Effect of ampicillin, cefmetazole and minocycline on the adherence of Branhamella catarrhalis to pharyngeal epithelial cells.

Abstract

of Ampicillin, Cefmetazole and Minocycline on the Adherence of Branhamella catarrhalis to Pharyngeal Epithelial Cells. Tohoku J. Exp. Med., 1990, 161(1),1-7Using pharyngeal epithelial cells from a healthy adult and eight strains of Branhamella catarrhalis (B. catarrhalis) isolated from eight patients with respiratory infection the effect of subminimal inhibitory concentrations of cefmetazole, ampicillin and minocycline on adherence was examined. Cefmetazole-treated bacterial attachment (44 + 28; mean ± s.D.) decreased significantly (p <0.05) compared to the control (84+27). Statistically no significant difference in adherence was found between ampicillin-treated bacteria (63 + 36) and the control (95 + 40) or minocycline-treated bacteria (91 + 39) and the control (109+40). Large bacteria was observed after cefmetazole and ampicillin treatment. In addition to diplococci, tetrads were observed after cefmetazole treatment. Significant correlation between the MICs and adherence ability was not found. The results suggests that these three antibiotics were not responsible for the increase in B. catarrhalis infection by increasing adherence ability.

Ultrastructural Study on the Adherence of Branhamella catarrhalis to Oropharyngeal Epithelial Cell

“…Evidence has shown that respiratory pathogens first attach to the pharyngeal cells, then according to its virulence, tissue tropism and host resistance, causes different types of respiratory injections. Several important events of adherence of B. catarrhalis have been reported from our department (1,12,15,16) with the aim to identifying the adhesin and receptor.…”

Causative organisms of acute respiratory infections in northern Thailand.

“…Subsequently we reported various important aspect of this bacteria to elucidate its mechanism of pathogenicity (Ahmed et al, 1990; Rikitomi et at., 1991; Ahmed et al, 1992). In this study we found in Mae Sot, Thailand, B. catarrhalis is one of the major pathogen in respiratory infection.…”