Effect of Amniotic Fluid Stem Cells and Amniotic Fluid Cells on the Wound Healing Process in a White Rat Model

Abstract

BackgroundAmniotic-fluid-derived stem cells and amniocytes have recently been determined to have wound healing effects, but their mechanism is not yet clearly understood. In this study, the effects of amniotic fluid stem cells and amniocytes on wound healing were investigated through animal experiments.MethodsOn the back of Sprague-Dawley rats, four circular full-thickness skin wounds 2 cm in diameter were created. The wounds were classified into the following four types: a control group using Tegaderm disc wound dressings and experimental groups using collagen discs, amniotic fluid stem cell discs, and amniocyte discs. The wounds were assessed through macroscopic histological examination and immunohistochemistry over a period of time.ResultsThe amniotic fluid stem cell and amniocyte groups showed higher wound healing rates compared with the control group; histologically, the inflammatory cell invasion disappeared more quickly in these groups, and there was more significant angiogenesis. In particular, these groups had significant promotion of epithelial cell reproduction, collagen fiber formation, and angiogenesis during the initial 10 days of the wound healing process. The potency of transforming growth factor-β and fibronectin in the experimental group was much greater than that in the control group in the early stage of the wound healing process. In later stages, however, no significant difference was observed.ConclusionsThe amniotic fluid stem cells and amniocytes were confirmed to have accelerated the inflammatory stage to contribute to an enhanced cure rate and shortened wound healing period. Therefore, they hold promise as wound treatment agents.

Inhibition of glycogen synthase kinase-3 (GSK3) promotes the neural differentiation of full-term amniotic fluid-derived stem cells towards neural progenitor cells

“…This result suggested that CD117-positive cells exhibited mesenchymal characteristics. Moreover, consistent with our results, other studies reported that hAFS cells can express the stem cell markers CD13, CD29, CD44, CD73, CD90, CD105 and CD117 (Davydova et al, 2009;Wouters et al, 2007;Yang et al, 2013;Zagoura et al, 2013). Additionally, we found that CD117-positive hAFS cells expressed the transcription factor OCT4, which works as a key factor in the transcriptional network to govern pluripotency and self-renewal of ESCs.…”

Hypoxic Conditioned Medium from Human Amniotic Fluid-Derived Mesenchymal Stem Cells Accelerates Skin Wound Healing through TGF-β/SMAD2 and PI3K/Akt Pathways

“…MSCs have great potential in the fields of tissue engineering and regenerative medicine [38]. Recent studies have focused on the effect of reducing oxygen tension (hypoxia) on the behavior and functions of MSCs.…”

Problems Associated with Alloplastic Materials in Rhinoplasty

“…There are reports that adipose-derived stem cell therapy was used to treat nasal skin necrosis, and satisfactory results of healing were obtained. 34 , 35 , 36 , 37 , 38 …”