Effect of alkylhydroxybenzenes, microbial anabiosis inducers, on the structural organization of Pseudomonas aurantiaca DNA and on the induction of phenotypic dissociation

Mulyukin, A. L.; Vakhrushev, M. A.; Strazhevskaya, N. B.; Shmyrina, A. S.; Жданов, Р. И.; Suzina, N. E.; Дуда, В. И.; Kozlova, A. N.; Эль-Регистан, Г. И.

doi:10.1007/s11021-005-0041-8

Cited by 11 publications

(10 citation statements)

References 7 publications

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“…This value is in accordance with previously reported viscoelasticity values for 12 genomic DNAs, prepared by the technique (Struchkov et al , 2002). DNA preparations isolated from P. aurantiaca culture of different physiological states (growing or the stationary‐phase one) display different viscoelasticity values and reflect a level of supercoiling (Mulyukin et al , 2005). A viscoelasticity value of DNA preparation isolated from growing P. aurantiaca cells (exponential phase) was lower by 30%.…”

Section: Resultsmentioning

confidence: 99%

“…aurantiaca B‐1558 (Nakhimovskaya 48) (All‐Russian Collection of Microorganisms, Pushchino, Moscow region). Stationary‐phase cultures (48 h cultivation) of P. aurantiaca cells were grown on a synthetic medium (Mulyukin et al , 2005) of the following composition (g L −1 ): glucose, 2; KH 2 PO 4 , 0.1; (NH 4 ) 2 SO 4 , 0.5; K 2 HPO 4 ·3H 2 O, 1.0; CaCl 2 , 0.2; MgSO 4 ·7H 2 O, 0.1; and yeast extract (Difco), 0.05. The medium also contained microelements (mg L −1 ): FeSO 4 ·7H 2 O, 20; MnCl 2 ·4H 2 O, 20; ZnSO 4 ·7H 2 O, 0.4; B(OH) 3 , 0.5; CuSO 4 ·5H 2 O, 0.05; and Na 2 MoO 4 ·2H 2 O, 0.2; the pH value was 7.25 upon sterilization.…”

Section: Methodsmentioning

confidence: 99%

“…A stationary‐phase culture grown on nutrient broth was used as an inoculum. The inoculum amount provided an initial OD equaling 0.1 (λ=650 nm, L =10 mm, Specord, Carl Zeiss, Germany) (Mulyukin et al , 2005) of the culture suspension.…”

Section: Methodsmentioning

confidence: 99%

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Nature of DNA-bound fatty acids inPseudomonas aurantiaca

Жданов

Shmyrina

Mulyukin

et al. 2006

FEMS Microbiology Letters

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The existence of Pseudomonas aurantiaca DNA-bound fatty acids and lipids is presented in this work. The isolation of DNA was carried out by two different procedures, namely, phenol and detergent-based phenol isolation in order to prove the presence of DNA-bound lipids. The lipid content of DNA is expressed in terms of fatty acid profile. A high level of 16:0, 18:0 and 18:1 is characteristic for tightly bound DNA lipids. On the other hand, the fatty acids such as 14:1, iso14:0 and iso16:0 are found in trace amounts only in DNA lipid fraction, but these fatty acids are not found in the whole-cell lipids. Absolutely no 3-hydroxy fatty acids were found in DNA lipids. However, both C16 and C18 species represent the main fatty acids of whole-cell and DNA-bound lipids. The presence of DNA-bound lipids even under tough treatment of DNA allows to conclude that these lipids represent a special pool among cellular lipids.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Nature of DNA-bound fatty acids inPseudomonas aurantiaca

Жданов

Shmyrina

Mulyukin

et al. 2006

FEMS Microbiology Letters

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“…Cystlike cells of M. luteus, A. globiformis, and P. aurantiaca were obtained after long-term incubation (3-9 months), at room temperature, of microbial cultures grown in media containing amounts of carbon, phosphorus, or nitrogen 10 times lower than in standard media (Mulyukin et al, 1996;Demkina et al, 2000;Suzina et al, 2004;Mulyukin et al, 2005). Cystlike cells of N. pallidum were produced in high-cell-density suspensions starved in highosmolarity buffered media and stored at ambient temperature.…”

Section: Selected Microorganismsmentioning

confidence: 99%

The structural bases of long-term anabiosis in non-spore-forming bacteria

Suzina

Mulyukin

Дмитриев

et al. 2006

Advances in Space Research

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“…Analysis of the fatty acid methyl ester (FAME) profiles of bacteria is widely used for rapid determination of the fatty acid composition of lipids [3]. Some researchers studied the influence of environmental factors on the growth of bacterial strains by changes in their FAME profiles [4][5][6]. In some cases, specific markers of the influence of environmental factors on bacterial strains were found, which are commonly used to identify strains under different conditions [2,7].…”

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confidence: 99%