Effect of anaesthetics MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii)

Abstract

The effects of MS-222 and clove oil on blood biochemical parameters of juvenile Siberian sturgeon (Acipenser baerii) were studied. MS-222 caused higher glucose (GLU) concentrations in anaesthetic test groups than for the control group. Triglyceride (TGL) concentrations of fish in the 140 and 160 mg L )1 groups were also significantly higher than those of other groups. Alanine aminotransferase (ALT) activity in the 140 mg L )1 group was significantly higher than the level in 80, 100 and 120 mg L )1 groups. Aspartate aminotransferase (AST) activity in the 140 mg L )1 group was significantly higher than those in the 100 and 120 mg L )1 groups. Levels of total protein (TP), cholesterol (CHOL) and alkaline phosphatase (ALP) in anaesthetic test groups were not significantly influenced by MS-222. Clove oil did not have significant effects on levels of GLU, TP, CHOL, ALT and ALP. TGL concentration of fish exposed to 180 mg L )1 clove oil was significantly higher than those of the rest anaesthetic groups. AST activities of fish exposed to 120, 150 and 180 mg L )1 were significantly higher than those of 60 and 90 mg L )1 . Overall, TGL and AST could be potentially used as indicators of anaesthetic stress for juvenile Siberian sturgeon. Based on blood biochemical parameters, the appropriate anaesthetic concentrations of MS-222 and clove oil were 80-120 mg L )1 and 60-90 mg L )1 , respectively. Clove oil was a promising alternative to MS-222.

Comparison of Different Anesthetic Protocols for Morphometric Measurements of Carp (Cyprinus carpio)

“…In this study four different group of anesthetics to carp are investigated from the point of view use as an anaesthetizing baths. One of the most widely used anesthetic agents for fish is clove oil, which is readily available, low cost, without prescription and no risk to human health [2]. Our results about clove oil properties such as time of induction and recovery were the same as those described by a number of researchers [11,7,12] and its advantages such as increased safety for fish compared to other immersion drugs [13].…”

“…[1,2,3,4,5]. The most widely method for administration of anesthetics who is analogous to gaseous anesthesia is water-borne anesthesia [6].…”

Anaesthetic efficacy of clove oil, benzocaine, 2-phenoxyethanol and tricaine methanesulfonate in juvenile marbled spinefoot (Siganus rivulatus)

“…Thus, it is of high necessity to establish appropriate anaesthetic concentration to minimize stress (see Feng, Zhuang, Zhang, Kynard, Shi, Liu & Huang 2011). Thus, it is of high necessity to establish appropriate anaesthetic concentration to minimize stress (see Feng, Zhuang, Zhang, Kynard, Shi, Liu & Huang 2011).…”

Reference intervals for select hematologic and plasma biochemical analytes of wild Lake Sturgeon (Acipenser fulvescens) from the St. Lawrence River in New York

“…A previous study found that prolonged handling, analogous to the 24‐hour period that Sturgeon in this study were in the gill nets, resulted in broader intervals for serum analytes than in minimally restrained Adriatic Sturgeon ( Acipenser naccarii ) . Because fish were returned to the river immediately after sampling, and venipuncture in manually restrained Sturgeon has been previously described as a minimally invasive procedure, we chose not to use anesthetics to avoid confounding of intervals, Interestingly, the RIs we report here for values that have reportedly been affected by stress (PCV, sodium and chloride concentrations, and others) are similar to RIs previously reported in anesthetized Sturgeon …”

“…However, the lack of published RIs has prevented the widespread use of these important tools. In addition, many studies have documented the effects of a variety of environmental and physiologic factors, including seasonality, water salinity, temperature and oxygen content, age and sex of the fish, and influence of the use of anesthetics on hematologic and biochemical analytes in fish, thus further complicating a scientifically sound interpretation of these variables . Here, we report hematologic and plasma biochemical values from manually restrained, wild adult Lake Sturgeon.…”