Effect of antibiotics on motion characteristics of cooled stallion spermatozoa

Jasko, D.J.; Bedford, Sylvia J.; Cook, Nigel; Mumford, Elizabeth; Squires, E.L.; Pickett, B.W.

doi:10.1016/0093-691x(93)90356-a

Cited by 39 publications

(31 citation statements)

References 9 publications

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“…Tested extenders contained different antibiotics, Polimixin B in EZ and EZ‐Y, gentamicin sulphate and Na‐penicillin in INRA82‐Y. Polimixin and gentamicin may negatively influence sperm motility (Jasko et al. 1993).…”

Section: Discussionmentioning

confidence: 99%

Studies on Motility and Fertility of Cooled Stallion Spermatozoa

Rota

Furzi

Panzani

et al. 2004

Reprod Domestic Animals

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This study on extended, cooled stallion spermatozoa aimed to compare the ability of three extenders to maintain sperm motility during 24 h of preservation, and to describe pregnancy and foaling rates after artificial insemination (AI) of stallion spermatozoa stored and transported in the extender chosen from the in vitro study. After 6 and 24 h of preservation, motility, both subjective and evaluated by the motility analyzer (total, progressive and rapid), was lower in non-fat, dried skim milk-glucose than in both other extenders: dried skim milk-glucose added to 2% centrifuged egg yolk, and ultra high temperature treated skim milk-sugar-saline solution added to 2% centrifuged egg yolk (INRA82-Y). Rapid spermatozoa and sperm velocity parameters, after 24 h, were significantly higher in INRA82-Y. In the fertility trial, semen collected from three Maremmano stallions, diluted in INRA82-Y, and transported in a refrigerated Styrofoam box, was used to inseminate 56 mares of the same breed. Pregnancy rates after the first cycle and per breeding season were significantly higher for the 31 mares inseminated in three AI centres (54.8 and 80.6%, respectively) than for the 25 mares inseminated at the breeder's facilities (28.0 and 52.0%). Foaling rates were not significantly different between the AI centres mares (54.8%) and the other mares (44.0%). In conclusion, INRA82-Y yielded satisfactory pregnancy and foaling rates, especially when employed in the more controlled situation of an AI centre, and can therefore be included among those available for cooled stallion semen preservation.

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Section: Discussionmentioning

confidence: 99%

Studies on Motility and Fertility of Cooled Stallion Spermatozoa

Rota

Furzi

Panzani

et al. 2004

Reprod Domestic Animals

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“…1998). Traditionally, antibiotics have been added to semen extenders to inhibit bacterial growth in stored semen and protect the mare from post‐coital endometritis (Jasko et al. 1993).…”

Section: Introductionmentioning

confidence: 99%

Effects of Oxygen Exposure and Gentamicin on Stallion Semen Stored at 5 and 15°C

Price

Aurich

Davies-Morel³

et al. 2007

Reprod Domestic Animals

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This study was undertaken to investigate the effects of storage of stallion semen in a defined milk protein extender at 5 and 15 degrees C under either anaerobic or aerobic conditions, with or without addition of the antibiotic gentamicin. Semen samples were collected from eight fertile stallions and stored for 96 h (day 0-4) and assessed daily for motility, velocity and membrane integrity (viability) using a CASA system. Samples for bacteriology assessment were taken on day 2 of storage. No significant (p > 0.05) differences in motility, velocity or viability were observed between treatments on days 0-2. On days 3 and 4, semen stored without gentamicin at 5 degrees C had a significantly (p < 0.05) better semen quality compared with storage at 15 degrees C without gentamicin, irrespective of oxygen exposure. On days 3 and 4, motility and velocity were greater in samples stored at 15 degrees C with gentamicin, compared with the corresponding treatments without antibiotic (p < 0.05). This effect was also evident for viability on day 4. The decline in semen quality observed at 15 degrees C most likely resulted from the effect of bacterial growth. Bacterial growth was the greatest in samples stored at 15 degrees C without gentamicin, under both anaerobic and aerobic conditions (p < 0.05). Bacterial growth was inhibited by adding of gentamicin at 15 degrees C, which accordingly reduced the decline in semen quality. Addition of antibiotic to samples stored at 5 degrees C had no significant effect on any parameter analysed. In conclusion, storage at 15 degrees C can be achieved by using an extender containing the antibiotic gentamicin. Storage at 5 degrees C tended to maintain better semen quality irrespective of oxygen exposure, and did not necessitate an antibiotic treatment.

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“…Varner et al (1998) reported gentamicin sulfate to be more effective in controlling bacterial growth i n stallion semen t h a n Polyinixiii B, sodium penicillin or ticarcillin. Jasko et al (1993 ) have reported gentamicin to be toxic to spermatozoa but Squires et al (1981) and Varner et al (1998) found no such effect on motility or velocity of sperm. The present study was designed to determine the effects of various concentrations of gentamicin sulfate in semen extender on motility and velocity.…”

Section: Introductionmentioning

confidence: 99%