Effect of ammonia on anaerobic digestion of municipal solid waste: Inhibitory performance, bioaugmentation and microbiome functional reconstruction

Miao, Yanqing; Treu, Laura; Campanaro, Stefano; Tian, Hailin; Zhu, Xinyu; Khoshnevisan, Benyamin; Tsapekos, Panagiotis; Angelidaki, Irini; Fotidis, Ioannis A.

doi:10.1016/j.cej.2020.126159

Cited by 81 publications

(23 citation statements)

References 60 publications

(69 reference statements)

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“… According to the pathways present in these Bacteria , they are capable of performing a complete fermentation, converting glucose to acetate via the Embden–Meyerhof pathway and pyruvate oxidation (Table S5). These results show consistency with previous findings which suggested the main driving forces expanding the complexity and stability of the AD microbiome . Meanwhile, Peptococcaceae sp.…”

Section: Results and Discussionsupporting

confidence: 92%

“…These results show consistency with previous findings which suggested the main driving forces expanding the complexity and stability of the AD microbiome. 58 Meanwhile, Peptococcaceae sp. DTU890 was also involved in acetogenesis using a novel glycine cleavage system and the phosphate acetyltransferase–acetate kinase pathway ( Figure 3 ).…”

Section: Results and Discussionmentioning

confidence: 99%

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Insights into Ammonia Adaptation and Methanogenic Precursor Oxidation by Genome-Centric Analysis

Miao

Treu

Zhu

et al. 2020

Environ. Sci. Technol.

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Ammonia released from the degradation of protein and/or urea usually leads to suboptimal anaerobic digestion (AD) when N-rich organic waste is used. However, the insights behind the differential ammonia tolerance of anaerobic microbiomes remain an enigma. In this study, the cultivation in synthetic medium with different carbon sources (acetate, methanol, formate, and H 2 /CO 2 ) shaped a common initial inoculum into four unique ammonia-tolerant syntrophic populations. Specifically, various levels of ammonia tolerance were observed: consortia fed with methanol and H 2 /CO 2 could grow at ammonia levels up to 7.25 g NH + -N/L, whereas the other two groups (formate and acetate) only thrived at 5.25 and 4.25 g NH + -N/L, respectively. Metabolic reconstruction highlighted that this divergent microbiome might be achieved by complementary metabolisms to maximize biomethane recovery from carbon sources, thus indicating the importance of the syntrophic community in the AD of N-rich substrates. Besides, sodium/proton antiporter operon, osmoprotectant/K + regulator, and osmoprotectant synthesis operon may function as the main drivers of adaptation to the ammonia stress. Moreover, energy from the substrate-level phosphorylation and multiple energy-converting hydrogenases ( e.g. , Ech and Eha) could aid methanogens to balance the energy request for anabolic activities and contribute to thriving when exposed to high ammonia levels.

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Section: Results and Discussionsupporting

confidence: 92%

Section: Results and Discussionmentioning

confidence: 99%

Insights into Ammonia Adaptation and Methanogenic Precursor Oxidation by Genome-Centric Analysis

Miao

Treu

Zhu

et al. 2020

Environ. Sci. Technol.

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“…Acclimation of the anaerobic microflora allows digestion to proceed under extreme ammonia levels, as demonstrated by Yan et al [59], who reported the treatment of the OFMSW at an ammonia content of 9.5 g/L. Bioaugmentation with a pure culture of Methanoculleus bourgensis allowed digestion to proceed at 13.5 g NH 4 + /L [60]. The experiences regarding digestion performance under such severe conditions seem extremely interesting as academy activity.…”

Section: Increasing Biomass Activity As a Strategy For Improving Performancementioning

confidence: 96%

Biogas Production from Organic Wastes: Integrating Concepts of Circular Economy

Ellacuriaga

García-Cascallana

Gómez

2021

Fuels

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Anaerobic digestion is traditionally used for treating organic materials. This allows the valorization of biogas and recycling of nutrients thanks to the land application of digestates. However, although this technology offers a multitude of advantages, it is still far from playing a relevant role in the energy market and from having significant participation in decarbonizing the economy. Biogas can be submitted to upgrading processes to reach methane content close to that of natural gas and therefore be compatible with many of its industrial applications. However, the high installation and operating costs of these treatment plants are the main constraints for the application of this technology in many countries. There is an urgent need of increasing reactor productivity, biogas yields, and operating at greater throughput without compromising digestion stability. Working at organic solid contents greater than 20% and enhancing hydrolysis and biogas yields to allow retention times to be around 15 days would lead to a significant decrease in reactor volume and therefore in initial capital investments. Anaerobic digestion should be considered as one of the key components in a new economy model characterized by an increase in the degree of circularity. The present manuscript reviews the digestion process analyzing the main parameters associated with digestion performance. The novelty of this manuscript is based on the link established between operating reactor conditions, optimizing treatment capacity, and reducing operating costs that would lead to unlocking the potential of biogas to promote bioenergy production, sustainable agronomic practices, and the integration of this technology into the energy grid.

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“…This is consistent with a previous study where dead cells led to an increase of ammonia when changed into a new environment (Ma et al, 2019). During the whole experiment, the total nitrogen removal efficiency did not exceed 10%, indicating that the anaerobically digested sludge had a limited ability to remove ammonia nitrogen from wastewater (Yan et al, 2020). Therefore, a subsequent autotrophic nitrogen removal process is still needed.…”

Section: Resultsmentioning

confidence: 99%

Comparison of three anaerobic digestion reactors for low‐carbon wastewater treatment

Wei

Zhang

Zhao

et al. 2022

Water Environment Research

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In this study, three anaerobic digestion reactors using up‐flow anaerobic sludge blanket (UASB), anaerobic sequencing batch reactor (AnSBR), and anaerobic membrane bioreactor (AnMBR) were studied. The chemical oxygen demand (COD), gas production, sludge performance, and microbial characteristics of the anaerobic digestion process were assessed. The results showed that the average COD removal efficiencies reached 86%, 83%, and 85%, with corresponding removed rates of 2.49, 0.48, and 0.79 kg COD m−3 d−1 in UASB, AnSBR, and AnMBR, respectively. After the reactors attained stable operation, both extracellular polymeric substances and soluble microbial products decreased in all the reactors compared with the seed sludge. Methanothrix was the dominant archaea for methane production in the UASB, the relative abundance of which increased from 58.3% to 83.4%. These results identify UASB as the most suitable reactor for anaerobic digestion when treating wastewater with low carbon. Such reactors are important for the application and development of the energy self‐sufficiency in sewage treatment. Practitioner Points UASB, SBR, and MBR were adopted to treat low‐carbon wastewater using anaerobic digestion process. UASB performed the highest COD removal from low‐carbon wastewater. The main microorganisms in UASB were Methanothrix, Methanomassiliicoccus, and Methanobacterium.

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Effect of ammonia on anaerobic digestion of municipal solid waste: Inhibitory performance, bioaugmentation and microbiome functional reconstruction

Cited by 81 publications

References 60 publications

Insights into Ammonia Adaptation and Methanogenic Precursor Oxidation by Genome-Centric Analysis

Insights into Ammonia Adaptation and Methanogenic Precursor Oxidation by Genome-Centric Analysis

Biogas Production from Organic Wastes: Integrating Concepts of Circular Economy

Comparison of three anaerobic digestion reactors for low‐carbon wastewater treatment

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