Effect of anticonvulsants on seizures developing in the course of daily administration of pentetrazol to rats

Ito, Tsugutaka; Hori, Misako; Yoshida, Kouichi; Shimizu, Masanao

doi:10.1016/0014-2999(77)90086-3

Cited by 82 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the anticonvulsant action of drugs in PTZ kindling model differs from that described in the s.c. PTZ seizure model [15]. Taken together, the anticonvulsant effect of lacosamide described in this study is not in contradiction with previously reported results.…”

Section: Discussionsupporting

confidence: 70%

Lacosamide Reduces Seizure Severity but Increases Seizure Frequency in PTZ-Kindled Rats

Gáll

Koncz

Gáll

et al. 2017

Acta Medica Marisiensis

View full text Add to dashboard Cite

Objective: This study evaluated the anticonvulsant action of lacosamide (LCS), a novel drug that was recently approved for the treatment of partial or secondarily generalized seizures, using an animal model of generalized epilepsy induced by repetitive pentylenetetrazole (PTZ) administration in rats. The main goal was to evaluate the behavioral pattern of lacosamide action by classifying seizures according to a modified Racine-scale. Furthermore, the reproducibility of the win-PTZ kindling model of epilepsy, a recently described variant of the standard PTZ-kindling model, was also assessed. Methods: Adult male Wistar rats (n=16) were divided into two groups and underwent the win-PTZkindling protocol in two independent trials. After finishing the kindling procedure, all animals, which presented stage 5 seizures were tested for the anticonvulsant action of lacosamide at three different doses (3, 10, and 30 mg/kg). Results: The maximal severity of seizures decreased and the latency to stage 3-5 seizures increased when the animals were treated with lacosamide at a single dose of 10 mg/kg compared to saline pretreatment (p < 0.05), both parameter reflecting an anticonvulsant action of the drug. Unfortunately, the number of stage 3-5 seizures also increased, but not significantly. The win-PTZ kindling model showed an adequate reproducibility between different trials, however, the number of fully kindled rats was lower than previously reported. Conclusions: Lacosamide showed a convincing anticonvulsant action in the win-PTZ kindling model of epilepsy by preventing the generalization of seizures. The win-PTZ kindling model was proved to be useful for studying epileptogenesis and the anticonvulsant action of drugs.

show abstract

Section: Discussionsupporting

confidence: 70%

Lacosamide Reduces Seizure Severity but Increases Seizure Frequency in PTZ-Kindled Rats

Gáll

Koncz

Gáll

et al. 2017

Acta Medica Marisiensis

View full text Add to dashboard Cite

show abstract

“…To the best of our knowledge, no study is available to demonstrate the per se effect of etoricoxib on seizures. In this study, since the kindling model is believed to correlate with epileptogenesis and kindling induces long-lasting changes such that seizure sensitivity to subconvulsant dose persists for 4 to 10 months [14], the effects of chronic treatment with the selective COX-2 inhibitor etoricoxib in pentylenetetrazole-kindled seizures and behavioral effects in rats were determined.…”

Section: Introductionmentioning

confidence: 99%

Anticonvulsant activity of the cyclooxygenase-2 (COX-2) inhibitor etoricoxib in pentylenetetrazole-kindled rats is associated with memory impairment

Katyal

Kumar

Gupta

2015

Epilepsy & Behavior

View full text Add to dashboard Cite

“…,26 Thus, the overall goal of this study was to explore the contribution of Sx c − to epileptogenesis by comparing PTZ kindling in mice wild-type or null for the SLC7A11 gene.…”

mentioning

confidence: 99%

Decreased epileptogenesis in mice lacking the System x_c⁻ transporter occurs in association with a reduction in AMPA receptor subunit GluA1

Sears

Hewett

2019

Epilepsia Open

View full text Add to dashboard Cite

Summary Objective Although the cystine/glutamate antiporter System xc− (Sxc−) plays a permissive role in glioma‐associated seizures, its contribution to other acquired epilepsies has not been determined. As such, the present study investigates whether and how Sxc− contributes to the pentylenetetrazole (PTZ) chemical kindling model of epileptogenesis. Methods Male Sxc− null (sut/sut) mice and their wild‐type littermates were administered PTZ (i.p.) daily for up to 21 days (kindling paradigm). Seizure severity was scored on a 5‐point behavioral scale. Mossy fiber sprouting, cellular degeneration, and Sxc− light chain (xCT) messenger RNA (mRNA) were explored using Timm staining, thionin staining, and real‐time quantitative polymerase chain reaction (qPCR), respectively. Levels of reduced and oxidized glutathione and cysteine were determined via high‐performance liquid chromatography (HPLC). Plasma membrane protein levels of glutamate and γ‐aminobutyric acid (GABA) receptor subunits as well as the K+/Cl− co‐transporter KCC2 were quantified via western blot analysis. Results Repeated administration of PTZ produced chemical kindling in only 50% of Sxc− null mice as compared to 82% of wild‐type littermate control mice. Kindling did not result in any changes in xCT mRNA levels assessed in wild‐type mice. No cellular degeneration or mossy fiber sprouting was discernible in either genotype. Except for a small, but significant, decrease in oxidized cysteine in the hippocampus, no other change in measured redox couples was determined in Sxc− null mice. Cortical levels of the α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) receptor subunit GluA1 were decreased in Sxc− null mice as compared to wild‐type littermates, whereas all other proteins tested showed no difference between genotypes. Significance This study provides the first evidence that Sxc− signaling contributes to epileptogenesis in the PTZ kindling model of acquired epilepsy. Further data indicate that a reduction in AMPA receptor signaling could underlie the resistance to PTZ kindling uncovered in Sxc− null mice.

show abstract

Effect of anticonvulsants on seizures developing in the course of daily administration of pentetrazol to rats

Cited by 82 publications

References 14 publications

Lacosamide Reduces Seizure Severity but Increases Seizure Frequency in PTZ-Kindled Rats

Lacosamide Reduces Seizure Severity but Increases Seizure Frequency in PTZ-Kindled Rats

Anticonvulsant activity of the cyclooxygenase-2 (COX-2) inhibitor etoricoxib in pentylenetetrazole-kindled rats is associated with memory impairment

Decreased epileptogenesis in mice lacking the System x_c⁻ transporter occurs in association with a reduction in AMPA receptor subunit GluA1

Contact Info

Product

Resources

About

Effect of anticonvulsants on seizures developing in the course of daily administration of pentetrazol to rats

Cited by 82 publications

References 14 publications

Lacosamide Reduces Seizure Severity but Increases Seizure Frequency in PTZ-Kindled Rats

Lacosamide Reduces Seizure Severity but Increases Seizure Frequency in PTZ-Kindled Rats

Anticonvulsant activity of the cyclooxygenase-2 (COX-2) inhibitor etoricoxib in pentylenetetrazole-kindled rats is associated with memory impairment

Decreased epileptogenesis in mice lacking the System xc− transporter occurs in association with a reduction in AMPA receptor subunit GluA1

Contact Info

Product

Resources

About

Decreased epileptogenesis in mice lacking the System x_c⁻ transporter occurs in association with a reduction in AMPA receptor subunit GluA1