Effect of antimicrobial peptides and monoterpenes on control of fire blight

Abstract

Aim of study: Antimicrobial peptides and monoterpenes are safe compounds that have been used for control of many plant diseases. Herein, the effects of two recombinant antibacterial peptides (AMPs) were compared with two monoterpenes for control of Erwinia amylovora directly or via induction of plant defense enzyme guaiacol peroxidase (GPOD).Area of study: The experiments were performed at the Ferdowsi University of Mashhad (Iran).Material and methods: The central composite design (CCD) method was used to study the effect of mixing the compounds and copper compound (Nordox) in controlling the pathogen. The resistance level was studied on shoots of tolerant (‘Dargazi’) and semi-susceptible (‘Spadona’) pear cultivars treated with the antibacterial compounds.Main results: Thanatin and 1,8-cineole showed the highest and lowest antibacterial effects. All treatments reduced E. amylovora pathogenicity on blossom. The CCD analysis revealed that the best reduction in colony number obtained by mixing Lfc, thanatin, thymol, 1,8-cineole and Nordox at concentrations of 32, 16, 24, 250 and 250 μg/mL. Thymol and 1,8-cineole at 500 μg/mL decreased disease severity significantly compared to that of AMPs. The level of GPOD enzyme in ‘Dargazi’ was higher than in ‘Spadona’. All treatments increased the GPOD levels in both cultivars. Furthermore, resistance level and GPOD ratio were negatively correlated.Research highlights: Antimicrobial peptides showed better effect on growth inhibition of E. amylovora than monoterpenes. Mixing of these peptides and monoterpens at special dosage enhanced their antimicrobial efficacy against E. amylovora; that could represent a new method in control of fire blight disease.

Evaluation of Three Antimicrobial Peptides Mixtures to Control the Phytopathogen Responsible for Fire Blight Disease

“…The effects of AMPs on E. amylovora and their use as a control measure for this bacterium have been reported in several studies over the last 15 years [16,[27][28][29][30][31]. Recently, we have demonstrated the bioactivity of two AMPs against E. amylovora [17], namely, RW-BP100 and CA-M, which had already been reported to display activity against pathogenic bacteria but never tested against the causative agent of the fire blight disease [32][33][34].…”

Recent Advances and Multiple Strategies of Monoterpenoid Overproduction in Saccharomyces cerevisiae and Yarrowia lipolytica

“…Their versatility and effectiveness in various applications, from industrial to consumer products, underscore their important advantage in their development and utilization. 4,5 Nowadays, plant extraction or chemical synthesis are the main ways to acquire monoterpenoids. Monoterpenoids are commonly found in the secretory tissues of higher plants and can be extracted using processes like steam decomposition.…”

“…Indeed, as high-potential feedstocks, monoterpenoid compounds hold a promising future due to their substantial value in numerous essential fields. Their versatility and effectiveness in various applications, from industrial to consumer products, underscore their important advantage in their development and utilization. , …”