Effect of antioxidant on in-vitro maturation of vitrified bovine oocytes

Abstract

The present study was undertaken to investigate the effect of -tocopherol on in-vitro maturation of vitrified bovine oocytes. The cumulus-oocyte-complexes (COCs) recovered from follicles (3–5 mm diameter) by aspiration and slicing techniques were equally divided into 5 (five) groups consisting 66 COCs in each. The vitrified bovine oocytes were sub-grouped into control and treatment group. The vitrified- and non-vitrified- control group of oocytes were in-vitro matured in TCM-199 media without supplementation of vitamin E. In three vitrified treatment groups, the oocytes were in-vitro cultured in TCM-199 media supplemented with vitamin E (-tocopherol) @ 50 μM, 100 μM and 200 μM, respectively. The mean percentage of cumulus cell expansion and polar body formation in non-vitrified control and vitrified control groups were 85.18±2.57 and 65.38±1.83, and 51.67±1.94 and 30.26±0.16, respectively. The rate of cumulus cell expansion and polar body formation in the oocytes of three vitrified treatment groups media supplemented with vitamin E @ 50 μM, 100 μM and 200 μM were 56.39±3.49 and 34.62±1.83, 69.95±3.20 and 56.23±1.61, and 54.44±4.73 and 31.62±4.50% respectively. Mean percentage of both cumulus cell expansion and polar body formation in the non-vitrified control group were significantly higher than that in the vitrified control group. In the treatment group supplemented with vitamin E @ 100 μM, both cumulus cells expansion and polar body formation were significantly higher than that in media supplemented with 50 μM and 200 μM vitamin E as well as in vitrified control group.

Effects of Ascorbic Acid on Maturation Rate, Morphology, and Gene Expression of Vitrified In Vitro Matured Dromedary Camel Oocytes

“…The results of the current investigation showed that AA significantly improved the recovery rate of vitrified/thawed mature camel oocytes and reduced the appearance of abnormal oocyte morphology in comparison to the control. These findings are in agreement with Sonowal et al (2017) who reported that the addition of a specific dose (100 M) of Vitamin C to in vitro maturation medium when used with immature bovine oocytes, increased the growth of vitrified-thawed bovine oocytes in terms of cumulus cell expansion and polar body formation after maturation. However, Chaves et al (2017) found that immature COCs were more resistant to the effects of cryoprotectants than mature COCs and this result was confirmed by superior embryo growth (cleavage) after vitrification.…”

Meta-Analysis of the Prevalence of Porcine Zoonotic Bacterial Pathogens in India: A 13-Year (2010–2023) Study

“…[64,65,67]. In the present study, it was also observed that the prevalence of Streptococcus suis was on par with the findings of several researchers [90,94,96].…”

Astaxanthin Added during Post-Warm Recovery Mitigated Oxidative Stress in Bovine Vitrified Oocytes and Improved Quality of Resulting Blastocysts

“…However, the endogenous antioxidant system is insufficient in vitro; therefore, adding exogenous antioxidants could be an option to improve in vitro culture conditions [10]. Several authors in their studies [11][12][13][14] used antioxidants in maturation, vitrification, or culture media to increase cryotolerance and viability of oocytes after vitrification and subsequent embryo development. In recent years, there has been a significant interest in testing bioactive molecules of marine origin, as they showed high potential for health benefits.…”