Effect of amoxycillin and doxycycline on function of human granulocytes tested in vitro and on chemotaxis of granulocytes from rabbits given the two antibiotics

Abstract

The effect of amoxycillin and doxycycline on human granulocyte function was studied in vitro. The antimicrobial agents were added to tests of chemotaxis, random motility, yeast phagocytosis, and killing, at progressing concentrations. The effect was also evaluated in vivo by measuring chemotaxis and random motility (agarose technique) of granulocytes from rabbits injected with these antibiotics. Amoxycillin was shown to have a slightly stimulating effect on chemotaxis, demonstrable only at the highest concentration tested (100 micrograms/ml), and no effect on the other variables. Doxycycline had a dose-related inhibitory effect on chemotaxis, random motility, and phagocytosis, and no effect on killing. Chemotaxis and random motility were slightly, but not significantly, stimulated in vivo when rabbits were given amoxycillin. Chemotaxis (but not random motility) was significantly impaired when the rabbits were given doxycycline.

The effects of some antibiotics on polymorphonuclear leukocyte functions of elderly patients in vitro before and after zinc supplementation

“…Crec et al [23] reported that doxycycline (2, 10 and 100 Ag/ml) had an inhibitory effect on chemotaxis ( p b 0.001), random motility ( p b 0.05) and phagocytosis ( p b 0.001) of granulocytes from rabbits, but it did not effect yeast killing ( p N 0.05).…”

Treatment of category III A prostatitis with zafirlukast: a randomized controlled feasibility study

“…For a significant leucocytosis in the post-prostatic massage urine, the figures were three of 10 (30%) and three of seven (43%) for zafirlukast and placebo, respectively. The number of days (mean, SD and range) between initial and follow-up visits was 32.3 (2.5) (29)(30)(31)(32)(33)(34)(35)(36) for the actively treated group and 35.7 (15.3) (228-270) for the placebo arm.…”

“…However, there is some subjective, clinical non-placebo-controlled evidence that doxycycline has a beneficial outcome in the treatment of prostatitis from at least two studies. 25,30 Furthermore, a number of in vitro studies show that tetracyclines, particularly doxycycline, have significant effects on impairing polymorph function, [31][32][33][34][35] macrophage/monocyte cell function 36,37 suppressing delayed hypersensitivity reaction 38 and generally inhibiting mammalian cell proliferation and migration. 39 Additionally, many patients with chronic prostatitis/chronic pelvic pain syndrome have a wide variety of bacterial DNA encoding sequences, 75% of which are tetracycline sensitive, despite extensive negative microbiological investigations.…”