Effect of an electric field during purification of protein using microfiltration

Park, Young G.

doi:10.1016/j.desal.2005.06.044

Cited by 14 publications

(3 citation statements)

References 5 publications

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“…In this case, no significant differences between the fractionation coefficients of any antihypertensive peptides were obtained between voltages of 5-7 V (P=0.05). Similar effects were also observed by Bargeman et al (2002) and Poulin et al (2006) that reported about peptide fractionation out of an αcasein hydrolysate and a β-lactoglobulin hydrolysate with migration rates between 10% and 26% and by Park (2006) for the fractionation of hemoglobin. By increasing the electrical voltage up to 10 V, a decrease in the fractionation coefficient of antihypertensive peptides (S ACE ) was observed (data not shown), which was also reported by Brisson et al (2007) for the separation of lactoferrin at electrical field strengths higher than 1,667 V.m −1 .…”

Section: Determination Of Lactose Modification Via Hplcsupporting

confidence: 85%

Electrochemical modification of dairy-based functional peptides by means of cross-flow electro membrane filtration

Holder

Großmann

Weiß

et al. 2014

Dairy Sci. & Technol.

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International audienceThe stability of functional peptides against electrochemical modification during their selective fractionation via cross-flow electro membrane filtration is a prerequisite for their successful application in food products to ensure the bio-functionality, emulsification, foam formation, or stabilizing properties. This study investigated the impact of electrical fields applied to cross-flow membrane filtration on electrolysis and the associated electrochemical modification of functional dairy-based peptides to calculate the electrical load of target analytes during the process. Firstly, electrolysis and the hypochlorite-induced oxidation of the model peptide β-CN f(108–113) and lactose at different hypochlorite concentrations were studied under well-controlled laboratory conditions, followed by the electrochemical modification of functional peptides during cross-flow electro membrane filtration. At laboratory conditions, a chemical modification of the model peptide β-CN f(108–113) below a value of 3 mol.L−1 hypochlorite was not observed, while lactose was modified at minimal hypochlorite concentrations and, thus, acted as a protection group for peptides. The electrochemical modification of functional peptides was only observed for voltages ≥10 V in cross-flow electro membrane filtration experiments. In addition, results indicate that a further increase in electrical voltage (>5 V) does not result in a significant improvement of peptide electrophoresis, and thus, there is no higher fractionation of functional peptides. These experiments show that under optimal conditions of filtration with a superimposed electrical field, no chemical modification of functional peptides takes place that indicates an advantage compared to conventional filtration processes by significantly increasing the fractionation efficiency at voltages between 5 and 7 V

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Section: Determination Of Lactose Modification Via Hplcsupporting

confidence: 85%

Electrochemical modification of dairy-based functional peptides by means of cross-flow electro membrane filtration

Holder

Großmann

Weiß

et al. 2014

Dairy Sci. & Technol.

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show abstract

“…These effects can contribute to achieve selective extraction. For instance, molecular orientation can enable passage through membrane systems and reduce friction between molecules, and electrochemical reactions can enable molecules to cross boundaries [117,118,121,122]. As for electroosmosis, this principle is applied in CE, where neutral analytes migrate in an electroosmotic flow [123].…”

Section: Electrically Enhanced Sample Preparationmentioning

confidence: 99%

Efficient Discrimination and Removal of Phospholipids During Electromembrane Extraction from Human Plasma Samples

et al. 2017

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“…26 Some investigators have reported signicant enhancements for the removal of arsenic, organic acids, humic acid, biopolymers, and cryptosporidium (a protozoan that causes gastrointestinal illness) with electro-ultraltration or electro-nanoltration. [27][28][29][30][31][32] Other studies have compared the efficacy of continuous and pulsed electric elds for controlling particle deposition and cake formation, 33 or for cleaning fouled membranes. [34][35][36] Therefore, the development of functionalized ultraltration membranes with increased hydrophilicity and electrochemically switchable separation properties could be of great interest.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotube/polyaniline nanofiber ultrafiltration membranes

Liao

Hoek

et al. 2013

J. Mater. Chem. A

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Effect of an electric field during purification of protein using microfiltration

Cited by 14 publications

References 5 publications

Electrochemical modification of dairy-based functional peptides by means of cross-flow electro membrane filtration

Electrochemical modification of dairy-based functional peptides by means of cross-flow electro membrane filtration

Efficient Discrimination and Removal of Phospholipids During Electromembrane Extraction from Human Plasma Samples

Carbon nanotube/polyaniline nanofiber ultrafiltration membranes

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