Effect of angiotensin-(1-7) on reperfusion arrhythmias in isolated rat hearts

Neves, Liomar A. A.; Almeida, Alvair P.; Khosla, M. C.; Campagnole‐Santos, Maria José; Santos, Robson Augusto Souza

doi:10.1590/s0100-879x1997000600016

Cited by 60 publications

(48 citation statements)

References 33 publications

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“…Release of vasoconstrictor prostaglandins by Ang-(1-7) in the rat coronary circulation has been suggested, although higher concentrations of this peptide were used (17).…”

Section: Discussionmentioning

confidence: 99%

“…We have also demonstrated in hearts perfused with Krebs-Ringer solution (KRS) that Ang-(1-7) produced a concentration-dependent (27-210 nM) reduction in coronary flow (25% reduction at highest concentration), while only slight and variable changes in contraction force and heart rate were observed. Under the same conditions, Ang II (27 and 70 nM) produced a significant reduction in coronary flow (39 and 48%, respectively) associated with a significant increase in force (17). In contrast, Almeida et al (18) have shown that in isolated rat hearts Ang-(1-7) induced an increase in the vasodilator effect of bradykinin (BK) through a nitric oxide (NO) and prostaglandin release-related mechanism.…”

Section: Introductionmentioning

confidence: 97%

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Angiotensin-(1-7) improves the post-ischemic function in isolated perfused rat hearts

Ferreira

Santos

Almeida

2002

Braz J Med Biol Res

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We evaluated the effects of angiotensin-(1-7) (Ang-(1-7)) on postischemic function in isolated hearts from adult male Wistar rats perfused according to the Langendorff technique. Local ischemia was induced by coronary ligation for 15 min. After ischemia, hearts were reperfused for 30 min. Addition of angiotensin II (Ang II) (0.20 nM, N = 10) or Ang-(1-7) (0.22 nM, N = 10) to the Krebs-Ringer perfusion solution (KRS) before the occlusion did not modify diastolic or systolic tension, heart rate or coronary flow (basal values for Ang-(1-7)-treated hearts: 0.72 ± 0.08 g, 10.50 ± 0.66 g, 216 ± 9 bpm, 5.78 ± 0.60 ml/min, respectively). During the period of occlusion, the coronary flow, heart rate and systolic tension decreased (values for Ang-(1-7)-treated hearts: 2.83 ± 0.24 ml/min, 186 ± 7 bpm, 6.95 ± 0.45 g, respectively). During reperfusion a further decrease in systolic tension was observed in control (4.95 ± 0.60 g) and Ang II-treated hearts (4.35 ± 0.62 g). However, in isolated hearts perfused with KRS containing Ang-(1-7) the further reduction of systolic tension during the reperfusion period was prevented (7.37 ± 0.68 g). The effect of Ang-(1-7) on the systolic tension was blocked by the selective Ang-(1-7) antagonist A-779 (2 nM, N = 9), by the bradykinin B 2 antagonist HOE 140 (100 nM, N = 10), and by indomethacin pretreatment (5 mg/ kg, ip, N = 8). Pretreatment with L-NAME (30 mg/kg, ip, N = 8) did not change the effect of Ang-(1-7) on systolic tension (6.85 ± 0.61 g). These results show that Ang-(1-7) at low concentration (0.22 nM) improves myocardial function (systolic tension) in ischemia/reperfusion through a receptor-mediated mechanism involving release of bradykinin and prostaglandins.

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“…Release of vasoconstrictor prostaglandins by Ang-(1-7) in the rat coronary circulation has been suggested, although higher concentrations of this peptide were used (17).…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Angiotensin-(1-7) improves the post-ischemic function in isolated perfused rat hearts

Ferreira

Santos

Almeida

2002

Braz J Med Biol Res

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“…Kumagai et al observed similar results in isolated hamster hearts (Kumagai et al, 1990). Furthermore, in their study Nerves et al showed an Ang(1-7)-induced enhancement of reperfusion arrhythmias in isolated-perfused rat hearts (Neves et al, 1997).…”

Section: Discussionmentioning

confidence: 72%

“…Regarding acute effects of Ang(1-7) Neves et al demonstrated that Ang(1-7) dose-dependently (27-210 nmol L −1 ) diminished coronary flow in isolated-perfused rat hearts, without any changes in inotropy or heart rate (Neves et al, 1997). Kumagai et al observed similar results in isolated hamster hearts (Kumagai et al, 1990).…”

Section: Discussionmentioning

confidence: 88%

Effect of Angiotensin (1-7) on Heart Function in an Experimental Rat Model of Obesity

Blanke¹,

Schlegel²,

Salameh³

et al. 2016

Thorac cardiovasc Surg

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Aim: Obesity is a risk factor for the development of cardiovascular diseases. Recently it was shown that overexpression of the Mas-receptor antagonist angiotensin(1-7) could prevent from diet-induced obesity. However, it remained unclear whether diet-induced obesity and angiotensin(1-7) overexpression might also have effects on the cardiovascular system in these rats.Methods: Twenty three male Sprague Dawley rats were fed with standard chow (SD+chow, n = 5) or a cafeteria diet (SD+CD, n = 6) for 5 months. To investigate the effect of angiotensin(1-7) transgenic rats, expressing an angiotensin(1-7)-producing fusion protein in testis were used. These transgenic rats also received a 5 month's feeding period with either chow (TGR+chow, n = 6) or cafeteria diet (TGR+CD, n = 6), respectively. Hemodynamic measurements (pressure-volume loops) were carried out to assess cardiac function and blood pressure. Subsequently, hearts were explanted and investigated according to the Langendorff technique. Furthermore, cardiac remodeling in these animals was investigated histologically.Results: After 5 months cafeteria diet feeding rats showed a significantly increased body weight, which could be prevented in transgenic rats. However, there was no effect on cardiac performance after cafeteria diet in non-transgenic and transgenic rats. Moreover, overexpression of angiotensin(1-7) deteriorated cardiac contractility as indicated by impaired dp/dt. Furthermore, histological analysis revealed that cafeteria diet led to myocardial fibrosis in both, control and transgenic rats and this was not inhibited by an overproduction of angiotensin(1-7). Conclusion:These results indicate that an overexpression of circulating angiotensin(1-7) prevents a cafeteria diet-induced increase in body weight, but does not affect cardiac performance in this experimental rat model of obesity. Furthermore, overexpression of angiotensin(1-7) alone resulted in an impairment of cardiac function.

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“…Large number of recent discoveries demonstrated the influence of renin-angiotensin system in the mechanism of cardiovascular morbidities that occur due to ischemia reperfusion damage (Linz et al, 1987;Pabla and Curtis, 1995;Neves et al, 1997). The cardiotoxic nature of angiotensin II especially in diabetic cardiovascular diseases are well understood (Hardy et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Pharmacodynamic Interaction of Garlic with Gliclazide and Ramipril on Myocardial Injury in Diabetic Rats

Basheerudd¹

2015

International J. of Pharmacology

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A B S T R A C T Concurrent therapy of herbs with conventional regimen has been extensively reported for alleviating morbidities such as diabetes and cardiac manifestations. One of unique natural remedy that carry abundant potential for ameliorating wide varieties of ailment is garlic. Present research was designed to explore the role of garlic in obviating myocardial dysfunction in diabetic animals, when combined with ramipril and/or gliclazide. Diabetic Sprague Dawley rats received garlic (250 mg kgG 1 ), ramipril (1 mg kgG 1 ) and gliclazide (10 mg kgG 1 ) orally either alone or in combination in their respective groups. At the end of treatment, heart was excised; mounted on modified Langendorff's set-up; perfused with Krebs-Henseleit solution and subjected to ischemia reperfusion injury. Significantly increased percentage recovery was recorded in heart rate and developed tension during post-ischemia in all treated animals, when compared to diabetic group. Further, depleted Superoxide Dismutase (SOD) and catalase activities were substantially inclined in treated groups. Furthermore, combination of garlic with ramipril was most effective in reverting the ischemic damage, whereas, garlic with gliclazide prevent beta cell degeneration. Moreover, histopathological observations validated biochemical and hemodynamic findings. To conclude, this preliminary observation in animals reveals the beneficial role of adding garlic to conventional regimen of gliclazide and ramipril. Further, a careful clinical evaluation of above combination would provide us an opportunity to standardize and implement in cardiovascular disease management in diabetic patient.

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Effect of angiotensin-(1-7) on reperfusion arrhythmias in isolated rat hearts

Cited by 60 publications

References 33 publications

Angiotensin-(1-7) improves the post-ischemic function in isolated perfused rat hearts

Angiotensin-(1-7) improves the post-ischemic function in isolated perfused rat hearts

Effect of Angiotensin (1-7) on Heart Function in an Experimental Rat Model of Obesity

Pharmacodynamic Interaction of Garlic with Gliclazide and Ramipril on Myocardial Injury in Diabetic Rats

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