INFORMATION TO USERSThis reproduction was made from a copy of a document sent to us for microfilming. While the most advanced technology has been used to photograph and reproduce this document, the quality of the reproduction is heavily dependent upon the quality of the material submitted.The following explanation of techniques is provided to help clarify markings or notations which may appear on this reproduction. 1.The sign or "target" for pages apparently lacking from the document photographed is "Missing Page(s)". If it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting through an image and duplicating adjacent pages to assure complete continuity.2. When an image on the film is obliterated with a round black mark, it is an indication of either blurred copy because of movement during exposure, duplicate copy, or copyrighted materials that should not have been filmed. For blurred pages, a good image of the page can be found in the adjacent frame. If copyrighted materials were deleted^ a target note will appear listing the pages in the adjacent frame.3. When a map, drawing or chart, etc., is part of the material being photographed, a definite method of "sectioning" the material has been followed. It is customary to begin filming at the upper left hand comer of a large sheet an^d to continue from left to right in equal sections with small overlaps. If necess^py, sectioning is continued again-beginning below the first row and continuing on until complete. SUMMARY 36 APPENDIX 86 1 LITERATURE REVIEW Indispensable Amino Acid Requirements Based on innovative pioneer work. Rose (1938) classified arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine as indispensable amino acids for the rat. Rose defined an indispensable amino acid as one that cannot be synthesized in the body at a rate required for normal growth. The investigations with rats stimulated work with other species. Swine were found to require the same 10 amino acids for normal growth. The laboratories of J. K. Loosli at Cornell University (Bell, 1948; Brinegar, 1951) and W. M. Beeson at Purdue University (Beeson et al., 1948; Mertz et al., 1949; Shelton et al., 1950) were the first to conduct qualitative and preliminary quantitative amino acid requirement studies with pigs (Baker and Speer, 1983). Subsequently, Becker et al.(1963) estimated quantitative requirements of all indispensable amino acids for swine at various ages. For the weanling pig (13.6 kg), amino acid needs increased as protein level in the diet increased. His calculations were based on the assumption that the requirements for all amino acids, expressed as a percentage of the protein, decrease linearly as the dietary protein increases. However, NRC (1979) bases indispensable amino acid requirements on a constant percentage of the protein with advancing age and weight, because the amino acid concentrations in lean tissue remain the same and ma...