Effect of aluminum on the in vitro activity of acid phosphatases of four potato clones grown in three growth systems

Abstract

The aim of this study was to assess the effect of aluminum on the in vitro activity of acid phosphatases (APases) of four potato clones, Macaca and Dakota Rose (Al-sensitive), and SMIC148-A and Solanum microdontum (Al-tolerant), grown in vitro, in hydroponics or in a greenhouse. The enzyme was assayed in vitro in the presence of 0, 1.85, 3.70, 5.55 and 7.40 mM Al. In plantlets grown in vitro, root APases were inhibited by Al in all clones, while shoot APases were inhibited by Al in S. microdontum and Dakota Rose and increased in Macaca at all Al concentrations. In plantlets grown in hydroponics, root APases increased in Macaca at 1.85 mM Al, whereas decreased at all Al levels in S. microdontum. In greenhouse plantlets, root APases decreased at 7.40 mM Al in S. microdontum and SMIC148-A, and at 3.70, 5.55 and 7.40 mM Al in Dakota Rose. Shoot APases decreased in Macaca and SMIC148-A. Conversely, in Dakota Rose, APases increased at 1.85 and 3.70 mM Al. These results show that the effect of Al toxicity on in vitro APase activity depends not only on Al availability but also on the plant organ, genetic background, and the growth conditions. Therefore, it suggests that acid phosphatases activity assessed in vitro might not be a good parameter to validate the screening for adaptation of potato clones to Al toxicity.

Potato root and leaf phosphatase activity in response to P deprivation

“…The differences between Kuba and Cardoso demonstrate the variability in this trait as was also shown by Tabaldi et al. (2011), who observed differences between cultivars depending on both tissue and growth condition. Since plant phosphatases can have also functions apart from P scavenging and recycling, the overall higher intracellular activity in Kuba could be a signal for a better adaption to potential stress since phosphatases are, for example, involved in P signaling (Del Vecchio et al., 2014), regulation of cell‐wall biosynthesis (Kaida et al., 2009), alkaline peroxidase activity (Del Pozo et al., 1999; Veljanovski et al., 2006), response to wounding (Veljanovski et al., 2010), fungal infection (Zamani et al., 2014), and tolerance to abiotic stress (Zhang et al., 2008).…”

Can species Cedrela fissilis Vell. be used in sites contaminated with toxic aluminum and cadmium metals?

“…4C) and increases water use efficiency, especially at the highest Al concentration (100 mg l -1 ). A possible explanation is that higher Al concentrations in the nutrient solution enhances cuticle and epicuticular wax development and leads to higher WUE (Tabaldi et al 2011). In addition, seedlings exposed to Al showed reduced stomatal conductance, which may also result in higher WUE.…”

The Effect of Mineral Supplementation on the Physiological Quality, Enzymes Activity and the Soybean Seedlings Development

“…Thus, less energy for the maintenance of plant metabolism and initial growth was made available, as also observed in the first count of the germination test and initial seedling growth (Tables 2 and 4). Besides, probably, the lower availability of metal ions in the seeds of the low nutritional lot promoted a reduction in APase activity, since it is a metalloenzyme dependent on Ca 2+ or Mg 2+ (Tabaldi et al, 2011). In the mineral supplementation lots, this reduction in activity may be related to the gradual release of phosphorus (P) from the supplementation, which makes P available in a form readily assimilable by the plant (inorganic form).…”