Effect of allyl isothiocyanate on ultra-structure and the activities of four enzymes in adult Sitophilus zeamais

Wu, Hua; Liu, Xueru; Yu, Dongdong; Zhang, Xing; Feng, Juntao

doi:10.1016/j.pestbp.2014.01.001

Cited by 24 publications

(26 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AITC has been used as a fumigant for many stored product pest insects [25][26][27]. Worfel et al (1997) demonstrated that AITC reduced the reproduction in Lasioderma serricorne in a warehouse and also reduced the activity and reproduction of Tribolium confusum [4].…”

Section: Discussionmentioning

confidence: 99%

Insecticidal and Enzyme Inhibitory Activities of Isothiocyanates against Red Imported Fire Ants, Solenopsis invicta

Grodowitz

Chen

2020

Biomolecules

View full text Add to dashboard Cite

Contact and fumigation toxicity of four isothiocyanates (ITCs), including allyl isothiocyanate (AITC), 3-butenyl isothiocyanate (3BITC), 3-(methylthio) propyl isothiocyanate (3MPITC) and 2-phenylethyl isothiocyanate (2PEITC), were evaluated against the red imported fire ant worker, Solenopsis invicta Buren. 2PEITC and 3MPITC exhibited strong contact toxicity. The median lethal dose (LD50)value of AITC, 2PEITC and 3MPITC were 7.99, 2.36 and 2.09 µg/ant respectively. In addition, AITC and 3MPITC also showed strong fumigation toxicity but not 2PEITC. The median lethal concentration (LC50) values of AITC and 3MPITC were 32.49 and 57.6 µg/L, respectively. In contrast, 3BITC did not exhibit any contact and fumigation toxicity even at 100 μg/μL. Esterase (EST), glutathione S-transferase (GST) and acetylcholinesterase (AChE)-inhibiting activities were assessed for three ITCs in S. invicta workers. All three ITCs inhibited both EST and GST activities but not AChE. The in vitro half maximal inhibitory concentration (IC50)values of AITC, 2PEITC and 3MPITC for GST were 3.32, 0.61 and 0.66 µg/µL, respectively. These results suggested that naturally occurring ITCs might be potentially useful for developing fire ants control products.

show abstract

Section: Discussionmentioning

confidence: 99%

Insecticidal and Enzyme Inhibitory Activities of Isothiocyanates against Red Imported Fire Ants, Solenopsis invicta

Grodowitz

Chen

2020

Biomolecules

View full text Add to dashboard Cite

show abstract

“…Elevated level of mixed function oxidases has been involved in imparting resistance to organochlorines, pyrethroids, and neonicotinoids in many insect species (Malathi et al, 2017). The mixedfunction oxidase activities are involved in the respiratory electron transport chain (Wu et al, 2014). The insecticide agents in plant may act by inhibiting the mitochondrial electron transport (Rattan, 2010).…”

Section: Resultsmentioning

confidence: 99%

Galls of Guiera senegalensis J.F. GMEL (Combretaceae) as potential source of insecticides against the main cowpea pest Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae) detoxification enzymes

Sombié¹,

Coulibaly²,

Zongo³

et al. 2018

Afr. Crop Sci. J.

View full text Add to dashboard Cite

The cowpea weevil, Callosobruchus maculatus (F.), is a major pest of cowpea (Vigna unguiculata (L.) Walp.) in storage. A laboratory study was conducted to investigate the inhibition potential of Guiera senegalensis galls on C. maculatus detoxification enzymes. Methanol extract from galls of G. senegalensis exhibited strong inhibitory effects of the activity of C. maculatus acetylcholinesterase (AChE) and mixed-functional oxidase enzymes. The chemical composition of the extracted oils was examined by gas chromatograph (GC)-flame ionisation detectors (FID) and mass spectrometry. Twelve components were identified, namely 2-Hydroxy-4-methoxybenzophenone, n-Pentadecane, Octadecane, 1,2-Benzenedicarboxylic acid, dibutyl ester, Palmitic Acid, Hexadecane, Cis-vaccenic acid, Oleyl alcohol, stenol, n-Eicosane, n-Heneicosane and vincubine. This plant could be used for improving the efficacy to various insecticide classes against C. maculatus and as an alternative to synthetic insecticides.

show abstract

“…Further, we reported a yeast-like vacuolar transient receptor potential channel regulator (FsYvc1), and found that loss of FsYvc1 resulted in hypersensitivity of F. solani to AITC, suggesting FsYvc1 should be act as a biosensor in sensing AITC. The mitochondria are likely the main target of AITC, as AITC had a significant effect on the mitochondrial respiratory chain of S. zeamais in vivo and in vitro (Hua et al, 2014;Zhang et al, 2016). Similarly, ITCs caused to a decreased oxygen consumption rate, intracellular accumulation of ROS, and mitochondrial membrane depolarization in A. brassicicola FIGURE 4 | Comparison of biological characters between WT and FsYvc1-17: (A) Colony growth (n = 6 for each plot, **p < 0.01, ±SD).…”

Section: Discussionmentioning

confidence: 99%

Allyl Isothiocyanate (AITC) Triggered Toxicity and FsYvc1 (a STRPC Family Member) Responded Sense in Fusarium solani

Liu

Zhang

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Allyl isothiocyanate (AITC) is a natural product used as a food additive. Due to its strong volatility and broad biological activity, AITC is considered as a bio-fumigant to control soil-borne fungal diseases in agriculture, creating an urgent need for evaluation of the antifungal activity of AITC. Here we study the effect of AITC on Fusarium solani growth and explore the molecular mechanisms. The results indicated that AITC causes rapid inhibition of F. solani after 5 min, hyphal deformity, and electrolyte leakage. A yeast-like vacuolar transient receptor potential channel regulator (FsYvc1, a STRPC family member) was identified in F. solani that seems to play a role in this fungi AITC sensitivity. Genetic evidence suggests the gene FsYvc1 is involved in F. solani growth, development, and pathogenicity. Loss of FsYvc1 resulted in hypersensitivity of F. solani to AITC and induced reactive oxygen species (ROS) accumulation ∼ 1.3 to 1.45-folds that of the wild type (WT), and no difference responses to CaCl 2 , NaCl, KCl, SDS, and Congo red when compared with WT. In addition, FsYvc1-17 showed significantly reduced (∼ 1-fold) glutathione-S-transferase (GST) expression compared with the WT without AITC induction. Upon exposure to 4.8 µg/mL AITC for 3 h, the relative expression levels were ∼ 12-30 fold higher in both the WT and FsYvc1-17. Nevertheless, no difference in GST expression level was observed between the WT and FsYvc1-17. The current study provides novel insights into the toxicity mechanisms of AITC. Considering our results that show the key role of FsYvc1, we propose that it could act as a new molecular target for future fungicide development.

show abstract

Effect of allyl isothiocyanate on ultra-structure and the activities of four enzymes in adult Sitophilus zeamais

Cited by 24 publications

References 21 publications

Insecticidal and Enzyme Inhibitory Activities of Isothiocyanates against Red Imported Fire Ants, Solenopsis invicta

Insecticidal and Enzyme Inhibitory Activities of Isothiocyanates against Red Imported Fire Ants, Solenopsis invicta

Galls of Guiera senegalensis J.F. GMEL (Combretaceae) as potential source of insecticides against the main cowpea pest Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae) detoxification enzymes

Allyl Isothiocyanate (AITC) Triggered Toxicity and FsYvc1 (a STRPC Family Member) Responded Sense in Fusarium solani

Contact Info

Product

Resources

About