Effect of Aminoguanidine on Erythrocyte Lipid Peroxiclation and Activities of Antioxidant Enzymes in Experimental Diabetes

Kędziora–Kornatowska, Kornelia; Luciak, M; Błaszczyk, Jan; Pawlak, Wojciech

doi:10.1515/cclm.1998.137

Cited by 48 publications

(37 citation statements)

References 40 publications

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“…Similar finding were reported by Kedziora -Kornatowska KZ. 28 Our results are in contrast with findings of Ceriello et al 29 and Selvum et al 30 wherein significantly increased catalase activity in tissues and blood of diabetics was reported compared to controls.…”

Section: Overproduction Of Free Radicals and Possible Metabolic Intercontrasting

confidence: 99%

Oxidative Stress in Patients with Diabetes Mellitus

Palekar¹,

Ray²

2016

JDMDC

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Oxidative stress in a biological system is the shift in the homeostasis between oxidants and antioxidants in favour of oxidants. It is suggested to be playing a key role in onset and development of complication of Diabetes Mellitus. The present study aimed to assess the oxidative stress and compare the antioxidant enzyme status in patients with Type 2 Diabetes and paired controls. Total study population consisted of 33 subjects with Diabetes and 10 controls from both male and female gender incorporated for the study. Plasma Malondialdehyde level, an indicator of oxidative stress and antioxidative enzyme system including Erythrocyte Superoxide dismutase, catalase and Glutathione Peroxidase were estimated. Subjects with diabetes had significantly higher plasma malondialdehyde as compared to controls. Antioxidant enzymes levels were found to be lower in diabetic patients as compared to controls. In conclusion, hyperglycemia increases production of free radicals leading to increased lipid peroxidation. The antioxidant enzyme system weakens in diabetic patients compared to controls. It was noted that patients with good glycemic control had less oxidative stress compared to patients with poor control.

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Section: Overproduction Of Free Radicals and Possible Metabolic Intercontrasting

confidence: 99%

Oxidative Stress in Patients with Diabetes Mellitus

Palekar¹,

Ray²

2016

JDMDC

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“…The same effect on these enzymes was observed in diabetic rats [29] and on superoxide dismutases in doxorubicin treated rats [30] . GPx activity was slightly decreased after AG treatment in a model of liver injury in rat [31] .…”

Section: Discussionsupporting

confidence: 74%

Aminoguanidine impedes human pancreatic tumor growth and metastasis development in nude mice

Mohamad¹,

Cricco²,

Sambuco³

et al. 2009

WJG

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AIM:To study the action of aminoguanidine on pancreatic cancer xenografts in relation to cell proliferation, apoptosis, redox status and vascularization. METHODS:Xenografts of PANC-1 cells were developed in nude mice. The animals were separated into two groups: control and aminoguanidine treated. Tumor growth, survival and appearance of metastases were determined in vivo in both groups. Tumors were excised and ex vivo histochemical studies were performed. Cell growth was assessed by Ki-67 expression. Apoptosis was studied by intratumoral expression of B cell lymphoma-2 protein (Bcl-2) family proteins and Terminal deoxynucleotidyl transferase biotin-dUTP Nick End Labeling (Tunel). Redox status was evaluated by the expression of endothelial nitric oxide synthase (eNOS), catalase, copper-zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutase (MnSOD) and glutathione peroxidase (GPx). Finally, vascularization was determined by Massons trichromic staining, and by VEGF and CD34 expression. RESULTS:Tumor volumes after 32 d of treatment by aminoguanidine (AG) were significantly lower than in control mice (P < 0.01). Median survival of AG mice was significantly greater than control animals (P < 0.01). The appearance of both homolateral and contralateral palpable metastases was significantly delayed in AG group. Apoptotic cells, intratumoral vascularization (trichromic stain) and the expression of Ki-67, Bax, eNOS, CD34, VEGF, catalase, CuZnSOD and MnSOD were diminished in AG treated mice (P < 0.01), while the expression of Bcl-2 and GPx did not change. C O N C L U S I O N :T h e a n t i t u m o r a l a c t i o n o f aminoguanidine is associated with decreased cell proliferation, reduced angiogenesis, and reduced expression of antioxidant enzymes.

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“…Highly reactive free radicals under oxidative stress interact with a variety of biomolecules like polyunsaturated fatty acids, 42 pancreas, 43 blood, [44][45][46] and red blood cells, 47 whereas decreased activity was seen in the heart 48,49 and retina. 50 The effect of diabetes on the activity of SOD was erratic, with no discernable pattern based on gender or species of animal, or duration of diabetes or tissue studied.…”

Section: Discussionmentioning

confidence: 99%