with oxidative stress, antioxidants have been investigated as a potential treatment, yet results are conflicting. Importantly, individual antioxidants are not identical in composition, and some compounds may be more effective than others. We investigated the use of the polyphenol-rich, high-antioxidant-capacity fruit acai as a treatment for elevated sperm DNA fragmentation (>16%), measured by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL). Following ≥ 74 days of treatment, we observed a significant decrease in sperm DNA fragmentation (−17.0% ± 2.5%) to 11.9 ± 1.7% (0–37%), with a 68.6% success rate (defined as post-treatment TUNEL < 16%). Post-treatment decreases in DNA fragmentation and success rates were not significantly impacted by low motility and/or concentration, or exceptionally high (> 25%) TUNEL. Treatment significantly reduced concentration in men with normal semen parameters, but 88% remained normal. Overall, successful treatment was not associated with age, semen parameters or TUNEL result at baseline. However, body mass index was significantly higher in nonresponders at baseline. This study provides evidence of a low-cost, effective treatment for elevated sperm DNA damage using acai.

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“…That could lead to apoptosis. These antioxidants improve sperm health parameters, and invariably increase fertility (Khaki et al, 2008;Sejian et al, 2014;Salehi et al, 2019). ACSLE increased blood-testis barrier stability (Jedlinska-Krakowska et al, 2006) and improved sperm quality of breeder stocks that were fed with vitamin C supplemented feeds (Ezzat et al, 2011;Okoro et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Onion contains exogenous and endogenous antioxidants like selenium, glutathione, vitamins A, B, and C and flavonoids such as quercetin and isorhamnetin (Griffiths et al, 2002). These antioxidants protect DNA and other important molecules from peroxidation damage that can lead to apoptosis, and invariably improve sperm health parameters, and hence increase fertility (Khaki et al, 2008;Sejian et al, 2014;Salehi et al, 2019). The biological action of Allium products is ascribed to its organo-sulphur and phenolic compounds (Kumud et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Effects of <i>Allium cepa</i> L. peels extract on gonadotropins, testosterone and sperm variables in Oba Marshal broiler cocks

Adeleye¹,

Oguntayo²,

Adeleye³

et al. 2020

Sokoto J. Vet. Sc.

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Allium cepa (onion), a natural seasoning agent that contains significant amounts of potent antioxidants in its scaly leaves is used in folkloric medicine to manage several diseases globally. Antioxidants have an essential effect on sperm health parameters; however, there is limited information on the effects of Allium cepa scaly leaf extract on reproductive functions in Oba Marshal breeder cocks. This study was conducted to investigate the effects of the aqueous extract of Allium cepa scaly leaf on reproductive functions in sexually matured Oba Marshal breeder cocks. Allium cepa bulbs were obtained from a market in Abeokuta, Ogun State. Dry scaly leaves were peeled, pulverised, macerated in distilled water, filtered and concentrated. Twenty, 42 weeks old Oba Marshal breeder cocks (3.48 – 3.62 kg) were divided into 4 groups (n = 5) and treated daily for 2 weeks thus: CT (control, distilled water, 0.5 mL/kg), T2 (extract 200 mg/kg/bird), T4 (extract 400 mg/kg/bird), T8 (extract 800 mg/kg/bird). Sperm characteristics were assessed microscopically. Testosterone, follicle stimulating hormone (FSH) and luteinizing hormone (LH) were analysed using ELISA. Data were analysed using ANOVA at α0.05. Treated birds had significantly (p < 0.05) higher sperm motility, morphology but non-significant changes in sperm viability and concentration compared with the controls. Also, serum FSH and LH significantly increased, while testosterone had no significant change in test groups compared to the control. Aqueous extract of Allium cepa scaly leaf improved testicular functions and morphology in the test cocks. The reproductive function enhancement of the extract may be due to its antioxidant effect. Keywords: Allium cepa, Breeding, Broiler cocks, Spermatogenesis, Testosterone

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Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study

Cited by 15 publications

References 17 publications

Utility of Antioxidants in the Treatment of Male Infertility: Clinical Guidelines Based on a Systematic Review and Analysis of Evidence

Utility of Antioxidants in the Treatment of Male Infertility: Clinical Guidelines Based on a Systematic Review and Analysis of Evidence

Mitigating the Effects of Oxidative Sperm DNA Damage

Effects of <i>Allium cepa</i> L. peels extract on gonadotropins, testosterone and sperm variables in Oba Marshal broiler cocks

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