Effect of an autopolymerizing sealant on viability of microflora in occlusal dental caries

Jensen, Øivind Ekman; Handelman, Stanley L.

doi:10.1111/j.1600-0722.1980.tb01243.x

Cited by 60 publications

(73 citation statements)

References 12 publications

(1 reference statement)

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“…This supports the hypothesis that the microbiota were subjected to a significant nutrient stress with only bacteria able to utilize the limited nutrient source capable of proliferation and survival. The survival, however, may not be long-term since tubular sclerosis and reduced permeability of the dentine (22) will subsequently limit nutrient availability, resulting in further reductions in the numbers of bacteria surviving long-term under restorations (15,16).…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Selection of Microbiota Surviving under Dental Restorations

Paddick

Brailsford

Kidd

et al. 2005

Appl Environ Microbiol

103

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The effects of sealing infected carious dentine below dental restorations on the phenotypic and genotypic diversity of the surviving microbiota was investigated. It was hypothesized that the microbiota would be subject to nutrient limitation or nutrient simplification, as it would no longer have access to dietary components or salivary secretion for growth. The available nutrients would be limited primarily to serum proteins passing from the pulp through the patent dentinal tubules to the infected dentine. Ten lesions were treated, and infected dentine was sealed below dental restorations for approximately 5 months. Duplicate standardized samples of infected dentine were taken at baseline and after the removal of the restorations. The baseline microbiota were composed primarily of Lactobacillus spp., Streptococcus mutans, Streptococcus parasanguinis, Actinomyces israelii, and Actinomyces gerencseriae. None of these taxa were isolated among the microbiota of the dentine samples taken after 5 months, which consisted of only Actinomyces naeslundii, Streptococcus oralis, Streptococcus intermedius, and Streptococcus mitis. The microbiota of the final sample exhibited a significantly (P < 0.001) increased ability to produce glycosidic enzymes (sialidase, ␤-N-acetylglucosaminidase, and ␤-galactosidase), which liberate sugars from glycoproteins. The genotypic diversity of S. oralis and A. naeslundii was significantly (P ‫؍‬ 0.002 and P ‫؍‬ 0.001, respectively) reduced in the final samples. There was significantly (P < 0.001) greater genotypic diversity within these taxa between the pairs of dentine samples taken at baseline than was found in the 5-month samples, indicating that the dentine was more homogenous than it was at baseline. We propose that during the interval between placement of the restorations and their removal, the available nutrient, primarily serum proteins, or the relative simplicity and homogeneity of the nutrient supply significantly affected the surviving microbiota. The surviving microbiota was less complex, based on compositional, phenotypic, and genotypic analyses, than that isolated from carious lesions which were also exposed to salivary secretions and pH perturbations.The survival of bacteria in the mouth and in the oral biofilm, dental plaque, in particular, depends on the ability of the adherent biota obtaining nutrients from their immediate environment and being resistant to fluctuating environmental acid and nutrient stresses (3, 31). Dental plaque rapidly ferments dietary carbohydrates to acids, reversibly demineralizing the underlying enamel, which may ultimately develop into a carious lesion. The fluctuating nature of these acid exposures has significant effects on the microbiota of the oral biofilm (25). Perhaps the best documented are the increased representation of yeasts, lactobacilli, and mutans streptococci in dental plaque and saliva, especially of individuals with high caries scores and xerostomia (2, 20, 21). Other investigators have studied the effects of acids on the oral flor...

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Section: Discussionmentioning

confidence: 99%

Phenotypic and Genotypic Selection of Microbiota Surviving under Dental Restorations

Paddick

Brailsford

Kidd

et al. 2005

Appl Environ Microbiol

103

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“…Since cavity preparation usually involves some loss of healthy dental tissue, alternative approaches have been proposed to preserve tooth structure, such as the sealing of carious tissue beneath sealants or restorations. These approaches may even reduce or completely eliminate the population of viable microorganisms, 1,2,3,4,5,6,7,8 thus controlling caries progression.…”

Section: Introductionmentioning

confidence: 99%

A randomized clinical trial on the sealing of occlusal carious lesions: 3–4-year results

et al. 2017

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This randomized clinical trial aimed to assess the efficacy of sealing occlusal carious lesions in permanent teeth. The sample consisted of 54 occlusal carious lesions in permanent molars and premolars of 49 patients aged 8-43 years (median: 19 years). The inclusion criteria comprised the presence of a cavity with no access allowing biofilm control. The maximum depth of the lesion was the middle third of the dentin thickness, as assessed by bitewing radiography. The teeth were randomly assigned to sealant treatment (n = 28) or restorative treatment (n = 26). Clinical and radiographic examinations were performed after 1 year and after 3-4 years. The outcomes depended on the clinical performance of the sealant/restoration and the control of caries progression observed radiographically. Survival analysis was performed to assess success rates. Over the 3-4 years of monitoring, 2 sealants were totally lost, 1 needed repair, and 1 showed caries progression, totaling 4 failures in the sealant group. In the restoration group, 1 failure was observed (in need of repair). The success rates were 76% and 94% in the sealant and the restoration groups, respectively (p > 0.05). The sealing of occlusal carious lesions in permanent teeth succeeded in controlling caries over a 3-4-year period. However, sealed carious lesions require patient compliance in attending regular follow-ups to control the occurrence of clinical failures of the sealants.

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“…Sin embargo, es posible que los dientes con lesiones de la dentina bajo superficies de esmalte aparentemente sanas (caries ocultas) sean equivocadamente sellados 13 . El sellado marginal es importante para el éxito del sellador porque la penetración de las bacterias bajo el sellador podría permitir la aparición de caries y/o la progresión de ésta 14,15 .…”

Section: Discussionunclassified

Efecto del fotocurado con luz led en la filtración marginal de un sellante de fosas y fisuras

Mejia

Zevallos

Hoyos

2014

Odontol. sanmarquina

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El propósito de éste estudio fue evaluar in vitro el efecto del fotocurado con luz emitida por diodos L.E.D. y luz halogena convencional en la filtracion marginal de un sellante de fosas y fisuras. Se emplearon 40 premolares permanentes libres de caries, recientemente extraídas, las que fueron aleatoriamente distribuidas en dos grupos (n=20), se les colocó sellante de fisura a base de resina, Helioseal F (Ivoclar Vivadent), de acuerdo a los patrones establecidos, fotopolimerizados ya sea con luz L.E.D. o luz halogena convencional (grupo control). Los especímenes fueron colocados en agua destilada a 37º C por 48 horas, luego se cubrieron todas las superficies dentarias con esmalte para uñas excepto un margen de 2 mm alrededor de los sellantes, se hizo el proceso de termociclado de las muestras, utilizando una solución de azul de metileno al 1% usado como indicador de la filtración marginal, el regimen fue de 500 ciclos entre 5° y 60°C. Luego los dientes fueron seccionados en sentido vestibulo lingual, para evaluar la filtracion marginal en un microscopio estereoscópico. El 35% y 60% de los sellantes fotopolimerizados con luz L.E.D. y luz halógena respectivamente presentaron algún grado de filtración marginal, pero ésta diferencia no mostró significancia estadística (p>0.05). El fotocurado con luz L.E.D. o con luz halogena convencional tienen efectos similares sobre el grado de filtracion marginal de un sellante de fosas y fisuras

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Effect of an autopolymerizing sealant on viability of microflora in occlusal dental caries

Cited by 60 publications

References 12 publications

Phenotypic and Genotypic Selection of Microbiota Surviving under Dental Restorations

Phenotypic and Genotypic Selection of Microbiota Surviving under Dental Restorations

A randomized clinical trial on the sealing of occlusal carious lesions: 3–4-year results

Efecto del fotocurado con luz led en la filtración marginal de un sellante de fosas y fisuras

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