Effect of Alpinia oxyphylla extract in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model

Lee, Yun Mi; Son, Eunjung; Kim, Seung-Hyung; Kim, Dong-Seon

doi:10.1016/j.phymed.2019.153095

Cited by 26 publications

(20 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In previous study, we showed that AOE inhibited the degradation of proteoglycan in rabbit articular cartilage explants treated with IL-1α, and this result is in line with another our work showing AOE attenuated proteoglycan depletion in MIA-induced OA rats (Lee et al, 2019;Yu et al, 2020). In this study, we investigated collagen degradation in rabbit articular cartilage explants exposed to IL-1α and plasminogen to further examine potential chondroprotective effect of AOE.…”

Section: Discussionsupporting

confidence: 88%

“…Therefore, this result suggests that AOE has the ability to protect articular cartilage by reducing the degradation not only proteoglycan but also collagen. The content of nootkatone was analyzed because it is a major compound of AOE (Lee et al, 2019;Yu et al, 2020). Nootkatone also showed inhibitory effect on collagen degradation, but its efficacy was weaker than AOE.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, antiinflammatory effect of A. oxyphylla aqueous extract in RAW264.7 cells has been reported (Jo et al, 2017). In our previous study, A. oxyphylla ethanol extract (AOE) showed inhibitory effect on cartilage degradation in a monosodium iodoacetate (MIA)-induced OA animal model and also showed anti-inflammatory and analgesic activities in animal models, and inhibitory effect on proteoglycan degradation from interleukin-1α (IL-1α)-treated rabbit articular cartilage (Lee et al, 2019;Yu et al, 2020). In this study, we investigated the inhibitory effect of AOE on collagen degradation and inflammation against cartilage damage triggered by IL-1α and plasminogen in rabbit articular cartilage explants for further research on potential chondroprotective activity of AOE.…”

mentioning

confidence: 98%

“…In this study, we investigated the inhibitory effect of AOE on collagen degradation and inflammation against cartilage damage triggered by IL-1α and plasminogen in rabbit articular cartilage explants for further research on potential chondroprotective activity of AOE. Follow is the detail of extraction methods (Lee et al, 2019): 200 g of A. oxyphylla dried fruit was purchased from herbal market (Danyang, China) and extracted twice with 50% ethanol (with a 4-hr reflux). After the extract was filtered with filter paper, it was concentrated and dried.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Chondroprotective effect of Alpinia oxyphylla extract in experimentally induced cartilage degradation in rabbit articular cartilage explants

Jeon¹,

Yu²,

Lee³

et al. 2021

J Food Biochem

View full text Add to dashboard Cite

Alpinia oxyphylla is a widely used medicinal herb for diarrhea, gastralgia, tumors, hypertention, and cerebrovascular disorders. Here, we evaluated the chondroprotective effect of A. oxyphylla dried fruit ethanol extract (AOE) against cartilage degradation in rabbit articular cartilage explants. Treatment of interleukin-1α (IL-1α) and plasminogen increased degraded collagen release in culture supernatants, but pretreatment of AOE (50, 100, 200 µg/ml) inhibited the collagen release in dose-dependent manner. To examine the mechanism of action of AOE on chondroprotection, the level of matrix metalloproteinases-3 (MMP-3), matrix metalloproteinases-13 (MMP-13), tissue inhibitor of metalloprotease-1 (TIMP-1), and inflammatory mediators like prostaglandin E 2 (PGE 2 ) and nitric oxide (NO) was evaluated. AOE inhibited upregulation of MMP-3 and MMP-13 and downregulation of TIMP-1 and also reduced increase of PGE 2 and NO level induced by exposure of IL-1α and plasminogen. These results indicate that AOE show chondroprotective effect through inhibiting collagen degradation via regulating MMPs, TIMP-1, and inflammatory mediators. Practical applicationsOsteoarthritis (OA) is a one of the most common chronic disorders in elderly persons.Because the regenerative power of joint articular cartilage is very low, treatment of OA is difficult to expect complete recovery. Therefore, there is a need to develop a therapeutic agent that can safely and effectively inhibit the cartilage destruction.For the first time, we exhibited the inhibitory effect of AOE on collagen degradation through regulating MMPs and TIMP-1 in articular cartilage explants. These findings support AOE could be used as herbal therapeutic application for protecting articular cartilage to prevent OA.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

See 2 more Smart Citations

Chondroprotective effect of Alpinia oxyphylla extract in experimentally induced cartilage degradation in rabbit articular cartilage explants

Jeon¹,

Yu²,

Lee³

et al. 2021

J Food Biochem

View full text Add to dashboard Cite

show abstract

“…Various stressors including cobalt chloride, hydrogen peroxide and Lipopolysaccharide are used to induce oxidative stress in vitro [27][28][29]. Monosodium iodoacetate (MIA) is usually used to induce stress in chondrocytes both in vitro and in vivo [30][31][32][33][34]. MIA promotes ROS level thereby causing membrane potential alterations, upregulates caspase-3 activity and enhances the release of cytochrome c that ultimately leading chondrocyte to apoptosis [35].…”

Section: Introductionmentioning

confidence: 99%

Daphne mucronataenhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stressin vitro

et al. 2020

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are being used to treat many diseases as they exhibit great regenerative potential. However, MSC's transplantation sometimes does not yield the maximum regenerative outcome as they are unable to survive in inflammatory conditions. Several approaches including preconditioning are used to improve the survival rate of mesenchymal stem cells. One such recently reported approach is preconditioning MSCs with plant extracts. The present study was designed to evaluate the effect of Daphne mucronata extract on stressed human adipose-derived mesenchymal stem cells (hADMSCs). Isolated hADMSCs were preconditioned with different concentrations of Daphne muconata extract and the protective, proliferative, antioxidant and anti-inflammatory effect was assessed through various assays and expression analysis of inflammatory markers regulated through NF-κB pathway. Results suggest that preconditioning hADMSCs with Daphne mucronata increased the cell viability, proliferative and protective potential of hADMSCs with a concomitant reduction in LDH, ROS and elevation in SOD activity. Moreover, both the ELISA and gene expression analysis demonstrated down regulations of inflammatory markers (IL1-β, TNF-α, p65, p50, MMP13) in Daphne mucronata preconditioned hADMSCs as compared to stress. This is the first study to report the use of MIA induced oxidative stress against hADMSC's and effect of Daphne mucronata on stressed hADMSCs. Results of these studies provided evidence that Daphne mucronata protects the hADMSCs during stress conditions by down regulating the inflammatory markers and hence increase the viability and proliferative potential of hADMSCs that is crucial for transplantation purposes.

show abstract

Chondroprotective effects of Protaetia brevitarsis seulensis larvae as an edible insect on osteoarthritis in mice

Chun,

Nam,

Lee

et al. 2023

Food Science & Nutrition

View full text Add to dashboard Cite

Osteoarthritis (OA) is a common chronic joint inflammatory disease characterized by progressive destruction of the articular cartilage, bone remodeling, and excessive chronic pain. Most therapeutic approaches do not rescue the progression of OA effectively or provide relief of symptoms. Protaetia brevitarsis seulensis larva (PBSL), which is attracting attention, is an edible insect with very high nutritional value and herbal medicine for the treatment of blood stasis, hepatic disease, and various inflammatory diseases. However, the effect of PBSL on OA has not yet been investigated. This study aimed to demonstrate the effects of PBSL water extract on the progression of OA using monosodium iodoacetate (MIA)‐induced mice and SW1353 chondrocytes or murine macrophages. We injected MIA into the intraarticular area of mice following pretreatment with either saline or PBSL (200 mg/kg) for 2 weeks, and then locomotor activity, microcomputed tomography and histopathological analysis, quantitative reverse transcriptase–polymerase chain reaction analysis, and western blot analysis were performed. To determine the molecular effects of PBSL, we used interleukin‐1β (IL‐1β)‐induced SW1353 chondrosarcoma or lipopolysaccharide (LPS)‐stimulated macrophages. Pretreatment with PBSL diminished the symptoms of OA. Physical activity, articular cartilage damage, and the generation of microfractures were rescued by pretreatment with PBSL in the mouse model. Pretreatment with PBSL suppressed the progress of OA through the regulation of articular cartilage degradation genes and inflammation in both in vivo and in vitro models. Our results demonstrated that PBSL has value as edible insect that can be used in the development of functional foods for OA.

show abstract

Effect of Alpinia oxyphylla extract in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model

Cited by 26 publications

References 25 publications

Chondroprotective effect of Alpinia oxyphylla extract in experimentally induced cartilage degradation in rabbit articular cartilage explants

Chondroprotective effect of Alpinia oxyphylla extract in experimentally induced cartilage degradation in rabbit articular cartilage explants

Daphne mucronataenhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stressin vitro

Chondroprotective effects of Protaetia brevitarsis seulensis larvae as an edible insect on osteoarthritis in mice

Contact Info

Product

Resources

About