Effect of antibiotics on cell proliferation in the Closterium peracerosum-strigosum-littorale complex (Charophyceae, Chlorophyta)

Abstract

Abstract:The effects of several antibiotics on the proliferation of cells of the Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga, were examined. When cells were cultured on solid medium containing hygromycin B and phleomycin the proliferation of cells was inhibited at low concentrations of these antibiotics, with a minimum inhibitory concentration of 5.0 and 0.2 µg/mL, respectively. By contrast, kanamycin sulfate was less effective at concentrations up to 50 µg/mL. When cells were incubated in liquid medium containing hygromycin B and phleomycin, cell proliferation was severely inhibited at concentrations of 5.0 and 0.01 µg/mL, respectively. It is concluded that hygromycin B and phleomycin are highly effective for inhibiting the proliferation of C. psl. complex both on solid and in liquid medium and thus are useful for the selection of the cells transformed by selectable marker genes.

Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum‐strigosum‐littorale complex by square‐pulse electroporation

“…To obtain stable transformants, we incubated the electroporated cells in the presence of hygromycin (10 µg ml –1 ). We previously demonstrated that wild‐type C. psl complex cells cannot proliferate in hygromycin (5 μg ml –1 ) (Abe et al ., 2008b). Here, no surviving cells were observed when 1 × 10 4 nonelectroporated control cells were incubated in 5 or 10 μg ml –1 hygromycin for 14 d. After 3 wk of selection, a number of surviving cells were obtained.…”

Sexual Reproduction of a Unicellular Charophycean Alga, Closterium peracerosum-strogosum-littorale Complex

“…Phleomycin is a useful antibiotic for the selection of stable transformants in the C. psl. complex because the drug inhibits cell proliferation at low concentrations both in liquid media and on solid media (Abe et al 2008b ). The overexpression vector pSA1102, which allowed direct selection by phleomycin and the overexpression of the arbitrary genes, was constructed (Fig.…”