Effect of Antimicrobial Denture Base Resin on Multi-Species Biofilm Formation

Abstract

Our aims of the research were to study the antimicrobial effect of dimethylaminododecyl methacrylate (DMADDM) modified denture base resin on multi-species biofilms and the biocompatibility of this modified dental material. Candida albicans (C. albicans), Streptococcus mutans (S. mutans), Streptococcus sanguinis (S. sanguinis), as well as Actinomyces naeslundii (A. naeslundii) were used for biofilm formation on denture base resin. Colony forming unit (CFU) counts, microbial viability staining, and 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) array were used to evaluate the antimicrobial effect of DMADDM. C. albicans staining and Real-time PCR were used to analyze the morphology and expression of virulence genes of C. albicans in biofilm. Lactate dehydrogenase (LDH) array and Real-time PCR were conducted to examine the results after biofilm co-cultured with epithelial cell. Hematoxylin and eosin (HE) staining followed by histological evaluation were used to study the biocompatibility of this modified material. We found that DMADDM containing groups reduced both biomass and metabolic activity of the biofilm significantly. DMADDM can also inhibit the virulence of C. albicans by means of inhibiting the hyphal development and downregulation of two virulence related genes. DMADDM significantly reduced the cell damage caused by multi-species biofilm according to the LDH activity and reduced the expression of IL-18 gene of the cells simultaneously. The in vivo histological evaluation proved that the addition of DMADDM less than 6.6% in denture material did not increase the inflammatory response (p > 0.05). Therefore, we proposed that the novel denture base resin containing DMADDM may be considered as a new promising therapeutic system against problems caused by microbes on denture base such as denture stomatitis.

Synthesis, Antifungal Activity, and Biocompatibility of Novel 1,4-Diazabicyclo[2.2.2]Octane (DABCO) Compounds and DABCO-Containing Denture Base Resins

“…Several other experimental antimicrobial denture materials have been developed, including a denture base resin containing dimethylaminododecyl methacrylate (DMADDM) (23). DMADDM is a quaternary ammonium salt (QAS) that can be copolymerized with acrylic denture base resin.…”

Resumptive Streptococcus mutans Persisters Induced From Dimethylaminododecyl Methacrylate Elevated the Cariogenic Virulence by Up-Regulating the Quorum-Sensing and VicRK Pathway Genes

“…Quaternary ammonium salts (QASs) can incorporate into many dental materials to combat oral bacterial species (Imazato, 2009;Cheng et al, 2017) by damaging the cell membrane (Beyth et al, 2006). A novel quaternary ammonium monomer, dimethylaminododecyl methacrylate (DMADDM) was proved to be a potential anti-caries dental material due to its long-lasting and remarkable antibacterial effects and well biocompatibility in vitro and in vivo (Cheng et al, 2013;Mei et al, 2013;Wang et al, 2016;Zhang et al, 2016). We identified that lethal concentration of DMADDM can induce the formation of S. mutans persisters by decreasing the metabolism for the first time (Jiang et al, 2017).…”

Application of cationic polymer micelles for the dispersal of bacterial biofilms

“…The effort in future studies should be directed to the elaboration of appropriate models of multi-species biofilms. As recent advances in this direction, model communities of multi-species biofilms relevant to dental medicine should be mentioned [35,36]. We expect that further work in this direction will provide novel laboratory models useful for the tests of anti-fouling substances.…”