Effect of Anandamide on Cytosolic Ca<sup>2+</sup> Levels and Proliferation in Canine Renal Tubular Cells

Yeh, Jeng-Hsien; Cheng, He‐Hsiung; Huang, Chun-Ta; Chung, Hsiao-Min; Chiu, Hui‐Fen; Yang, Yu‐Lin; Yeh, Malcolm; Chen, Wei‐Chuan; Kao, Cheng-Hsing; Chou, Chiang-Ting; Jan, Chung‐Ren

doi:10.1111/j.1742-7843.2006.pto_350.x

Cited by 20 publications

(23 citation statements)

References 35 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 Type 1 and 2 cannabinoid receptors (CB) are bioactive molecules in the Gi/o-protein-coupled receptor family. 9 In experimental animals, increasing CB signaling pathways has been found to induce vasodilation of renal arteries, 10 decrease glomerular filtration rate 11 and contribute to sepsis-induced renal failure. 12 Activation of CB reportedly protects chemotherapy agent cisplatin-induced renal cell apoptotosis 13 and ischemia-mediated tissue injury 14 in mouse kidneys.…”

mentioning

confidence: 99%

Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice

Lei

Shih

Lin

et al. 2015

The American Journal of the Medical Sciences

View full text Add to dashboard Cite

mentioning

confidence: 99%

Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice

Lei

Shih

Lin

et al. 2015

The American Journal of the Medical Sciences

View full text Add to dashboard Cite

“…Maximum and minimum fluorescence values were obtained by adding 0.1% Triton X‐100 (plus 5 mM CaCl 2 ) and 10 mM ethyleneglycoltetraacetic acid sequentially at the end of each experiment. [Ca 2+ ] i was calculated as described previously assuming a K d of 155 nM [16].…”

mentioning

confidence: 99%

Mechanism of Paroxetine‐Induced Cell Death in Renal Tubular Cells

Chen

Huang

et al. 2008

Basic Clin Pharma Tox

Self Cite

View full text Add to dashboard Cite

Paroxetine belongs to the family of selective serotonin reuptake inhibitors. Much research has been performed on the in vitro effect of paroxetine; however, the effect of paroxetine on Madin-Darby canine kidney renal tubular cells is unknown. The present study was aimed at exploring how paroxetine affects viability and to examine the underlying mechanisms. Paroxetine (15-200 μ M) was shown to reduce cell viability via inducing apoptosis in a concentration-dependent manner. Paroxetine-induced cytotoxicity and apoptosis were not changed by the p38 mitogen-activated protein kinase inhibitor SB203580 and the c-Jun NH 2 -terminal kinase inhibitor SP600125, but was potentiated by the extracellular signalregulated kinase inhibitor PD98059; inhibited by GF 109203X, a protein kinase C inhibitor; and potentiated by phorbol 12-myristate 13-acetate, a protein kinase C activator. Paroxetine induced [Ca 2+ ] i rises; however, pre-treatment with 1,2-bis(o-aminophenoxy)ethane-N,N,N ′ ,N ′ -tetraacetic acid tetra(acetoxymethyl)ester, a Ca 2+ chelator, to prevent 20 μ M paroxetineinduced [Ca 2+

show abstract

“…Anandamide previously was shown to increase intracellular Ca 2ϩ levels (Mombouli et al, 1999;Yeh et al, 2006). The changes in intracellular Ca 2ϩ levels could affect the function of Ca 2ϩ -activated Cl Ϫ channels and might interfere with the effect of anandamide on K ϩ currents.…”

Section: Discussionmentioning

confidence: 99%

“…Because anandamide releases intracellular Ca 2ϩ in various cell types (Mombouli et al, 1999;Yeh et al, 2006), activation of Ca 2ϩ -dependent second messenger systems or Ca 2ϩ -activated Cl Ϫ and/or K ϩ channels may interfere with anandamide actions. To examine this possibility, follicle-enclosed oocytes were incubated in 5 M BAPTA-AM for 1 h, and then they were injected with 5 nl of 100 mM BAPTA 10 min before recordings to ensure chelation of intracellular Ca 2ϩ in both follicular cells and oocytes.…”

Section: Fig 2 Anandamide Inhibition Of Cromakalim-activated Kmentioning

confidence: 99%

The Endogenous Cannabinoid Anandamide Inhibits Cromakalim-Activated K⁺Currents in Follicle-EnclosedXenopusOocytes

Öz¹,

Yang²,

Dinç³

et al. 2007

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The effect of the endogenous cannabinoid anandamide on K ϩ currents activated by the ATP-sensitive potassium (K ATP ) channel opener cromakalim was investigated in follicle-enclosed Xenopus oocytes using the two-electrode voltage-clamp technique. Anandamide (1-90 M) reversibly inhibited cromakaliminduced K ϩ currents, with an IC 50 value of 8.1 Ϯ 2 M. Inhibition was noncompetitive and independent of membrane potential. Coapplication of anandamide with the cannabinoid type 1 (, or pertussis toxin (5 g/ml) did not alter the inhibitory effect of anandamide, suggesting that known cannabinoid receptors are not involved in anandamide inhibition of K ϩ currents. Similarly, neither the amidohydrolase inhibitor phenylmethylsulfonyl fluoride (0.2 mM) nor the cyclooxygenase inhibitor indomethacin (5 M) affected anandamide inhibition of K ϩ currents, suggesting that the effects of anandamide are not mediated by its metabolic products. In radioligand binding studies, anandamide inhibited the specific binding of the K ATP ligand [ 3 H]glibenclamide in the oocyte microsomal fractions, with an IC 50 value of 6.3 Ϯ 0.4 M. Gonadotropin-induced oocyte maturation and the cromakalim-acceleration of progesterone-induced oocyte maturation were significantly inhibited in the presence of 10 M anandamide. Collectively, these results indicate that cromakalim-activated K ϩ currents in follicular cells of Xenopus oocytes are modulated by anandamide via a cannabinoid receptor-independent mechanism and that the inhibition of these channels by anandamide alters the responsiveness of oocytes to gonadotropin and progesterone.

show abstract

Effect of Anandamide on Cytosolic Ca²⁺ Levels and Proliferation in Canine Renal Tubular Cells

Cited by 20 publications

References 35 publications

Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice

Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice

Mechanism of Paroxetine‐Induced Cell Death in Renal Tubular Cells

The Endogenous Cannabinoid Anandamide Inhibits Cromakalim-Activated K⁺Currents in Follicle-EnclosedXenopusOocytes

Contact Info

Product

Resources

About

Effect of Anandamide on Cytosolic Ca2+ Levels and Proliferation in Canine Renal Tubular Cells

Cited by 20 publications

References 35 publications

Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice

Induction of Proteinuria by Cannabinoid Receptors 1 Signaling Activation in CB1 Transgenic Mice

Mechanism of Paroxetine‐Induced Cell Death in Renal Tubular Cells

The Endogenous Cannabinoid Anandamide Inhibits Cromakalim-Activated K+Currents in Follicle-EnclosedXenopusOocytes

Contact Info

Product

Resources

About

Effect of Anandamide on Cytosolic Ca²⁺ Levels and Proliferation in Canine Renal Tubular Cells

The Endogenous Cannabinoid Anandamide Inhibits Cromakalim-Activated K⁺Currents in Follicle-EnclosedXenopusOocytes