Effect of androgens on mRNA for a secretory protein of rat dorsolateral prostate and seminal vesicles

Dodd, Janice G.; Kreis, Christophe; Sheppard, Patricia; Hamel, André; Matusik, Robert J.

doi:10.1016/0303-7207(86)90112-7

Cited by 29 publications

(11 citation statements)

References 23 publications

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“…Ventral lobe blots were probed with a gel-purified 420-bp cDNA corresponding to the C, subunit of Prostate Binding Protein (PBP) kindly provided by Dr. R. Matusik (University of Manitoba, Winnepeg, Manitoba, Canada). Lateral and dorsal lobe blots were probed with gel-purified 520-bp probasin cDNA [29,30] and 300-bp RWB cDNA [31], which were also supplied by R. Matusik.…”

Section: Northern Blot Analysismentioning

confidence: 99%

Autologous Regulation of Androgen Receptor Messenger Ribonucleic Acid in the Separate Lobes of the Rat Prostate Gland1

Prins

Woodham

1995

Biology of Reproduction

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Differential autoregulation of androgen receptors (AR) has been previously described for the separate lobes of the rat prostate gland. While AR are up-regulated by testosterone in the ventral, dorsal, and LP1 lateral lobes, the epithelial cells of the LP2 lateral ducts show continued expression of the AR protein following androgen withdrawal. To determine the mechanism of this differential autologous regulation, the present study examined the autoregulation of AR mRNA in the separate regions of the rat prostate gland. Northern blot analysis revealed that AR mRNA levels are down-regulated by androgens in all prostate lobes, since their levels increase following castration and decrease upon testosterone replacement. In situ hybridization confirmed that the increase in AR mRNA levels immediately following androgen withdrawal is due to increased transcripts per cell. When normalized to DNA content, the AR mRNA elevation upon androgen withdrawal was transient, and the value returned to control levels in the ventral and dorsal lobes within three days, while the elevation of AR message in the lateral lobe was prolonged. Quantitative reverse transcriptase-polymerase chain reaction studies revealed that elevated AR mRNA levels in the prolonged absence of androgens were confined to the LP2 ducts of the lateral lobe. Nuclear run-on experiments showed no alteration in AR gene transcription two days after castration in the ventral, dorsal, or LP1 lateral lobes when compared to the values in intact rats, indicating that posttranscriptional mechanisms are involved in AR mRNA autoregulation. In contrast, the AR gene transcription rate doubled in the lateral LP2 ducts. The elevated AR mRNA levels in the LP2 ducts due to increased AR gene transcription following castration may, in part, explain the continued expression of AR protein in that region in the absence of testosterone. However, the mechanism whereby AR translation becomes uncoupled from its AR mRNA levels in the ventral and dorsal lobes after hormone withdrawal remains unclear. In summary, the present data demonstrate that differences exist in AR mRNA regulation within the different regions of the rat prostate gland. These differences may begin to explain differential autoregulation of the AR protein in the separate prostate lobes.

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Section: Northern Blot Analysismentioning

confidence: 99%

Autologous Regulation of Androgen Receptor Messenger Ribonucleic Acid in the Separate Lobes of the Rat Prostate Gland1

Prins

Woodham

1995

Biology of Reproduction

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“…Lobe-specific carbohydrates and glycoproteins are secreted by different prostatic lobes. For example, prostatein (which may be identical to prostatic steroid-binding protein or estramustine-binding protein) is a major secretory glycoprotein in the rat ventral prostate [14][15][16]; two major androgen-dependent secretory glycoproteins, dorsal proteins I (probably identical to a transglutaminase from the coagulating gland) and II, are secreted by both dorsal prostate and coagulating gland [17][18][19][20], whereas two unique small proteins (21 and 23 kDa; also named pM-40) and a protein named seminal vesicle secretion II protein (SVS II or pRWB) are secreted by the dorsolateral prostate [8,[21][22][23]. Additionally, the dorsal prostate is rich in fructose and spermine; the lateral prostate is rich in zinc; the ventral prostate is rich in spermine but contains little carbohydrates; and the coagulating gland secretes large amounts of free fructose and glucose [10,11,14,24,25].…”

Section: Introductionmentioning

confidence: 99%

Comparative study of glycoconjugates of the rat prostatic lobes by lectin histochemistry

Chan

1999

Prostate

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“…To evaluate the secretory function of ventral prostate epithelial cells, we used immunohistochemistry of the prostate-specific secretory protein probasin (Dodd et al, 1986). This protein is particularly highly expressed in rat dorsolateral prostate, but as indicated by immunoblotting using a specific polyclonal antiprobasin antibody, probasin was also expressed in rat and mouse ventral prostates (Fig.…”

Section: Evidence Of Increased Secretion But Not Of Altered Rates Ofmentioning

confidence: 99%

Epithelial Defect in Prostates of Stat5a-Null Mice

Nevalainen

Ahonen

Yamashita

et al. 2000

Laboratory Investigation

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SUMMARY:The transcription factor Stat5a critically mediates prolactin (PRL)-induced mammary gland development and lactogenesis. PRL also stimulates growth and differentiation of prostate tissue. Specifically, hyperprolactinemia gives rise to prostate hyperplasia, and prostate size is reduced in PRL-deficient mice. We therefore investigated the importance of Stat5a for prostate development and function by examining Stat5a-null mice. The absence of Stat5a in mice was associated with a distinct prostate morphology characterized by an increased prevalence of local disorganization within acinar epithelium of ventral prostates. Affected acini were typically filled with desquamated, granular epithelial cells that had become embedded in dense, coagulated secretory material. These features were reminiscent of acinar cyst formation and degeneration frequently observed in human benign prostate hyperplasia, however, cystic changes in prostate acini of Stat5a-deficient mice were not associated with increased prostate size or morphologic hallmarks of epithelial hyperplasia. Instead, immunohistochemistry of the prostate-specific secretory marker, probasin, suggested that hypersecretory function of the epithelium could underlie local congestion and cyst formation in prostates of Stat5a-null mice. Serum testosterone and PRL levels were normal in Stat5a knockout mice, but prostate PRL receptor expression was reduced as determined by immunohistochemistry. Expression levels or activation states of other PRL signal transduction proteins, including Stat5b, Stat3, Stat1, ERK1, and ERK2 were not altered. The present study offers the first evidence for a direct role of Stat5a in the maintenance of normal tissue architecture and function of the mouse prostate. (Lab Invest 2000, 80:993-1006.

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Effect of androgens on mRNA for a secretory protein of rat dorsolateral prostate and seminal vesicles

Cited by 29 publications

References 23 publications

Autologous Regulation of Androgen Receptor Messenger Ribonucleic Acid in the Separate Lobes of the Rat Prostate Gland1

Autologous Regulation of Androgen Receptor Messenger Ribonucleic Acid in the Separate Lobes of the Rat Prostate Gland1

Comparative study of glycoconjugates of the rat prostatic lobes by lectin histochemistry

Epithelial Defect in Prostates of Stat5a-Null Mice

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