Effect of antihyperlipidemic polyherbal formulation in high diet induced hyperlipidemia wistar albino rats. An in vitro - in vivo evaluation

Abstract

Hyperlipidemia is a prevalent global health problem, and it is linked to various cardiovascular disorders. The side effects of the current lipid-lowering drugs have increased the tendency to move toward traditional and alternative remedies. The study aims to Formulate and evaluate the Antilipidemic activity of Polyherbal formulations used as a traditional medicine in the Malabar area, Kerala by in vitro and in vivo methods. Further, the present study also compares the impact of seasonal variations on chemical contents of ingredient herbs of polyherbal medicine. They were analyzed for, anti-oxidant activity by DPPH and Nitric oxide method and In-vitro anti-cholesterol activity by cholesterol enzymatic endpoint method using simavastatin as a positive control. The formulation showing highest anti-oxidant and in- vitro Antilipidemic activity was selected for in-vivo analysis. Out of four formulations, PHF 1 shows low IC50 values in DPPH and Nitric oxide methods (250.45± 0.60, 985.40±5.59), respectively. The in-vitro anti-cholesterol activity showed a maximum % of inhibition for PHF1. Based on this PHF 1was selected for in vivo analysis. Acute toxicity was performed according to OECD guidelines. The antilipidemic activity was conducted by Diet-induced hyperlipidemia model in Wistar albino rats, containing six animals in each group. All the groups except saline control received a high-fat diet for two weeks. The Polyherbal formulation (200 mg/kg & 400 mg/kg) showed significant (P<0.05) reduction in total serum cholesterol and lipid levels compared to the vehicle control group. This present study proved that Polyherbal formulation has Antilipidemic activity against the diet-induced hyperlipidemia model by reducing the total serum cholesterol (TC), triglycerides (TG), very low-density lipid (VLDL), low-density lipids(LDL) levels and increasing high-density lipid (HDL) level.