Effect of Anti-oxidant, Anti-inflammatory and Anti-invasive of PMA-induced Matrix Metalloproteinase (MMP-2) and MMP-9 Activities of Water Extract and Solvent Fractions of Saururus Chinensis

Abstract

Saururus chinensis is a perennial plants, its flavonoid compound is known to exhibit anti-oxidative activity. This study was aimed to investigate the effect of Water Extract and Solvent Fractions of Saururus chinensis on antioxidant, anti-inflammatory and anti-invasive of Phorbol 12-myristate 13-acetate (PMA)-induced matrix metalloproteinase (MMP-2) and MMP-9 activities. Plant samples were fractionated into hexane, CHCl3, ethyl acetate, butanol, and water fractions, and each of these was assayed individually. The water fraction showed the highest extraction yield at 9.25%(w/w). Anti-oxidative activity was analyzed by DPPH assay. Cell viability was detected by the MTS assay. Anti-inflammatory activity was assayed by the nitric oxide (NO) production in mouse macrophage Raw 264.7 cells. The activity and mRNA expression of MMP-2 and MMP-9 in human oral squamous carcinoma YD-10B cells were examined by zymography and RT-PCR. As results, MMP-2/-9 activation was increased in PMA induced YD-10B cells. In PMA-treated YD-10B cells, the increased mRNA expression and protein activation of MMP-2/-9 were significantly inhibited in the ethyl acetate fraction. The ethyl acetate fraction showed the highest anti-oxidative activity at 73.38%. The ethyl acetate fraction at non-cytotoxic concentrations significantly exhibited the anti-inflammatory activity of Raw 264.7 cells in dose-dependent manner. In conclusion, these findings demonstrate that the ethyl acetate fraction obtained from a chinensis water extract potentiates a promising therapeutic anti-invasive agent and, therefore, as an anti-cancer drug for cancer prevention and therapy in oral cancer.Key words : Anti-oxidant, anti-inflammatory, anti-invasive, matrix metalloproteinases, Saururus chinensis *Corresponding author *Tel : +82-33-738-7683, Fax : +82-33-738-7683 *E-mail : jung0724@sangji.ac.kr This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Effect of the Solvent Fractions of Zingiber officinale Roscoe against Thrombintreated Tumor Invasion in Human Oral Squamous Carcinoma YD-10B Cells

“…구강암은 전체 암의 약 3~5% 정도로, 이 중 편평세포암종 (Squamous cell carcinoma, SCC)은 약 90%를 차지하고 있다 [10,13,14]. 구강편평세포암종의 발생은 환경, 유전적 배경, 호르몬 등의 다양한 원인 인자들의 복잡한 상호 연관성과 함 께 유전적인 손상이 지속적으로 진행되어 다단계의 발생과정 을 거친다.…”

“…구강편평세포암종의 발생은 환경, 유전적 배경, 호르몬 등의 다양한 원인 인자들의 복잡한 상호 연관성과 함 께 유전적인 손상이 지속적으로 진행되어 다단계의 발생과정 을 거친다. 또한 다른 암과는 달리 구강암은 조기 발견과 치료 가 용이하여 암 예방을 위한 좋은 모델로 인정받고 있다 [13,14,26].…”

Effects of Saururus chinensis BAILL Extract in Rats with Experimentally Chronic Constipation: An application of Clinical Pathology and Digital Image Processing

“…현재 사용되고 있는 변비 치료 또는 개선 약물은 폐쇄 형변비의 경우 60~80%라는 높은 치료효과를 나타내고 있지만 서행성변비 혹은 장무력증에 의한 변비의 치료율 이 저조하여 효과가 검증되지 않은 민간요법으로 변비를 해결하려는 사람들이 증가하고 있는 추세이다 (Cheon et al, 2007 (Lee et al, 2000;Lee et al, 2006), 전통적으로 요도염, 전립선염, 방광염, 노동피로, 근육통, 골수염에 효 과가 있다고 있다고 알려져 있다 (Lee et al, 2002). 또한 삼 백초 추출물은 MMP-2와 MMP-9의 활성을 억제하여 항 염증효과 및 항산화효과가 있다는 연구결과가 보고되고 있다 (Kim and Kim, 2016 (Shi et al, 2010;Singh et al, 2012). P<0.01 (compare with Group I); ## P<0.01 (compare with Group II).…”