Effect of allyl isothiocyanate on developmental toxicity in exposed Xenopus laevis embryos

Williams, John Russell; Rayburn, James R.; Cline, George R.; Sauterer, Roger; Friedman, Mendel

doi:10.1016/j.toxrep.2014.12.005

Cited by 14 publications

(8 citation statements)

References 46 publications

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“…Indeed, much longer exposures than used in the present study (hours-to-days) to AITC can deplete glutathione or other antioxidants (Overby et al, 2015). A reduction in antioxidants and other processes elicited by AITC exposure can have serious adverse effects on development, including malformations of the body in embryos (Williams et al, 2015). Another possible explanation for the behavioral and physiological changes we observed following treatment with AITC is that they were due to minor injury caused by the chemical irritant.…”

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

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Induction of Short-Term Sensitization by an Aversive Chemical Stimulus in Zebrafish Larvae

Roberts

Alzagatiti

et al. 2020

eNeuro

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Larval zebrafish possess a number of molecular and genetic advantages for rigorous biological analyses of learning and memory. These advantages have motivated the search for novel forms of memory in these animals that can be exploited for understanding the cellular and molecular bases of vertebrate memory formation and consolidation. Here, we report a new form of behavioral sensitization in zebrafish larvae that is elicited by an aversive chemical stimulus [allyl isothiocyanate (AITC)] and that persists for ≥30 min. This form of sensitization is expressed as enhanced locomotion and thigmotaxis, as well as elevated heart rate. To characterize the neural basis of this nonassociative memory, we used transgenic zebrafish expressing the fluorescent calcium indicator GCaMP6 ( Chen et al., 2013 ); because of the transparency of larval zebrafish, we could optically monitor neural activity in the brain of intact transgenic zebrafish before and after the induction of sensitization. We found a distinct brain area, previously linked to locomotion, that exhibited persistently enhanced neural activity following washout of AITC; this enhanced neural activity correlated with the behavioral sensitization. These results establish a novel form of memory in larval zebrafish and begin to unravel the neural basis of this memory.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Induction of Short-Term Sensitization by an Aversive Chemical Stimulus in Zebrafish Larvae

Roberts

Alzagatiti

et al. 2020

eNeuro

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“…For concentration‐response studies of toxic effects on embryos, all test concentrations were repeated at least three times. The EC 50 and LC 50 were calculated for each experiment and the teratogenic index was determined as the ratio of LC 50 to EC 50 (Williams, Rayburn, Cline, Sauterer, & Friedman, ). The values of LC 50 and EC 50 were obtained by calculating the average from three different experiments.…”

Section: Methodsmentioning

confidence: 99%

Fluorinated diiodine alkanes exert developmental toxicity on embryo‐larval stages of zebrafish strain AB via regulating the expression of the specific endocrine‐related genes

Lü

Chang

et al. 2019

J of Applied Toxicology

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Fluorinated diiodine alkanes (FDIAs) are environmental pollutants, including octafluoro‐1,4‐diiodobutane (PFBDI), hexadecafluoro‐1,8‐diiodooctane (PFODI) and dodecafluoro‐1,6‐diiodohexane (PFHxDI). They showed an estrogenic effect in in vitro studies. However, little information is currently available regarding the toxicity of FDIAs in in vivo studies. Zebrafish (Danio rerio) is a vertebrate animal model that is increasingly used for toxicity and efficacy screening as well as for assessing the toxicity and safety of novel compounds, pollutants and pharmaceuticals. In the present study, we investigated the developmental toxicity of FDIAs (PFBDI, PFHxDI and PFODI) and the specific endocrine‐related gene expression in zebrafish embryos. The results revealed that all three FDIAs showed developmental toxicity on zebrafish embryos. The half‐maximal effective concentration values for PFBDI, PFHxDI and PFODI were 0.89 ± 0.07, 0.53 ± 0.04 and 0.04 ± 0.007 mm, respectively. PFHxDI exhibited the highest developmental toxicity compared with the other FDIAs. In addition, all three FDIAs significantly upregulated the expression of estrogen receptor (esr)1 and cytochrome P450 (CYP) 19b (CYP19b), but did not significantly affect the expression of esr2b, CYP17 and CYP19a in zebrafish. The upregulation effect of PFHxDI was greater than the effect of PFBDI and PFODI. This study furthers our knowledge on the effects of FDIAs on the developmental toxicity and the specific endocrine‐related gene expression in the embryo‐larval stages of zebrafish. Our results provided a preliminary insight into the toxicity of FDIAs in zebrafish, which will be of great relevance regarding future studies on FDIAs in the environment.

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“…The development of the botanical control method has been proposed as one of the most important strategies for pest management on fruits, vegetables, and ornamental plants ( Zhang et al, 2015 ; Cheng et al, 2018 ; Lu et al, 2020 ). AITC, commonly known as horseradish, is a volatile and aliphatic sulfur-containing compound naturally occurring in plants from the family of Cruciferae, such as: horseradish ( Armoracia rusticana ), mustard ( Brassica nigra ), cabbage ( Brassica oleracea ), and wasabi ( Wasabia japonica ) ( Mayton et al, 1996 ; Wu et al, 2009 ; Williams et al, 2015 ; Li Y et al, 2018 ). AITC could be rapidly adsorbed and degraded in soil with a low risk of persistence ( Ren et al, 2018 ); thus, it has been considered a potential botanical pesticide.…”

Section: Introductionmentioning

confidence: 99%

Responses of Fungi Maggot (Bradysia impatiens Johannsen) to Allyl Isothiocyanate and High CO2

et al. 2022

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Botanical pesticide is highly recommended for integrated pest management (IPM), due to its merits such as environmental friendliness, safe to non-target organisms, operators, animals, and food consumers. The experiment was conducted to determine the lethal and sub-lethal effects of allyl isothiocyanate (AITC) on eggs, third instar larvae, pupae, and females and males of Bradysia impatiens Johannsen (B. impatiens). Different concentrations of AITC under ambient CO2 by the conical flask sealed fumigation method were used for the experiment. The results showed that there was a significant linear relationship between different concentrations of AITC and the toxicity regression equation of B. impatiens. The sub-lethal concentrations of AITC had significant effects on the larval stage, pupal stage, pupation rate, pupal weight, adult emergence rate, and oviposition. The pupation rate, pupal weight, and adult emergency rate were significantly (p < 0.05) affected by AITC fumigation. The pupation rate was the lowest after fumigation treatment of AITC at LC50 (36.67%), followed by LC25 (41.94%), compared with the CK (81.39%). Female longevity was significantly (p < 0.05) shortened by fumigation at LC25 (1.75 d) and LC50 (1.64 d), compared with that of CK (2.94 d). Male longevity was shorter at LC25 (1.56 d) than at LC50 (1.25 d) and had no significant difference between these two treatments. The fumigation efficiency of AITC was significantly increased under high CO2 condition. Furthermore, detoxification enzyme activities and antioxidant enzyme activities were accumulated under high CO2 condition. The fumigation method in the application of AITC can be useful in areas where B. impatiens is a major concern.

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Effect of allyl isothiocyanate on developmental toxicity in exposed Xenopus laevis embryos

Abstract: Graphical abstract

Cited by 14 publications

References 46 publications

Induction of Short-Term Sensitization by an Aversive Chemical Stimulus in Zebrafish Larvae

Induction of Short-Term Sensitization by an Aversive Chemical Stimulus in Zebrafish Larvae

Fluorinated diiodine alkanes exert developmental toxicity on embryo‐larval stages of zebrafish strain AB via regulating the expression of the specific endocrine‐related genes

Responses of Fungi Maggot (Bradysia impatiens Johannsen) to Allyl Isothiocyanate and High CO2

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