Effect of Aluminum Chloride on the Organogenesis of Two Types of <i>Cymbidium In Vitro</i>

Abstract

We examined the effects of aluminum chloride (AlCl 3 ) on the organogenesis of Cymbidium Sweet Waffle 'Tarte' from inoculated protocorm-like bodies (PLBs) and of Cymbidium kanran 'Murotonishiki' from rhizomes to determine the appropriate concentration of AlCl 3 for organogenesis. The explants were cultured in modified MS media supplemented with various concentrations of AlCl 3 and maintained at 25Ϯ1℃ and a 24 h light period for 6 weeks for C. Sweet Waffle 'Tarte' and 10 weeks for C. kanran 'Murotonishiki.' In C. Sweet Waffle 'Tarte' 1.0 mg L Ϫ1 AlCl 3 significantly increased PLB formation, shoot formation from PLBs and root formation from shoot. In C. kanran 'Murotonishiki,' 1.0 mg L Ϫ1 AlCl 3 induced shoot from rhizome and developed roots from shoot. On the other hand, high concentration of AlCl 3 (10 mg L Ϫ1 ) resulted the formation of protocorm-like shoots in both Cymbidiums. We concluded that the concentration of 1.0 mg L Ϫ1 AlCl 3 is optimum for the root and shoot formation of the Cymbidium orchids.

Effect of Citric Acid on the Organogenesis of <i>Cymbidium floribundum</i>

“…Thus, the effect of CA on organogenesis in protocorm-like bodies (PLBs) of Cymbidium species remains unknown. However, the organogenetic pathways are different in PLB-forming type and rhizome-forming type Cymbidium (Ona et al, 2022), so this study was undertaken to determine the effects of CA on the regeneration of PLBs and the formation of shoots from PLBs and roots from shoots in PLB culture of C. floribundum in modified MS media (Shimasaki and Uemoto, 1990).…”