Effect of ammonia on the photosynthetic activity of Arthrospira and Chlorella: A study on chlorophyll fluorescence and electron transport

Μάρκου, Γιώργος; Depraetere, Orily; Muylaert, Koenraad

doi:10.1016/j.algal.2016.03.039

Cited by 135 publications

(50 citation statements)

References 52 publications

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“…In order to answer this question, chlorophyll fluorescence analysis was employed and maximum quantum yield (QY) of the Photosystem II (PSII) was recorded, which is a quantifiable indicator of the efficiency of PSII photochemistry [24]. As shown in Table 1, the QY of A. platensis did not change much (0.48-0.56) between the experimental series, which suggest that A. platensis was not directly inhibited by the addition of glycerol, as any kind of inhibition would have been indicated by a significant decrease in the QY (values less than 0.4) [25][26][27]. Moreover, the pH of the cultures was almost the same in all experimental series ranging from 9.8-10.1, indicating that the pH of the growth medium was neither a cause for the biomass concentration reduction.…”

Section: Effect Of Light and Glycerol Concentration On The Growth Permentioning

confidence: 85%

Effect of Glycerol Concentration and Light Intensity on Growth and Biochemical Composition of Arthrospira (Spirulina) Platensis: A Study in Semi-Continuous Mode with Non-Aseptic Conditions

et al. 2019

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In this study, Arthrospira platensis was grown in the presence of different glycerol concentrations (0.5–9 g/L) under three light intensities (5, 10 and 15 Klux) in semi-continuous mode and under non-axenic conditions. The aim of this study was to investigate the growth performance, the biomass biochemical composition and any interactions between A. platensis and bacteria that would potentially grow as well on glycerol. The results here show that glycerol did not have any positive effect on biomass production of A. platensis. In contrast, it was observed that by increasing glycerol concentration the growth performance of A. platensis was restricted, while a gradual increase of bacteria population was observed, which apparently outcompeted and repressed A. platensis growth. Chlorophyll fluorescence measurements (Quantum Yields) revealed that glycerol was not an inhibiting factor per se of photosynthesis. On the other hand, cyanobacterial biomass grown on glycerol displayed a higher content in proteins and lipids. Especially, protein productivity was enhanced around 15–35% with the addition of glycerol compared to the control. In distinction, carbohydrate and photosynthetic pigments (phycocyanin and chlorophyll-α) content decreased with the increase of glycerol concentration. The results here suggest that A. platensis did not utilize glycerol for biomass production but most probably as metabolic energy carrier towards synthesis of proteins and lipids, which are more energy consuming metabolites compared to carbohydrates. The study revealed that the addition of glycerol at amounts of 0.5–1.5 g/L could be a strategy to improve protein productivity by A. platensis.

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Section: Effect Of Light and Glycerol Concentration On The Growth Permentioning

confidence: 85%

Effect of Glycerol Concentration and Light Intensity on Growth and Biochemical Composition of Arthrospira (Spirulina) Platensis: A Study in Semi-Continuous Mode with Non-Aseptic Conditions

et al. 2019

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“…Chlorophyll fluorescence is a widely used technique to measure the photosynthetic performance (mainly PS-II activity) because it is easy, fast, non-invasive, and provides plenty of information about the fundamental performance of the PS-II of the cells under various environmental conditions [38]. The parameter F v /F m represents a maximum potential quantum efficiency of PS-II, if all the reaction centers were open.…”

Section: Resultsmentioning

confidence: 99%

Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus

Chokshi

Pancha

Ghosh

et al. 2017

Biotechnol Biofuels

171

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BackgroundMicroalgae accumulate a considerable amount of lipids and carbohydrate under nutrient-deficient conditions, which makes them one of the promising sustainable resources for biofuel production. In the present study, to obtain the biomass with higher lipid and carbohydrate contents, we implemented a short-term nitrogen starvation of 1, 2, and 3 days in a green microalga Acutodesmus dimorphus. Few recent reports suggest that oxidative stress-tolerant microalgae are highly efficient for biofuel production. To study the role of oxidative stress due to nitrogen deficiency, responses of various stress biomarkers like reactive oxygen species (ROS), cellular enzymatic antioxidants superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and non-enzymatic scavengers proline and polyphenols were also evaluated. Further, the endogenous levels of phytohormones abscisic acid (ABA) and indole-3-acetic acid (IAA) were also determined to study their response to nitrogen deficiency.ResultsWe observed that nitrogen starvation of 2 days is effective to produce biomass containing 29.92% of lipid (comprising about 75% of neutral lipid) and 34.80% of carbohydrate, which is significantly higher (about 23 and 64%, respectively) than that of the control culture. Among all nitrogen-starved cultures, the accumulations of ROS were lower in 2 days starved culture, which can be linked with the several folds higher activities of SOD and CAT in this culture. The accumulations of proline and total polyphenols were also significantly higher (about 4.7- and 1.7-folds, respectively, than that of the control) in 2 days nitrogen-starved culture. The levels of phytohormones once decreased significantly after 1 day, increased continuously up to 3 days of nitrogen starvation.ConclusionThe findings of the present study highlight the interaction of nitrogen starvation-induced oxidative stress with the signaling involved in the growth and development of microalga. The study presents a comprehensive picture of the adaptive mechanisms of the cells from a physiological perspective along with providing the strategy to improve the biofuel potential of A. dimorphus through a short-term nitrogen starvation.

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“…Chlorophyll fluorescence was measured according to Markou et al . using the portable PAM fluorometer AquaPen‐C AP‐C 100 (Photon Systems Instruments, Drassov, Czech Republic).…”

Section: Methodsmentioning

confidence: 99%

Treatment of real aquaculture wastewater from a fishery utilizing phytoremediation with microalgae

Liu

Feng

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND: In this study, five microalgal species (Chlorella vulgaris, Chlorococcum sp. GD, Parachlorella kessleri TY, Scenedesmus quadricauda, and Scenedesmus obliquus) were cultivated in batch mode to evaluate their respective potential for the treatment of real aquaculture wastewater from a fishery. Subsequently, the microalga with the best performance was cultivated with different initial inoculation concentrations to evaluate the effect of initial inoculation on pollutant removal efficiency. RESULTS: When real aquaculture wastewater was inoculated with exogenous microalgae, the growth of both indigenous microalgae and bacteria was significantly inhibited. Pollutant removal was closely related to exogenous inoculationof microalgae. Parachlorella kessleri TY had high growth potential and pollutant removal capability in aquaculture wastewater, compared with the other four microalgae. When the wastewater was inoculated with low biomass concentrations of P. kessleri TY (50-100 mg L −1 ), it grew well and degraded most of the encountered pollutant. In particular, P. kessleri TY with 100 mg L −1 of inoculation concentration removed 94.4% of COD, 96.2% of ammonium, 99% of nitrite, 94.3% of nitrate, and 95.6% of phosphorus after 3 days of cultivation. CONCLUSIONS: Both the screening for microalgal species and the regulation of initial inoculation concentrations are promising approaches to enhance pollutant removal efficiency from real aquaculture wastewater. Determination of water qualityTo determine the water quality, the microalgal suspension was centrifuged at 5000 rpm for 5 min and the supernatant J Chem Technol Biotechnol 2019; 94: 900-910 /jctb up to 94.4%, 96.2%, 99%, 94.3%, and 95.6% for COD, ammonium, nitrite, nitrate, and total phosphorus, respectively. In the remaining tested inoculation concentrations (25, 50, 200, and 400 mg L −1 ), 77.8-88.9% of COD, 54.2-86.1% of ammonium, 64.1-91.7% of nitrite, 57.1-85.7% of nitrate, and 53-81.4% of total phosphorus were removed from the aquaculture wastewater J Chem Technol Biotechnol 2019; 94: 900-910

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Effect of ammonia on the photosynthetic activity of Arthrospira and Chlorella: A study on chlorophyll fluorescence and electron transport

Cited by 135 publications

References 52 publications

Effect of Glycerol Concentration and Light Intensity on Growth and Biochemical Composition of Arthrospira (Spirulina) Platensis: A Study in Semi-Continuous Mode with Non-Aseptic Conditions

Effect of Glycerol Concentration and Light Intensity on Growth and Biochemical Composition of Arthrospira (Spirulina) Platensis: A Study in Semi-Continuous Mode with Non-Aseptic Conditions

Nitrogen starvation-induced cellular crosstalk of ROS-scavenging antioxidants and phytohormone enhanced the biofuel potential of green microalga Acutodesmus dimorphus

Treatment of real aquaculture wastewater from a fishery utilizing phytoremediation with microalgae

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